Native-feather degradation by Fervidobacterium islandicum AW-1, a newly isolated keratinase-producing thermophilic anaerobe

Gae Won Nam, Dong Woo Lee, Han Seoung Lee, Nam Ju Lee, Byoung Chan Kim, Eun Ah Choe, Jae-Kwan Hwang, Maggy T. Suhartono, Yu Ryang Pyun

Research output: Contribution to journalArticle

153 Citations (Scopus)

Abstract

A native-feather-degrading thermophilic anaerobe was isolated from a geothermal hot stream in Indonesia. Isolate AW-1, identified as a member of the species Fervidobacterium islandicum, was shown to degrade native feathers (0.8%, w/v) completely at 70°C and pH 7 with a maximum specific growth rate (0.14 h-1) in Thermotoga-Fervidobacterium (TF) medium. After 24 h of culture, feather degradation led to an increase in free amino acids such as histidine, cysteine and lysine. Moreover, nutritionally essential amino acids such as tryptophan and methionine, which are rare in feather keratin, were also produced as microbial metabolites. A homomultimeric membrane-bound keratinolytic protease (>200 kDa; 97 kDa subunits) was purified from a cell extract of F. islandicum AW-1. The enzyme exhibited activity toward casein and soluble keratin optimally at 100°C and pH 9, and had a half-life of 90 min at 100°C. The enzyme showed higher specific activity for the keratinous substrates than other proteases and catalyzed the cleavage of peptide bonds more rapidly following the reduction of disulfide bridges in feather keratin by 10 mM dithiothreitol. Therefore, the enzyme from F. islandicum AW-1 is a novel, thermostable keratinolytic serine protease.

Original languageEnglish
Pages (from-to)538-547
Number of pages10
JournalArchives of Microbiology
Volume178
Issue number6
DOIs
Publication statusPublished - 2002 Nov 26

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Feathers
Keratins
Peptide Hydrolases
Keratin-10
Degradation
Essential Amino Acids
Dithiothreitol
Enzyme activity
Serine Proteases
Enzymes
Metabolites
Caseins
Histidine
Tryptophan
Disulfides
Methionine
Lysine
Cysteine
Membranes
Amino Acids

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Biochemistry
  • Molecular Biology
  • Genetics

Cite this

Nam, Gae Won ; Lee, Dong Woo ; Lee, Han Seoung ; Lee, Nam Ju ; Kim, Byoung Chan ; Choe, Eun Ah ; Hwang, Jae-Kwan ; Suhartono, Maggy T. ; Pyun, Yu Ryang. / Native-feather degradation by Fervidobacterium islandicum AW-1, a newly isolated keratinase-producing thermophilic anaerobe. In: Archives of Microbiology. 2002 ; Vol. 178, No. 6. pp. 538-547.
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Native-feather degradation by Fervidobacterium islandicum AW-1, a newly isolated keratinase-producing thermophilic anaerobe. / Nam, Gae Won; Lee, Dong Woo; Lee, Han Seoung; Lee, Nam Ju; Kim, Byoung Chan; Choe, Eun Ah; Hwang, Jae-Kwan; Suhartono, Maggy T.; Pyun, Yu Ryang.

In: Archives of Microbiology, Vol. 178, No. 6, 26.11.2002, p. 538-547.

Research output: Contribution to journalArticle

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