Necrotic cell death induces melanotic mass formation in Drosophila

Jin Young Park, Ji Hyun Lee, Youngbin Lee, Donghoon Lee, Moon Jong Kim, Kwang Min Choe

Research output: Contribution to journalArticlepeer-review

Abstract

The immune system protects its host from not only invading parasites and parasitoids, but also altered self tissue, including dying cells. Necrotic cells are strongly immunogenic, but in Drosophila this has not been directly addressed, due partially to the fact that knowledge about necrosis in Drosophila currently lags behind that for other models. Upon the loss of cell matrix attachment, endocycling polyploid tissues of the Drosophila larva undergo autophagy instead of apoptosis; we employed this system as a model to examine cell death modalities and immunity. Here, we report that larval fat body cells depleted of integrin undergo not only autophagy, but also necrotic cell death, and that a blockade of reaper, grim, hid, or the downstream caspases enhances necrosis. These cells elicit melanotic mass formation, an autoimmune-like response. We also show that necrosis is the main cause of melanotic mass formation in these anchorage-depleted polyploid cells.

Original languageEnglish
Pages (from-to)1106-1111
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume526
Issue number4
DOIs
Publication statusPublished - 2020 Jun 11

Bibliographical note

Funding Information:
We thank I. Ando, L. Liu, J. Park, and Bloomington Drosophila Stock Center for fly stocks and reagents. We also thank our laboratory colleagues for helpful discussions. This work was supported by funding from the National Research Foundation of Korea ( NRF-2015R1A2A2A01006660 ) to KMC, and by the Yonsei University Research Fund of 2014 to MJK.

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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