Neoplastic transformation and tumorigenesis associated with overexpression of phospholipase D isozymes in cultured murine fibroblasts

Do Sik Min, Taeg Kyu Kwon, Won Sang Park, Jong Soo Chang, Seung Kiel Park, Bong Hyun Ahn, Zae Young Ryoo, Young Han Lee, Yeon Soo Lee, Duck Joo Rhie, Shin Lee Yoon, Sang June Hahn, Myuk Suk Kim, Yang Hyeok Jo

Research output: Contribution to journalArticle

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Abstract

Phospholipase D (PLD) has been suggested to play an important role in a variety of cellular functions. PLD activity has been shown to be significantly elevated in many tumours and transformed cells, suggesting the possibility that PLD might be involved in tumorigenesis. In this study, we have established stable cell lines overexpressing PLD1 and PLD2 from fibroblast cells. These cells, but not control cells, showed altered growth properties and anchorage-independent growth in soft agar. Both PLD1 and PLD2 also induced an up-regulation of the activity of matrix metalloprotease-9 as detected by zymograms. Furthermore, both PLD1 and PLD2 transformants, but not vector-transfectants, induced undifferentiated sarcoma when transplanted into nude mice. Both PLD1- and PLD2-mediated cell cycle distributions in stable cell lines revealed an increased fraction of cells in the S phase compared with control cells. Interestingly, the level of cyclin D3 protein, known as an activator of G 1 to S phase transition in the cell cycle, was aberrantly high in cells overexpressing PLD1 and PLD2 compared with control cells. These results suggest that overexpression of PLD isozymes may play an important role in neoplastic transformation.

Original languageEnglish
Pages (from-to)1641-1647
Number of pages7
JournalCarcinogenesis
Volume22
Issue number10
Publication statusPublished - 2001 Oct 22

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Phospholipase D
Isoenzymes
Carcinogenesis
Fibroblasts
S Phase
Cell Cycle
Cyclin D3
Cell Line
Phase Transition
Metalloproteases
Growth
Nude Mice
Sarcoma
Agar
Up-Regulation

All Science Journal Classification (ASJC) codes

  • Cancer Research

Cite this

Min, D. S., Kwon, T. K., Park, W. S., Chang, J. S., Park, S. K., Ahn, B. H., ... Jo, Y. H. (2001). Neoplastic transformation and tumorigenesis associated with overexpression of phospholipase D isozymes in cultured murine fibroblasts. Carcinogenesis, 22(10), 1641-1647.
Min, Do Sik ; Kwon, Taeg Kyu ; Park, Won Sang ; Chang, Jong Soo ; Park, Seung Kiel ; Ahn, Bong Hyun ; Ryoo, Zae Young ; Lee, Young Han ; Lee, Yeon Soo ; Rhie, Duck Joo ; Yoon, Shin Lee ; Hahn, Sang June ; Kim, Myuk Suk ; Jo, Yang Hyeok. / Neoplastic transformation and tumorigenesis associated with overexpression of phospholipase D isozymes in cultured murine fibroblasts. In: Carcinogenesis. 2001 ; Vol. 22, No. 10. pp. 1641-1647.
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abstract = "Phospholipase D (PLD) has been suggested to play an important role in a variety of cellular functions. PLD activity has been shown to be significantly elevated in many tumours and transformed cells, suggesting the possibility that PLD might be involved in tumorigenesis. In this study, we have established stable cell lines overexpressing PLD1 and PLD2 from fibroblast cells. These cells, but not control cells, showed altered growth properties and anchorage-independent growth in soft agar. Both PLD1 and PLD2 also induced an up-regulation of the activity of matrix metalloprotease-9 as detected by zymograms. Furthermore, both PLD1 and PLD2 transformants, but not vector-transfectants, induced undifferentiated sarcoma when transplanted into nude mice. Both PLD1- and PLD2-mediated cell cycle distributions in stable cell lines revealed an increased fraction of cells in the S phase compared with control cells. Interestingly, the level of cyclin D3 protein, known as an activator of G 1 to S phase transition in the cell cycle, was aberrantly high in cells overexpressing PLD1 and PLD2 compared with control cells. These results suggest that overexpression of PLD isozymes may play an important role in neoplastic transformation.",
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Min, DS, Kwon, TK, Park, WS, Chang, JS, Park, SK, Ahn, BH, Ryoo, ZY, Lee, YH, Lee, YS, Rhie, DJ, Yoon, SL, Hahn, SJ, Kim, MS & Jo, YH 2001, 'Neoplastic transformation and tumorigenesis associated with overexpression of phospholipase D isozymes in cultured murine fibroblasts', Carcinogenesis, vol. 22, no. 10, pp. 1641-1647.

Neoplastic transformation and tumorigenesis associated with overexpression of phospholipase D isozymes in cultured murine fibroblasts. / Min, Do Sik; Kwon, Taeg Kyu; Park, Won Sang; Chang, Jong Soo; Park, Seung Kiel; Ahn, Bong Hyun; Ryoo, Zae Young; Lee, Young Han; Lee, Yeon Soo; Rhie, Duck Joo; Yoon, Shin Lee; Hahn, Sang June; Kim, Myuk Suk; Jo, Yang Hyeok.

In: Carcinogenesis, Vol. 22, No. 10, 22.10.2001, p. 1641-1647.

Research output: Contribution to journalArticle

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T1 - Neoplastic transformation and tumorigenesis associated with overexpression of phospholipase D isozymes in cultured murine fibroblasts

AU - Min, Do Sik

AU - Kwon, Taeg Kyu

AU - Park, Won Sang

AU - Chang, Jong Soo

AU - Park, Seung Kiel

AU - Ahn, Bong Hyun

AU - Ryoo, Zae Young

AU - Lee, Young Han

AU - Lee, Yeon Soo

AU - Rhie, Duck Joo

AU - Yoon, Shin Lee

AU - Hahn, Sang June

AU - Kim, Myuk Suk

AU - Jo, Yang Hyeok

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N2 - Phospholipase D (PLD) has been suggested to play an important role in a variety of cellular functions. PLD activity has been shown to be significantly elevated in many tumours and transformed cells, suggesting the possibility that PLD might be involved in tumorigenesis. In this study, we have established stable cell lines overexpressing PLD1 and PLD2 from fibroblast cells. These cells, but not control cells, showed altered growth properties and anchorage-independent growth in soft agar. Both PLD1 and PLD2 also induced an up-regulation of the activity of matrix metalloprotease-9 as detected by zymograms. Furthermore, both PLD1 and PLD2 transformants, but not vector-transfectants, induced undifferentiated sarcoma when transplanted into nude mice. Both PLD1- and PLD2-mediated cell cycle distributions in stable cell lines revealed an increased fraction of cells in the S phase compared with control cells. Interestingly, the level of cyclin D3 protein, known as an activator of G 1 to S phase transition in the cell cycle, was aberrantly high in cells overexpressing PLD1 and PLD2 compared with control cells. These results suggest that overexpression of PLD isozymes may play an important role in neoplastic transformation.

AB - Phospholipase D (PLD) has been suggested to play an important role in a variety of cellular functions. PLD activity has been shown to be significantly elevated in many tumours and transformed cells, suggesting the possibility that PLD might be involved in tumorigenesis. In this study, we have established stable cell lines overexpressing PLD1 and PLD2 from fibroblast cells. These cells, but not control cells, showed altered growth properties and anchorage-independent growth in soft agar. Both PLD1 and PLD2 also induced an up-regulation of the activity of matrix metalloprotease-9 as detected by zymograms. Furthermore, both PLD1 and PLD2 transformants, but not vector-transfectants, induced undifferentiated sarcoma when transplanted into nude mice. Both PLD1- and PLD2-mediated cell cycle distributions in stable cell lines revealed an increased fraction of cells in the S phase compared with control cells. Interestingly, the level of cyclin D3 protein, known as an activator of G 1 to S phase transition in the cell cycle, was aberrantly high in cells overexpressing PLD1 and PLD2 compared with control cells. These results suggest that overexpression of PLD isozymes may play an important role in neoplastic transformation.

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