Nerve growth factor and expression of its receptors in patients with diabetic neuropathy

HyeonChang Kim, Y. J. Cho, C. W. Ahn, K. S. Park, J. C. Kim, J. S. Nam, Y. S. Im, Jongeun Lee, Sungchul Lee, H. K. Lee

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Aims Low serum nerve growth factor (NGF) levels have been reported in patients with diabetic peripheral neuropathy (DPN), but the role of NGF in the development of neuropathy is unclear. Thus, we investigated the associations of serum NGF level and NGF receptor activity with the presence and severity of DPN. Methods One hundred and thirty-six patients with Type 2 diabetes were included in this cross-sectional study. Serum NGF levels were measured by ELISA. Expressions of NGF receptors (TrkA and p75NTR) were measured by immunohistochemical staining. The presence and severity of DPN were assessed by neuropathy disability score (NDS) and by corneal nerve fibre length (cNFL) and nerve branch density (cNBD) using in vivo confocal microscopy. Results Patients with DPN had higher serum NGF levels (56-451 pg/ml) than patients without DPN (4-54 pg/ml). However, in DPN patients, serum NGF was negatively associated with neuropathy severity (mild 222 ± 64 pg/ml; moderate 114 ± 17 pg/ml; severe 89 ± 20 pg/ml). This negative association was consistent in all severity indices (NDS, P < 0.001; cNFL, P < 0.001; cNBD P = 0.010) even after adjustment for age, sex, diabetes duration, insulin use, fasting glucose and glycated haemoglobin. Although NGF receptor activities had significantly (P < 0.05) negative associations with the presence and severity of neuropathy, these associations were not significant when adjusted for other factors. Conclusions Serum NGF level was positively associated with the presence of DPN but negatively associated with neuropathy severity in DPN patients. The change in serum NGF might be a consequence of, rather than a contributor to, the early development of DPN.

Original languageEnglish
Pages (from-to)1228-1234
Number of pages7
JournalDiabetic Medicine
Volume26
Issue number12
DOIs
Publication statusPublished - 2009 Dec 1

Fingerprint

Diabetic Neuropathies
Peripheral Nervous System Diseases
Nerve Growth Factor
Serum
Nerve Growth Factor Receptor
Nerve Fibers
Nerve Growth Factor Receptors
Glycosylated Hemoglobin A
Confocal Microscopy
Type 2 Diabetes Mellitus
Fasting
Cross-Sectional Studies
Enzyme-Linked Immunosorbent Assay
Insulin
Staining and Labeling
Glucose

All Science Journal Classification (ASJC) codes

  • Internal Medicine
  • Endocrinology, Diabetes and Metabolism
  • Endocrinology

Cite this

Kim, H., Cho, Y. J., Ahn, C. W., Park, K. S., Kim, J. C., Nam, J. S., ... Lee, H. K. (2009). Nerve growth factor and expression of its receptors in patients with diabetic neuropathy. Diabetic Medicine, 26(12), 1228-1234. https://doi.org/10.1111/j.1464-5491.2009.02856.x
Kim, HyeonChang ; Cho, Y. J. ; Ahn, C. W. ; Park, K. S. ; Kim, J. C. ; Nam, J. S. ; Im, Y. S. ; Lee, Jongeun ; Lee, Sungchul ; Lee, H. K. / Nerve growth factor and expression of its receptors in patients with diabetic neuropathy. In: Diabetic Medicine. 2009 ; Vol. 26, No. 12. pp. 1228-1234.
@article{053278898f5f40e5a09a7b0dd849bdcb,
title = "Nerve growth factor and expression of its receptors in patients with diabetic neuropathy",
abstract = "Aims Low serum nerve growth factor (NGF) levels have been reported in patients with diabetic peripheral neuropathy (DPN), but the role of NGF in the development of neuropathy is unclear. Thus, we investigated the associations of serum NGF level and NGF receptor activity with the presence and severity of DPN. Methods One hundred and thirty-six patients with Type 2 diabetes were included in this cross-sectional study. Serum NGF levels were measured by ELISA. Expressions of NGF receptors (TrkA and p75NTR) were measured by immunohistochemical staining. The presence and severity of DPN were assessed by neuropathy disability score (NDS) and by corneal nerve fibre length (cNFL) and nerve branch density (cNBD) using in vivo confocal microscopy. Results Patients with DPN had higher serum NGF levels (56-451 pg/ml) than patients without DPN (4-54 pg/ml). However, in DPN patients, serum NGF was negatively associated with neuropathy severity (mild 222 ± 64 pg/ml; moderate 114 ± 17 pg/ml; severe 89 ± 20 pg/ml). This negative association was consistent in all severity indices (NDS, P < 0.001; cNFL, P < 0.001; cNBD P = 0.010) even after adjustment for age, sex, diabetes duration, insulin use, fasting glucose and glycated haemoglobin. Although NGF receptor activities had significantly (P < 0.05) negative associations with the presence and severity of neuropathy, these associations were not significant when adjusted for other factors. Conclusions Serum NGF level was positively associated with the presence of DPN but negatively associated with neuropathy severity in DPN patients. The change in serum NGF might be a consequence of, rather than a contributor to, the early development of DPN.",
author = "HyeonChang Kim and Cho, {Y. J.} and Ahn, {C. W.} and Park, {K. S.} and Kim, {J. C.} and Nam, {J. S.} and Im, {Y. S.} and Jongeun Lee and Sungchul Lee and Lee, {H. K.}",
year = "2009",
month = "12",
day = "1",
doi = "10.1111/j.1464-5491.2009.02856.x",
language = "English",
volume = "26",
pages = "1228--1234",
journal = "Diabetic Medicine",
issn = "0742-3071",
publisher = "Wiley-Blackwell",
number = "12",

}

Nerve growth factor and expression of its receptors in patients with diabetic neuropathy. / Kim, HyeonChang; Cho, Y. J.; Ahn, C. W.; Park, K. S.; Kim, J. C.; Nam, J. S.; Im, Y. S.; Lee, Jongeun; Lee, Sungchul; Lee, H. K.

In: Diabetic Medicine, Vol. 26, No. 12, 01.12.2009, p. 1228-1234.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Nerve growth factor and expression of its receptors in patients with diabetic neuropathy

AU - Kim, HyeonChang

AU - Cho, Y. J.

AU - Ahn, C. W.

AU - Park, K. S.

AU - Kim, J. C.

AU - Nam, J. S.

AU - Im, Y. S.

AU - Lee, Jongeun

AU - Lee, Sungchul

AU - Lee, H. K.

PY - 2009/12/1

Y1 - 2009/12/1

N2 - Aims Low serum nerve growth factor (NGF) levels have been reported in patients with diabetic peripheral neuropathy (DPN), but the role of NGF in the development of neuropathy is unclear. Thus, we investigated the associations of serum NGF level and NGF receptor activity with the presence and severity of DPN. Methods One hundred and thirty-six patients with Type 2 diabetes were included in this cross-sectional study. Serum NGF levels were measured by ELISA. Expressions of NGF receptors (TrkA and p75NTR) were measured by immunohistochemical staining. The presence and severity of DPN were assessed by neuropathy disability score (NDS) and by corneal nerve fibre length (cNFL) and nerve branch density (cNBD) using in vivo confocal microscopy. Results Patients with DPN had higher serum NGF levels (56-451 pg/ml) than patients without DPN (4-54 pg/ml). However, in DPN patients, serum NGF was negatively associated with neuropathy severity (mild 222 ± 64 pg/ml; moderate 114 ± 17 pg/ml; severe 89 ± 20 pg/ml). This negative association was consistent in all severity indices (NDS, P < 0.001; cNFL, P < 0.001; cNBD P = 0.010) even after adjustment for age, sex, diabetes duration, insulin use, fasting glucose and glycated haemoglobin. Although NGF receptor activities had significantly (P < 0.05) negative associations with the presence and severity of neuropathy, these associations were not significant when adjusted for other factors. Conclusions Serum NGF level was positively associated with the presence of DPN but negatively associated with neuropathy severity in DPN patients. The change in serum NGF might be a consequence of, rather than a contributor to, the early development of DPN.

AB - Aims Low serum nerve growth factor (NGF) levels have been reported in patients with diabetic peripheral neuropathy (DPN), but the role of NGF in the development of neuropathy is unclear. Thus, we investigated the associations of serum NGF level and NGF receptor activity with the presence and severity of DPN. Methods One hundred and thirty-six patients with Type 2 diabetes were included in this cross-sectional study. Serum NGF levels were measured by ELISA. Expressions of NGF receptors (TrkA and p75NTR) were measured by immunohistochemical staining. The presence and severity of DPN were assessed by neuropathy disability score (NDS) and by corneal nerve fibre length (cNFL) and nerve branch density (cNBD) using in vivo confocal microscopy. Results Patients with DPN had higher serum NGF levels (56-451 pg/ml) than patients without DPN (4-54 pg/ml). However, in DPN patients, serum NGF was negatively associated with neuropathy severity (mild 222 ± 64 pg/ml; moderate 114 ± 17 pg/ml; severe 89 ± 20 pg/ml). This negative association was consistent in all severity indices (NDS, P < 0.001; cNFL, P < 0.001; cNBD P = 0.010) even after adjustment for age, sex, diabetes duration, insulin use, fasting glucose and glycated haemoglobin. Although NGF receptor activities had significantly (P < 0.05) negative associations with the presence and severity of neuropathy, these associations were not significant when adjusted for other factors. Conclusions Serum NGF level was positively associated with the presence of DPN but negatively associated with neuropathy severity in DPN patients. The change in serum NGF might be a consequence of, rather than a contributor to, the early development of DPN.

UR - http://www.scopus.com/inward/record.url?scp=70549113396&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=70549113396&partnerID=8YFLogxK

U2 - 10.1111/j.1464-5491.2009.02856.x

DO - 10.1111/j.1464-5491.2009.02856.x

M3 - Article

C2 - 20002474

AN - SCOPUS:70549113396

VL - 26

SP - 1228

EP - 1234

JO - Diabetic Medicine

JF - Diabetic Medicine

SN - 0742-3071

IS - 12

ER -