New degradable cationic ester lipids with 'T-shape' configurations were synthesized and tested for gene delivery carrier. Their transfection efficiency and toxicity were compared with commercially available cationic lipids, DOTMA, DOSPA, and DC-Chol. They showed efficient transfection activity and almost no toxicity on mammalian cell lines. Their ester bond degradation was monitored by 1H NMR.
Bibliographical noteFunding Information:
We acknowledge the support of this work by the Korea Research Foundation (2001-015-DP0344), The Korea Science and Engineering Foundation (R02-2002-000-00011-0), and the SRL-Molecular Therapy Research Center, Sunkyunkwan University. We thank Hoyoung Song and Han Kim for their insightful discussions on the synthesis of lipids.
All Science Journal Classification (ASJC) codes
- Molecular Medicine
- Molecular Biology
- Pharmaceutical Science
- Drug Discovery
- Clinical Biochemistry
- Organic Chemistry