TY - JOUR
T1 - New cfiA variant and novel insertion sequence elements in carbapenem-resistant Bacteroides fragilis isolates from Korea
AU - Roh, Kyoung Ho
AU - Kim, Sinyoung
AU - Kim, Chang Ki
AU - Yum, Jong Hwa
AU - Kim, Myung Sook
AU - Yong, Dongeun
AU - Jeong, Seok Hoon
AU - Lee, Kyungwon
AU - Kim, June Myung
AU - Chong, Yunsop
PY - 2010/4
Y1 - 2010/4
N2 - Of 276 nonduplicate Bacteroides fragilis clinical isolates recovered from 1997 to 2004, 3 were resistant to carbapenem. cepA and cfiA alleles were detected by polymerase chain reaction in 240 (87.0%) and 11 (4.0%) of the isolates, respectively. Insertion sequence (IS) elements were found only in the 3 carbapenem-resistant B. fragilis isolates, which produced metallo-β-lactamase at a level detectable by UV spectrophotometry. Sequence analysis showed 1 new cfiA variant, cfiA11, and 2 novel IS elements. The cfiA11 gene revealed 5 amino acid substitutions compared to cfiA, with 97.6% amino acid identity. The transposase, terminal inverted repeat sequence, and target site duplication sequence of the 2 novel IS elements were unique. This study reconfirmed the correlation between ISs and carbapenem resistance in B. fragilis.
AB - Of 276 nonduplicate Bacteroides fragilis clinical isolates recovered from 1997 to 2004, 3 were resistant to carbapenem. cepA and cfiA alleles were detected by polymerase chain reaction in 240 (87.0%) and 11 (4.0%) of the isolates, respectively. Insertion sequence (IS) elements were found only in the 3 carbapenem-resistant B. fragilis isolates, which produced metallo-β-lactamase at a level detectable by UV spectrophotometry. Sequence analysis showed 1 new cfiA variant, cfiA11, and 2 novel IS elements. The cfiA11 gene revealed 5 amino acid substitutions compared to cfiA, with 97.6% amino acid identity. The transposase, terminal inverted repeat sequence, and target site duplication sequence of the 2 novel IS elements were unique. This study reconfirmed the correlation between ISs and carbapenem resistance in B. fragilis.
UR - http://www.scopus.com/inward/record.url?scp=77649289957&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=77649289957&partnerID=8YFLogxK
U2 - 10.1016/j.diagmicrobio.2009.11.003
DO - 10.1016/j.diagmicrobio.2009.11.003
M3 - Article
C2 - 20226324
AN - SCOPUS:77649289957
VL - 66
SP - 343
EP - 348
JO - Diagnostic Microbiology and Infectious Disease
JF - Diagnostic Microbiology and Infectious Disease
SN - 0732-8893
IS - 4
ER -