New cfiA variant and novel insertion sequence elements in carbapenem-resistant Bacteroides fragilis isolates from Korea

Kyoung Ho Roh; Sinyoung Kim; Chang Ki Kim; Jong Hwa Yum; Myung Sook Kim; Dongeun Yong; Seok Hoon Jeong; Kyungwon Lee; June Myung Kim; Yunsop Chong

doi:10.1016/j.diagmicrobio.2009.11.003

New cfiA variant and novel insertion sequence elements in carbapenem-resistant Bacteroides fragilis isolates from Korea

Kyoung Ho Roh, Sinyoung Kim, Chang Ki Kim, Jong Hwa Yum, Myung Sook Kim, Dongeun Yong, Seok Hoon Jeong, Kyungwon Lee, June Myung Kim, Yunsop Chong

Department of Laboratory Medicine

Research output: Contribution to journal › Article › peer-review

23 Citations (Scopus)

Abstract

Of 276 nonduplicate Bacteroides fragilis clinical isolates recovered from 1997 to 2004, 3 were resistant to carbapenem. cepA and cfiA alleles were detected by polymerase chain reaction in 240 (87.0%) and 11 (4.0%) of the isolates, respectively. Insertion sequence (IS) elements were found only in the 3 carbapenem-resistant B. fragilis isolates, which produced metallo-β-lactamase at a level detectable by UV spectrophotometry. Sequence analysis showed 1 new cfiA variant, cfiA₁₁, and 2 novel IS elements. The cfiA₁₁ gene revealed 5 amino acid substitutions compared to cfiA, with 97.6% amino acid identity. The transposase, terminal inverted repeat sequence, and target site duplication sequence of the 2 novel IS elements were unique. This study reconfirmed the correlation between ISs and carbapenem resistance in B. fragilis.

Original language	English
Pages (from-to)	343-348
Number of pages	6
Journal	Diagnostic Microbiology and Infectious Disease
Volume	66
Issue number	4
DOIs	https://doi.org/10.1016/j.diagmicrobio.2009.11.003
Publication status	Published - 2010 Apr

Bibliographical note

Funding Information:
This work was supported by the Brain Korea 21 Project in 2005.

All Science Journal Classification (ASJC) codes

Microbiology (medical)
Infectious Diseases

UN SDGs

This output contributes to the following Sustainable Development Goal(s)

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10.1016/j.diagmicrobio.2009.11.003

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Roh, Kyoung Ho ; Kim, Sinyoung ; Kim, Chang Ki ; Yum, Jong Hwa ; Kim, Myung Sook ; Yong, Dongeun ; Jeong, Seok Hoon ; Lee, Kyungwon ; Kim, June Myung ; Chong, Yunsop. / New cfiA variant and novel insertion sequence elements in carbapenem-resistant Bacteroides fragilis isolates from Korea. In: Diagnostic Microbiology and Infectious Disease. 2010 ; Vol. 66, No. 4. pp. 343-348.

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title = "New cfiA variant and novel insertion sequence elements in carbapenem-resistant Bacteroides fragilis isolates from Korea",

abstract = "Of 276 nonduplicate Bacteroides fragilis clinical isolates recovered from 1997 to 2004, 3 were resistant to carbapenem. cepA and cfiA alleles were detected by polymerase chain reaction in 240 (87.0%) and 11 (4.0%) of the isolates, respectively. Insertion sequence (IS) elements were found only in the 3 carbapenem-resistant B. fragilis isolates, which produced metallo-β-lactamase at a level detectable by UV spectrophotometry. Sequence analysis showed 1 new cfiA variant, cfiA11, and 2 novel IS elements. The cfiA11 gene revealed 5 amino acid substitutions compared to cfiA, with 97.6% amino acid identity. The transposase, terminal inverted repeat sequence, and target site duplication sequence of the 2 novel IS elements were unique. This study reconfirmed the correlation between ISs and carbapenem resistance in B. fragilis.",

author = "Roh, {Kyoung Ho} and Sinyoung Kim and Kim, {Chang Ki} and Yum, {Jong Hwa} and Kim, {Myung Sook} and Dongeun Yong and Jeong, {Seok Hoon} and Kyungwon Lee and Kim, {June Myung} and Yunsop Chong",

note = "Funding Information: This work was supported by the Brain Korea 21 Project in 2005. ",

year = "2010",

month = apr,

doi = "10.1016/j.diagmicrobio.2009.11.003",

language = "English",

volume = "66",

pages = "343--348",

journal = "Diagnostic Microbiology and Infectious Disease",

issn = "0732-8893",

publisher = "Elsevier Inc.",

number = "4",

}

New cfiA variant and novel insertion sequence elements in carbapenem-resistant Bacteroides fragilis isolates from Korea. / Roh, Kyoung Ho; Kim, Sinyoung; Kim, Chang Ki; Yum, Jong Hwa; Kim, Myung Sook; Yong, Dongeun ; Jeong, Seok Hoon ; Lee, Kyungwon; Kim, June Myung; Chong, Yunsop.

In: Diagnostic Microbiology and Infectious Disease, Vol. 66, No. 4, 04.2010, p. 343-348.

Research output: Contribution to journal › Article › peer-review

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AU - Roh, Kyoung Ho

AU - Kim, Sinyoung

AU - Kim, Chang Ki

AU - Yum, Jong Hwa

AU - Kim, Myung Sook

AU - Yong, Dongeun

AU - Jeong, Seok Hoon

AU - Lee, Kyungwon

AU - Kim, June Myung

AU - Chong, Yunsop

N1 - Funding Information: This work was supported by the Brain Korea 21 Project in 2005.

PY - 2010/4

Y1 - 2010/4

N2 - Of 276 nonduplicate Bacteroides fragilis clinical isolates recovered from 1997 to 2004, 3 were resistant to carbapenem. cepA and cfiA alleles were detected by polymerase chain reaction in 240 (87.0%) and 11 (4.0%) of the isolates, respectively. Insertion sequence (IS) elements were found only in the 3 carbapenem-resistant B. fragilis isolates, which produced metallo-β-lactamase at a level detectable by UV spectrophotometry. Sequence analysis showed 1 new cfiA variant, cfiA11, and 2 novel IS elements. The cfiA11 gene revealed 5 amino acid substitutions compared to cfiA, with 97.6% amino acid identity. The transposase, terminal inverted repeat sequence, and target site duplication sequence of the 2 novel IS elements were unique. This study reconfirmed the correlation between ISs and carbapenem resistance in B. fragilis.

AB - Of 276 nonduplicate Bacteroides fragilis clinical isolates recovered from 1997 to 2004, 3 were resistant to carbapenem. cepA and cfiA alleles were detected by polymerase chain reaction in 240 (87.0%) and 11 (4.0%) of the isolates, respectively. Insertion sequence (IS) elements were found only in the 3 carbapenem-resistant B. fragilis isolates, which produced metallo-β-lactamase at a level detectable by UV spectrophotometry. Sequence analysis showed 1 new cfiA variant, cfiA11, and 2 novel IS elements. The cfiA11 gene revealed 5 amino acid substitutions compared to cfiA, with 97.6% amino acid identity. The transposase, terminal inverted repeat sequence, and target site duplication sequence of the 2 novel IS elements were unique. This study reconfirmed the correlation between ISs and carbapenem resistance in B. fragilis.

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New cfiA variant and novel insertion sequence elements in carbapenem-resistant Bacteroides fragilis isolates from Korea

Abstract

Bibliographical note

All Science Journal Classification (ASJC) codes

UN SDGs

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