New cfiA variant and novel insertion sequence elements in carbapenem-resistant Bacteroides fragilis isolates from Korea

Kyoung Ho Roh, Sinyoung Kim, Chang Ki Kim, Jong Hwa Yum, Myung Sook Kim, Dongeun Yong, Seok Hoon Jeong, Kyungwon Lee, June Myung Kim, Yunsop Chong

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Of 276 nonduplicate Bacteroides fragilis clinical isolates recovered from 1997 to 2004, 3 were resistant to carbapenem. cepA and cfiA alleles were detected by polymerase chain reaction in 240 (87.0%) and 11 (4.0%) of the isolates, respectively. Insertion sequence (IS) elements were found only in the 3 carbapenem-resistant B. fragilis isolates, which produced metallo-β-lactamase at a level detectable by UV spectrophotometry. Sequence analysis showed 1 new cfiA variant, cfiA11, and 2 novel IS elements. The cfiA11 gene revealed 5 amino acid substitutions compared to cfiA, with 97.6% amino acid identity. The transposase, terminal inverted repeat sequence, and target site duplication sequence of the 2 novel IS elements were unique. This study reconfirmed the correlation between ISs and carbapenem resistance in B. fragilis.

Original languageEnglish
Pages (from-to)343-348
Number of pages6
JournalDiagnostic Microbiology and Infectious Disease
Issue number4
Publication statusPublished - 2010 Apr 1


All Science Journal Classification (ASJC) codes

  • Microbiology (medical)
  • Infectious Diseases

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