New cfiA variant and novel insertion sequence elements in carbapenem-resistant Bacteroides fragilis isolates from Korea

Kyoung Ho Roh, Sinyoung Kim, Chang Ki Kim, Jong Hwa Yum, Myung Sook Kim, Dongeun Yong, Seok Hoon Jeong, Kyungwon Lee, June Myung Kim, Yunsop Chong

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Of 276 nonduplicate Bacteroides fragilis clinical isolates recovered from 1997 to 2004, 3 were resistant to carbapenem. cepA and cfiA alleles were detected by polymerase chain reaction in 240 (87.0%) and 11 (4.0%) of the isolates, respectively. Insertion sequence (IS) elements were found only in the 3 carbapenem-resistant B. fragilis isolates, which produced metallo-β-lactamase at a level detectable by UV spectrophotometry. Sequence analysis showed 1 new cfiA variant, cfiA11, and 2 novel IS elements. The cfiA11 gene revealed 5 amino acid substitutions compared to cfiA, with 97.6% amino acid identity. The transposase, terminal inverted repeat sequence, and target site duplication sequence of the 2 novel IS elements were unique. This study reconfirmed the correlation between ISs and carbapenem resistance in B. fragilis.

Original languageEnglish
Pages (from-to)343-348
Number of pages6
JournalDiagnostic Microbiology and Infectious Disease
Volume66
Issue number4
DOIs
Publication statusPublished - 2010 Apr 1

Fingerprint

Bacteroides fragilis
Carbapenems
DNA Transposable Elements
Korea
Inverted Repeat Sequences
Transposases
Terminal Repeat Sequences
Spectrophotometry
Amino Acid Substitution
Sequence Analysis
Alleles
Amino Acids
Polymerase Chain Reaction
Genes

All Science Journal Classification (ASJC) codes

  • Microbiology (medical)
  • Infectious Diseases

Cite this

Roh, Kyoung Ho ; Kim, Sinyoung ; Kim, Chang Ki ; Yum, Jong Hwa ; Kim, Myung Sook ; Yong, Dongeun ; Jeong, Seok Hoon ; Lee, Kyungwon ; Kim, June Myung ; Chong, Yunsop. / New cfiA variant and novel insertion sequence elements in carbapenem-resistant Bacteroides fragilis isolates from Korea. In: Diagnostic Microbiology and Infectious Disease. 2010 ; Vol. 66, No. 4. pp. 343-348.
@article{228daebc219f4c5fbbc6f5c2645cc73e,
title = "New cfiA variant and novel insertion sequence elements in carbapenem-resistant Bacteroides fragilis isolates from Korea",
abstract = "Of 276 nonduplicate Bacteroides fragilis clinical isolates recovered from 1997 to 2004, 3 were resistant to carbapenem. cepA and cfiA alleles were detected by polymerase chain reaction in 240 (87.0{\%}) and 11 (4.0{\%}) of the isolates, respectively. Insertion sequence (IS) elements were found only in the 3 carbapenem-resistant B. fragilis isolates, which produced metallo-β-lactamase at a level detectable by UV spectrophotometry. Sequence analysis showed 1 new cfiA variant, cfiA11, and 2 novel IS elements. The cfiA11 gene revealed 5 amino acid substitutions compared to cfiA, with 97.6{\%} amino acid identity. The transposase, terminal inverted repeat sequence, and target site duplication sequence of the 2 novel IS elements were unique. This study reconfirmed the correlation between ISs and carbapenem resistance in B. fragilis.",
author = "Roh, {Kyoung Ho} and Sinyoung Kim and Kim, {Chang Ki} and Yum, {Jong Hwa} and Kim, {Myung Sook} and Dongeun Yong and Jeong, {Seok Hoon} and Kyungwon Lee and Kim, {June Myung} and Yunsop Chong",
year = "2010",
month = "4",
day = "1",
doi = "10.1016/j.diagmicrobio.2009.11.003",
language = "English",
volume = "66",
pages = "343--348",
journal = "Diagnostic Microbiology and Infectious Disease",
issn = "0732-8893",
publisher = "Elsevier Inc.",
number = "4",

}

New cfiA variant and novel insertion sequence elements in carbapenem-resistant Bacteroides fragilis isolates from Korea. / Roh, Kyoung Ho; Kim, Sinyoung; Kim, Chang Ki; Yum, Jong Hwa; Kim, Myung Sook; Yong, Dongeun; Jeong, Seok Hoon; Lee, Kyungwon; Kim, June Myung; Chong, Yunsop.

In: Diagnostic Microbiology and Infectious Disease, Vol. 66, No. 4, 01.04.2010, p. 343-348.

Research output: Contribution to journalArticle

TY - JOUR

T1 - New cfiA variant and novel insertion sequence elements in carbapenem-resistant Bacteroides fragilis isolates from Korea

AU - Roh, Kyoung Ho

AU - Kim, Sinyoung

AU - Kim, Chang Ki

AU - Yum, Jong Hwa

AU - Kim, Myung Sook

AU - Yong, Dongeun

AU - Jeong, Seok Hoon

AU - Lee, Kyungwon

AU - Kim, June Myung

AU - Chong, Yunsop

PY - 2010/4/1

Y1 - 2010/4/1

N2 - Of 276 nonduplicate Bacteroides fragilis clinical isolates recovered from 1997 to 2004, 3 were resistant to carbapenem. cepA and cfiA alleles were detected by polymerase chain reaction in 240 (87.0%) and 11 (4.0%) of the isolates, respectively. Insertion sequence (IS) elements were found only in the 3 carbapenem-resistant B. fragilis isolates, which produced metallo-β-lactamase at a level detectable by UV spectrophotometry. Sequence analysis showed 1 new cfiA variant, cfiA11, and 2 novel IS elements. The cfiA11 gene revealed 5 amino acid substitutions compared to cfiA, with 97.6% amino acid identity. The transposase, terminal inverted repeat sequence, and target site duplication sequence of the 2 novel IS elements were unique. This study reconfirmed the correlation between ISs and carbapenem resistance in B. fragilis.

AB - Of 276 nonduplicate Bacteroides fragilis clinical isolates recovered from 1997 to 2004, 3 were resistant to carbapenem. cepA and cfiA alleles were detected by polymerase chain reaction in 240 (87.0%) and 11 (4.0%) of the isolates, respectively. Insertion sequence (IS) elements were found only in the 3 carbapenem-resistant B. fragilis isolates, which produced metallo-β-lactamase at a level detectable by UV spectrophotometry. Sequence analysis showed 1 new cfiA variant, cfiA11, and 2 novel IS elements. The cfiA11 gene revealed 5 amino acid substitutions compared to cfiA, with 97.6% amino acid identity. The transposase, terminal inverted repeat sequence, and target site duplication sequence of the 2 novel IS elements were unique. This study reconfirmed the correlation between ISs and carbapenem resistance in B. fragilis.

UR - http://www.scopus.com/inward/record.url?scp=77649289957&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77649289957&partnerID=8YFLogxK

U2 - 10.1016/j.diagmicrobio.2009.11.003

DO - 10.1016/j.diagmicrobio.2009.11.003

M3 - Article

C2 - 20226324

AN - SCOPUS:77649289957

VL - 66

SP - 343

EP - 348

JO - Diagnostic Microbiology and Infectious Disease

JF - Diagnostic Microbiology and Infectious Disease

SN - 0732-8893

IS - 4

ER -