New Delhi metallo-beta-lactamase-producing enterobacteriaceae in South Korea Between 2010 and 20

Eun Jeong Yoon, Da Young Kang, Ji Woo Yang, Dokyun Kim, Hyukmin Lee, Kwang Jun Lee, Seok Hoon Jeong

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

This study was carried out to investigate the epidemiological time-course of New Delhi metallo-beta-lactamase- (NDM-) mediated carbapenem resistance in Enterobacteriaceae in South Korea. A total of 146 non-duplicate NDM-producing Enterobacteriaceae recovered between 2010 and 2015 were voluntarily collected from 33 general hospitals and confirmed by PCR. The species were identified by sequences of the 16S rDNA. Antimicrobial susceptibility was determined either by the disk diffusion method or by broth microdilution, and the carbapenem MICs were determined by agar dilution. Then, multilocus sequence typing and PCR-based replicon typing was carried out. Co-carried genes for drug resistance were identified by PCR and sequencing. The entire genomes of eight random selected NDM producers were sequenced. A total of 69 Klebsiella pneumoniae of 12 sequence types (STs), 34 Escherichia coli of 15 STs, 28 Enterobacter spp. (including one Enterobacter aerogenes), nine Citrobacter freundii, four Raoultella spp., and two Klebsiella oxytoca isolates produced either NDM-1 (n = 126), NDM-5 (n = 18), or NDM-7 (n = 2). The isolates co-produced CTX-M-type ESBL (52.1%), AmpCs (27.4%), additional carbapenemases (7.1%), and/or 16S rRNA methyltransferases (4.8%), resulting in multidrug-resistance (47.9%) or extensively drug-resistance (52.1%). Among plasmids harboring blaNDM, IncX3 was predominant (77.4%), followed by the IncFII type (5.8%). Genome analysis revealed inter-species and inter-strain horizontal gene transfer of the plasmid. Both clonal dissemination and plasmid transfer contributed to the wide dissemination of NDM producers in South Korea.

Original languageEnglish
Number of pages1
JournalFrontiers in Microbiology
Volume9
Issue numberMAR
DOIs
Publication statusPublished - 2018 Mar 29

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Republic of Korea
Enterobacteriaceae
Carbapenems
Plasmids
Drug Resistance
Polymerase Chain Reaction
Klebsiella oxytoca
Genome
Citrobacter freundii
Enterobacter aerogenes
Multilocus Sequence Typing
Horizontal Gene Transfer
Enterobacter
Replicon
Methyltransferases
Klebsiella pneumoniae
Multiple Drug Resistance
Ribosomal DNA
General Hospitals
Agar

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Microbiology (medical)

Cite this

Yoon, Eun Jeong ; Kang, Da Young ; Yang, Ji Woo ; Kim, Dokyun ; Lee, Hyukmin ; Lee, Kwang Jun ; Jeong, Seok Hoon. / New Delhi metallo-beta-lactamase-producing enterobacteriaceae in South Korea Between 2010 and 20. In: Frontiers in Microbiology. 2018 ; Vol. 9, No. MAR.
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abstract = "This study was carried out to investigate the epidemiological time-course of New Delhi metallo-beta-lactamase- (NDM-) mediated carbapenem resistance in Enterobacteriaceae in South Korea. A total of 146 non-duplicate NDM-producing Enterobacteriaceae recovered between 2010 and 2015 were voluntarily collected from 33 general hospitals and confirmed by PCR. The species were identified by sequences of the 16S rDNA. Antimicrobial susceptibility was determined either by the disk diffusion method or by broth microdilution, and the carbapenem MICs were determined by agar dilution. Then, multilocus sequence typing and PCR-based replicon typing was carried out. Co-carried genes for drug resistance were identified by PCR and sequencing. The entire genomes of eight random selected NDM producers were sequenced. A total of 69 Klebsiella pneumoniae of 12 sequence types (STs), 34 Escherichia coli of 15 STs, 28 Enterobacter spp. (including one Enterobacter aerogenes), nine Citrobacter freundii, four Raoultella spp., and two Klebsiella oxytoca isolates produced either NDM-1 (n = 126), NDM-5 (n = 18), or NDM-7 (n = 2). The isolates co-produced CTX-M-type ESBL (52.1{\%}), AmpCs (27.4{\%}), additional carbapenemases (7.1{\%}), and/or 16S rRNA methyltransferases (4.8{\%}), resulting in multidrug-resistance (47.9{\%}) or extensively drug-resistance (52.1{\%}). Among plasmids harboring blaNDM, IncX3 was predominant (77.4{\%}), followed by the IncFII type (5.8{\%}). Genome analysis revealed inter-species and inter-strain horizontal gene transfer of the plasmid. Both clonal dissemination and plasmid transfer contributed to the wide dissemination of NDM producers in South Korea.",
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New Delhi metallo-beta-lactamase-producing enterobacteriaceae in South Korea Between 2010 and 20. / Yoon, Eun Jeong; Kang, Da Young; Yang, Ji Woo; Kim, Dokyun; Lee, Hyukmin; Lee, Kwang Jun; Jeong, Seok Hoon.

In: Frontiers in Microbiology, Vol. 9, No. MAR, 29.03.2018.

Research output: Contribution to journalArticle

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AB - This study was carried out to investigate the epidemiological time-course of New Delhi metallo-beta-lactamase- (NDM-) mediated carbapenem resistance in Enterobacteriaceae in South Korea. A total of 146 non-duplicate NDM-producing Enterobacteriaceae recovered between 2010 and 2015 were voluntarily collected from 33 general hospitals and confirmed by PCR. The species were identified by sequences of the 16S rDNA. Antimicrobial susceptibility was determined either by the disk diffusion method or by broth microdilution, and the carbapenem MICs were determined by agar dilution. Then, multilocus sequence typing and PCR-based replicon typing was carried out. Co-carried genes for drug resistance were identified by PCR and sequencing. The entire genomes of eight random selected NDM producers were sequenced. A total of 69 Klebsiella pneumoniae of 12 sequence types (STs), 34 Escherichia coli of 15 STs, 28 Enterobacter spp. (including one Enterobacter aerogenes), nine Citrobacter freundii, four Raoultella spp., and two Klebsiella oxytoca isolates produced either NDM-1 (n = 126), NDM-5 (n = 18), or NDM-7 (n = 2). The isolates co-produced CTX-M-type ESBL (52.1%), AmpCs (27.4%), additional carbapenemases (7.1%), and/or 16S rRNA methyltransferases (4.8%), resulting in multidrug-resistance (47.9%) or extensively drug-resistance (52.1%). Among plasmids harboring blaNDM, IncX3 was predominant (77.4%), followed by the IncFII type (5.8%). Genome analysis revealed inter-species and inter-strain horizontal gene transfer of the plasmid. Both clonal dissemination and plasmid transfer contributed to the wide dissemination of NDM producers in South Korea.

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