New GABAergic Neurogenesis in the Hippocampal CA1 Region of a Gerbil Model of Long-Term Survival after Transient Cerebral Ischemic Injury

Jae Chul Lee, Joon Ha Park, Ji Hyeon Ahn, In Hye Kim, Jeong Hwi Cho, Jung Hoon Choi, Ki Yeon Yoo, Choong Hyun Lee, In Koo Hwang, Jun Hwi Cho, Young Guen Kwon, Young Myeong Kim, Il Jun Kang, Moo Ho Won

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23 Citations (Scopus)

Abstract

We investigated the probability of newly generated neurons that could survive and mature in the ischemic hippocampal CA1 region (CA1) of a gerbil model of transient cerebral ischemia. Neuronal death was shown in the stratum pyramidale (SP) from 4 days post-ischemia, and a significant increase in NeuN-positive (+) neurons was found in the SP at 180 days post-ischemia. 5-Bromo-2-deoxyuridine (BrdU)+ cells were co-stained with NeuN and glutamic decarboxylase 67 (GAD67). Brain-derived neurotrophic factor (BDNF) immunoreactivity and protein level was shown in nonpyramidal cells from 4 days post-ischemia, and the immunoreactivity was strong at 30 days post-ischemia and not significantly changed until 180 days post-ischemia. Furthermore, TrkB immunoreactivity was co-stained with GAD67 when we examined at 180 days post-ischemia. Myelin basic protein (MBP)+ nerve fibers were reduced at 4 days post-ischemia and maintained until 60 days post-ischemia, and MBP immunoreactivity and levels were significantly increased at 180 days post-ischemia. In the passive avoidance test, cognitive dysfunction was improved at 180 days post-ischemia. These results suggest that the differentiation of neural progenitor cells into new GABAergic neurons may be promoted via BDNF in the ischemic CA1 and that the neurogenesis may partially mediate the recovery of cognitive impairments via increasing myelinated nerve fibers.

Original languageEnglish
Pages (from-to)581-592
Number of pages12
JournalBrain Pathology
Volume26
Issue number5
DOIs
Publication statusPublished - 2016 Sep 1

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine
  • Neuroscience(all)
  • Clinical Neurology

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    Lee, J. C., Park, J. H., Ahn, J. H., Kim, I. H., Cho, J. H., Choi, J. H., Yoo, K. Y., Lee, C. H., Hwang, I. K., Cho, J. H., Kwon, Y. G., Kim, Y. M., Kang, I. J., & Won, M. H. (2016). New GABAergic Neurogenesis in the Hippocampal CA1 Region of a Gerbil Model of Long-Term Survival after Transient Cerebral Ischemic Injury. Brain Pathology, 26(5), 581-592. https://doi.org/10.1111/bpa.12334