New perspectives for biomedical imaging at depth

We have seen great advances in photonics based methods for imaging and manipulation. However whislt improvements have been seen in beating the diffraction limit and gaining wide field imaging, imaging through scattering media remains a challenge. I will describe approaches that tackle this issue. In particular I will describe using a geometry where the excitation beam is orthogonal to the detection arm of the optical system has come to the fore. This method is known as light sheet imaging or selective plane illumination microscopy. This talk will describe the basic premises of this field and highlight how we may increase penetration using propagation invariant Bessel and Airy beams as well as multiphoton imaging, including particularly three photon excitation. In the second part of my talk I will move to an epi-fluorescent geometry and describe the use of temporal focusing with single pixel detection. This scheme which we term TRAFIX can image through turbid media without prior knowledge of the scattering properties present and can be implemented on both two and three photon imaging.