Newly developed method for mouse olfactory behavior tests using an automatic video tracking system

Hyung Ju Cho, Yong Hyuk Lee, Bo Ra Kim, Hong Kyung Kim, Hyo Jin Chung, Sang Chul Park, Je Kyung Seong, Joo Heon Yoon, Chang-Hoon Kim

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Objective The mouse is the most popular animal model in olfactory research. Behavior tests with odorants are essential for determining olfactory phenotype. To the best of our knowledge, the mouse olfactory behavior test has not been standardized, making the results vulnerable to inter-observer variation. We sought to develop a new mouse olfactory behavior test assessed by an automatic video tracking system with minimal inter-observer variation. Methods A video-tracking system was used to automatically track mouse behavior in standard breeding cages with C57BL/6N mice. We tested two odorants (peanut butter for the preference test, 2MB acid for the avoidance test) and distilled water (for a control). Mouse behavior was recorded for 3 min and analyzed. For the preference test, investigation time was measured. For the avoidance test, time spent in sectors away from the odorant zone was measured. To confirm our experimental settings, we also evaluated an anosmia mouse model prepared with intranasal administration of ZnSO 4 . Results All strains of mice showed reproducible behavior patterns of preference or avoidance for the odorants. The anosmia mouse model, as expected, failed to show an olfactory ability for preference or avoidance, and this was well-matched by histologic changes caused by the ZnSO 4 treatment. The automatic video tracking system successfully tracked and automatically calculated mouse behavior with good reproducibility. Conclusion Our olfactory behavior test offers a simple and accurate method to evaluate olfactory function in mice. This test can be utilized as a possible standard method to search for features of olfactory phenotypes in mice.

Original languageEnglish
Pages (from-to)103-110
Number of pages8
JournalAuris Nasus Larynx
Volume45
Issue number1
DOIs
Publication statusPublished - 2018 Feb 1

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Olfaction Disorders
Observer Variation
Behavior Rating Scale
Phenotype
Intranasal Administration
Butter
Aptitude
Inbred C57BL Mouse
Breeding
Animal Models
Acids
Odorants
Water
Research
Therapeutics
Arachis

All Science Journal Classification (ASJC) codes

  • Surgery
  • Otorhinolaryngology

Cite this

Cho, Hyung Ju ; Lee, Yong Hyuk ; Kim, Bo Ra ; Kim, Hong Kyung ; Chung, Hyo Jin ; Park, Sang Chul ; Seong, Je Kyung ; Yoon, Joo Heon ; Kim, Chang-Hoon. / Newly developed method for mouse olfactory behavior tests using an automatic video tracking system. In: Auris Nasus Larynx. 2018 ; Vol. 45, No. 1. pp. 103-110.
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abstract = "Objective The mouse is the most popular animal model in olfactory research. Behavior tests with odorants are essential for determining olfactory phenotype. To the best of our knowledge, the mouse olfactory behavior test has not been standardized, making the results vulnerable to inter-observer variation. We sought to develop a new mouse olfactory behavior test assessed by an automatic video tracking system with minimal inter-observer variation. Methods A video-tracking system was used to automatically track mouse behavior in standard breeding cages with C57BL/6N mice. We tested two odorants (peanut butter for the preference test, 2MB acid for the avoidance test) and distilled water (for a control). Mouse behavior was recorded for 3 min and analyzed. For the preference test, investigation time was measured. For the avoidance test, time spent in sectors away from the odorant zone was measured. To confirm our experimental settings, we also evaluated an anosmia mouse model prepared with intranasal administration of ZnSO 4 . Results All strains of mice showed reproducible behavior patterns of preference or avoidance for the odorants. The anosmia mouse model, as expected, failed to show an olfactory ability for preference or avoidance, and this was well-matched by histologic changes caused by the ZnSO 4 treatment. The automatic video tracking system successfully tracked and automatically calculated mouse behavior with good reproducibility. Conclusion Our olfactory behavior test offers a simple and accurate method to evaluate olfactory function in mice. This test can be utilized as a possible standard method to search for features of olfactory phenotypes in mice.",
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Cho, HJ, Lee, YH, Kim, BR, Kim, HK, Chung, HJ, Park, SC, Seong, JK, Yoon, JH & Kim, C-H 2018, 'Newly developed method for mouse olfactory behavior tests using an automatic video tracking system', Auris Nasus Larynx, vol. 45, no. 1, pp. 103-110. https://doi.org/10.1016/j.anl.2017.03.007

Newly developed method for mouse olfactory behavior tests using an automatic video tracking system. / Cho, Hyung Ju; Lee, Yong Hyuk; Kim, Bo Ra; Kim, Hong Kyung; Chung, Hyo Jin; Park, Sang Chul; Seong, Je Kyung; Yoon, Joo Heon; Kim, Chang-Hoon.

In: Auris Nasus Larynx, Vol. 45, No. 1, 01.02.2018, p. 103-110.

Research output: Contribution to journalArticle

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N2 - Objective The mouse is the most popular animal model in olfactory research. Behavior tests with odorants are essential for determining olfactory phenotype. To the best of our knowledge, the mouse olfactory behavior test has not been standardized, making the results vulnerable to inter-observer variation. We sought to develop a new mouse olfactory behavior test assessed by an automatic video tracking system with minimal inter-observer variation. Methods A video-tracking system was used to automatically track mouse behavior in standard breeding cages with C57BL/6N mice. We tested two odorants (peanut butter for the preference test, 2MB acid for the avoidance test) and distilled water (for a control). Mouse behavior was recorded for 3 min and analyzed. For the preference test, investigation time was measured. For the avoidance test, time spent in sectors away from the odorant zone was measured. To confirm our experimental settings, we also evaluated an anosmia mouse model prepared with intranasal administration of ZnSO 4 . Results All strains of mice showed reproducible behavior patterns of preference or avoidance for the odorants. The anosmia mouse model, as expected, failed to show an olfactory ability for preference or avoidance, and this was well-matched by histologic changes caused by the ZnSO 4 treatment. The automatic video tracking system successfully tracked and automatically calculated mouse behavior with good reproducibility. Conclusion Our olfactory behavior test offers a simple and accurate method to evaluate olfactory function in mice. This test can be utilized as a possible standard method to search for features of olfactory phenotypes in mice.

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