Newly developed method for mouse olfactory behavior tests using an automatic video tracking system

Hyung Ju Cho, Yong Hyuk Lee, Bo Ra Kim, Hong Kyung Kim, Hyo Jin Chung, Sang Chul Park, Je Kyung Seong, Joo Heon Yoon, Chang Hoon Kim

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)

Abstract

Objective The mouse is the most popular animal model in olfactory research. Behavior tests with odorants are essential for determining olfactory phenotype. To the best of our knowledge, the mouse olfactory behavior test has not been standardized, making the results vulnerable to inter-observer variation. We sought to develop a new mouse olfactory behavior test assessed by an automatic video tracking system with minimal inter-observer variation. Methods A video-tracking system was used to automatically track mouse behavior in standard breeding cages with C57BL/6N mice. We tested two odorants (peanut butter for the preference test, 2MB acid for the avoidance test) and distilled water (for a control). Mouse behavior was recorded for 3 min and analyzed. For the preference test, investigation time was measured. For the avoidance test, time spent in sectors away from the odorant zone was measured. To confirm our experimental settings, we also evaluated an anosmia mouse model prepared with intranasal administration of ZnSO4. Results All strains of mice showed reproducible behavior patterns of preference or avoidance for the odorants. The anosmia mouse model, as expected, failed to show an olfactory ability for preference or avoidance, and this was well-matched by histologic changes caused by the ZnSO4 treatment. The automatic video tracking system successfully tracked and automatically calculated mouse behavior with good reproducibility. Conclusion Our olfactory behavior test offers a simple and accurate method to evaluate olfactory function in mice. This test can be utilized as a possible standard method to search for features of olfactory phenotypes in mice.

Original languageEnglish
Pages (from-to)103-110
Number of pages8
JournalAuris Nasus Larynx
Volume45
Issue number1
DOIs
Publication statusPublished - 2018 Feb

Bibliographical note

Funding Information:
This research was supported by the Bio & Medical Technology Development Program of the National Research Foundation (NRF) funded by the ministry of Science, ICT & Future Planning (NRF-2016M3A9D5A01952414). This work was supported by the National Research Foundation of Korea (NRF) Grant funded by the Korean Government (MSIP) (No. 2016R1A5A2008630). This study was supported by a faculty research grant of Yonsei University College of Medicine for (6-2011-0122).

Funding Information:
This research was supported by the Bio & Medical Technology Development Program of the National Research Foundation (NRF) funded by the ministry of Science, ICT & Future Planning (NRF-2016M3A9D5A01952414). This work was supported by the National Research Foundation of Korea (NRF) Grant funded by the Korean Government (MSIP) (No. 2016R1A5A2008630 ). This study was supported by a faculty research grant of Yonsei University College of Medicine for ( 6-2011-0122 ).

Publisher Copyright:
© 2017

All Science Journal Classification (ASJC) codes

  • Surgery
  • Otorhinolaryngology

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