Nitric oxide-mediated endothelium-dependent relaxation of rat thoracic aorta induced by aqueous extract of red rice fermented with Monascus ruber

Mee Ra Rhyu, Duk Kyung Kim, Hye Young Kim, Bo Kyung Kim

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Vasodilatory effects of aqueous extract of red rice fermented with Monascus ruber IFO32318 were examined on the isolated rat aorta. The water phase of fermented rice with Monascus (WP/FRM, 0.1-10 mg/ml) caused a transient relaxation of the endothelium-intact rat aorta precontracted with norepinephrine (NE, 300 nM). The WP/FRM-induced relaxation was abolished by removal of endothelium or in the presence of N(G)-nitro-L-arginine (L-NNA, 10 μM), a nitric oxide (NO) synthase inhibitor. Neither atropine, a muscarinic receptor antagonist (10 μM), nor indomethacin, a cyclooxygenase inhibitor (10 μM), altered the WP/FRM-induced endothelium-dependent relaxation. γ-Aminobutyric acid (GABA), one of the principle components of the extract, did not affect the muscle tension of the aorta with intact endothelium. In addition, WP/FRM increased the production of NO in primary cultured endothelial cells from human umbilical vein. The enhanced production of NO by WP/FRM was diminished by pretreatment with L-NNA (10 μM). In conclusion, WP/FRM induces relaxation of rat aorta by releasing NO from endothelium. There seem to be some unknown factor(s) other than acetylcholine (Ach) and GABA, in the aqueous extract of red rice, which stimulate vascular endothelial cells to produce and/or release NO leading to endothelium-dependent relaxation by WP/FRM. Copyright (C) 2000 Elsevier Science Ireland Ltd.

Original languageEnglish
Pages (from-to)29-34
Number of pages6
JournalJournal of Ethnopharmacology
Volume70
Issue number1
DOIs
Publication statusPublished - 2000 Apr 1

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Monascus
Thoracic Aorta
Endothelium
Nitric Oxide
Aorta
gamma-Aminobutyric Acid
Endothelial Cells
Aminobutyrates
Muscle Tonus
Umbilical Veins
Muscarinic Antagonists
Cyclooxygenase Inhibitors
Muscarinic Receptors
Atropine
Nitric Oxide Synthase
Indomethacin
Acetylcholine
Arginine
Oryza
Cultured Cells

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Drug Discovery

Cite this

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title = "Nitric oxide-mediated endothelium-dependent relaxation of rat thoracic aorta induced by aqueous extract of red rice fermented with Monascus ruber",
abstract = "Vasodilatory effects of aqueous extract of red rice fermented with Monascus ruber IFO32318 were examined on the isolated rat aorta. The water phase of fermented rice with Monascus (WP/FRM, 0.1-10 mg/ml) caused a transient relaxation of the endothelium-intact rat aorta precontracted with norepinephrine (NE, 300 nM). The WP/FRM-induced relaxation was abolished by removal of endothelium or in the presence of N(G)-nitro-L-arginine (L-NNA, 10 μM), a nitric oxide (NO) synthase inhibitor. Neither atropine, a muscarinic receptor antagonist (10 μM), nor indomethacin, a cyclooxygenase inhibitor (10 μM), altered the WP/FRM-induced endothelium-dependent relaxation. γ-Aminobutyric acid (GABA), one of the principle components of the extract, did not affect the muscle tension of the aorta with intact endothelium. In addition, WP/FRM increased the production of NO in primary cultured endothelial cells from human umbilical vein. The enhanced production of NO by WP/FRM was diminished by pretreatment with L-NNA (10 μM). In conclusion, WP/FRM induces relaxation of rat aorta by releasing NO from endothelium. There seem to be some unknown factor(s) other than acetylcholine (Ach) and GABA, in the aqueous extract of red rice, which stimulate vascular endothelial cells to produce and/or release NO leading to endothelium-dependent relaxation by WP/FRM. Copyright (C) 2000 Elsevier Science Ireland Ltd.",
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Nitric oxide-mediated endothelium-dependent relaxation of rat thoracic aorta induced by aqueous extract of red rice fermented with Monascus ruber. / Rhyu, Mee Ra; Kim, Duk Kyung; Kim, Hye Young; Kim, Bo Kyung.

In: Journal of Ethnopharmacology, Vol. 70, No. 1, 01.04.2000, p. 29-34.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Nitric oxide-mediated endothelium-dependent relaxation of rat thoracic aorta induced by aqueous extract of red rice fermented with Monascus ruber

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N2 - Vasodilatory effects of aqueous extract of red rice fermented with Monascus ruber IFO32318 were examined on the isolated rat aorta. The water phase of fermented rice with Monascus (WP/FRM, 0.1-10 mg/ml) caused a transient relaxation of the endothelium-intact rat aorta precontracted with norepinephrine (NE, 300 nM). The WP/FRM-induced relaxation was abolished by removal of endothelium or in the presence of N(G)-nitro-L-arginine (L-NNA, 10 μM), a nitric oxide (NO) synthase inhibitor. Neither atropine, a muscarinic receptor antagonist (10 μM), nor indomethacin, a cyclooxygenase inhibitor (10 μM), altered the WP/FRM-induced endothelium-dependent relaxation. γ-Aminobutyric acid (GABA), one of the principle components of the extract, did not affect the muscle tension of the aorta with intact endothelium. In addition, WP/FRM increased the production of NO in primary cultured endothelial cells from human umbilical vein. The enhanced production of NO by WP/FRM was diminished by pretreatment with L-NNA (10 μM). In conclusion, WP/FRM induces relaxation of rat aorta by releasing NO from endothelium. There seem to be some unknown factor(s) other than acetylcholine (Ach) and GABA, in the aqueous extract of red rice, which stimulate vascular endothelial cells to produce and/or release NO leading to endothelium-dependent relaxation by WP/FRM. Copyright (C) 2000 Elsevier Science Ireland Ltd.

AB - Vasodilatory effects of aqueous extract of red rice fermented with Monascus ruber IFO32318 were examined on the isolated rat aorta. The water phase of fermented rice with Monascus (WP/FRM, 0.1-10 mg/ml) caused a transient relaxation of the endothelium-intact rat aorta precontracted with norepinephrine (NE, 300 nM). The WP/FRM-induced relaxation was abolished by removal of endothelium or in the presence of N(G)-nitro-L-arginine (L-NNA, 10 μM), a nitric oxide (NO) synthase inhibitor. Neither atropine, a muscarinic receptor antagonist (10 μM), nor indomethacin, a cyclooxygenase inhibitor (10 μM), altered the WP/FRM-induced endothelium-dependent relaxation. γ-Aminobutyric acid (GABA), one of the principle components of the extract, did not affect the muscle tension of the aorta with intact endothelium. In addition, WP/FRM increased the production of NO in primary cultured endothelial cells from human umbilical vein. The enhanced production of NO by WP/FRM was diminished by pretreatment with L-NNA (10 μM). In conclusion, WP/FRM induces relaxation of rat aorta by releasing NO from endothelium. There seem to be some unknown factor(s) other than acetylcholine (Ach) and GABA, in the aqueous extract of red rice, which stimulate vascular endothelial cells to produce and/or release NO leading to endothelium-dependent relaxation by WP/FRM. Copyright (C) 2000 Elsevier Science Ireland Ltd.

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