Nocardioides daeguensis sp. nov., a nitratereducing bacterium isolated from activated sludge of an industrial wastewater treatment plant

Yingshun Cui, Sung Geun Woo, Jangho Lee, Sahastranshu Sinha, Myung Suk Kang, Long Jin, Kwang Kyu Kim, Joonhong Park, Myungjin Lee, Sung Taik Lee

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

A Gram-reaction-positive, rod-shaped, non-spore-forming bacterium (strain 2C1-5T) was isolated from activated sludge of an industrial wastewater treatment plant in Daegu, South Korea. Its taxonomic position was investigated by using a polyphasic approach. On the basis of 16S rRNA gene sequence similarity, the closest phylogenetic relatives were the type strains of Nocardioides nitrophenolicus (98.6% similarity), N. kongjuensis (98.5 %), N. caeni (98.4 %), N. simplex (98.3 %), N. aromaticivorans (98.1 %) and N. ginsengisoli (97.5 %); the phylogenetic distance from other species with validly published names within the genus Nocardioides was greater than 3%. Strain 2C1-5T was characterized chemotaxonomically as having LL-2, 6-diaminopimelic acid in the cell-wall peptidoglycan, MK-8(H4) as the predominant menaquinone and iso-C16: 0, C16: 0 and C17: 1ω6c as the major fatty acids. The G+C content of the genomic DNA was 74.9 mol%. These chemotaxonomic properties and phenotypic characteristics supported the affiliation of strain 2C1-5T to the genus Nocardioides. The results of physiological and biochemical tests allowed genotypic and phenotypic differentiation of strain 2C1-5T from existing species with validly published names. Therefore, strain 2C1-5T represents a novel species of the genus Nocardioides, for which the name Nocardioides daeguensis sp. nov. is proposed, with the type strain 2C1-5T (=JCM 17460T=KCTC 19799T).

Original languageEnglish
Pages (from-to)3727-3732
Number of pages6
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume63
Issue numberPART10
DOIs
Publication statusPublished - 2013 Oct 1

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Nocardioides
activated sludge
Waste Water
Sewage
Names
Bacteria
bacterium
new species
bacteria
Gram-Positive Rods
Diaminopimelic Acid
phylogenetics
Vitamin K 2
Republic of Korea
Peptidoglycan
Base Composition
rRNA Genes
Cell Wall
genomics
Fatty Acids

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics

Cite this

Cui, Yingshun ; Woo, Sung Geun ; Lee, Jangho ; Sinha, Sahastranshu ; Kang, Myung Suk ; Jin, Long ; Kim, Kwang Kyu ; Park, Joonhong ; Lee, Myungjin ; Lee, Sung Taik. / Nocardioides daeguensis sp. nov., a nitratereducing bacterium isolated from activated sludge of an industrial wastewater treatment plant. In: International Journal of Systematic and Evolutionary Microbiology. 2013 ; Vol. 63, No. PART10. pp. 3727-3732.
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abstract = "A Gram-reaction-positive, rod-shaped, non-spore-forming bacterium (strain 2C1-5T) was isolated from activated sludge of an industrial wastewater treatment plant in Daegu, South Korea. Its taxonomic position was investigated by using a polyphasic approach. On the basis of 16S rRNA gene sequence similarity, the closest phylogenetic relatives were the type strains of Nocardioides nitrophenolicus (98.6{\%} similarity), N. kongjuensis (98.5 {\%}), N. caeni (98.4 {\%}), N. simplex (98.3 {\%}), N. aromaticivorans (98.1 {\%}) and N. ginsengisoli (97.5 {\%}); the phylogenetic distance from other species with validly published names within the genus Nocardioides was greater than 3{\%}. Strain 2C1-5T was characterized chemotaxonomically as having LL-2, 6-diaminopimelic acid in the cell-wall peptidoglycan, MK-8(H4) as the predominant menaquinone and iso-C16: 0, C16: 0 and C17: 1ω6c as the major fatty acids. The G+C content of the genomic DNA was 74.9 mol{\%}. These chemotaxonomic properties and phenotypic characteristics supported the affiliation of strain 2C1-5T to the genus Nocardioides. The results of physiological and biochemical tests allowed genotypic and phenotypic differentiation of strain 2C1-5T from existing species with validly published names. Therefore, strain 2C1-5T represents a novel species of the genus Nocardioides, for which the name Nocardioides daeguensis sp. nov. is proposed, with the type strain 2C1-5T (=JCM 17460T=KCTC 19799T).",
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Nocardioides daeguensis sp. nov., a nitratereducing bacterium isolated from activated sludge of an industrial wastewater treatment plant. / Cui, Yingshun; Woo, Sung Geun; Lee, Jangho; Sinha, Sahastranshu; Kang, Myung Suk; Jin, Long; Kim, Kwang Kyu; Park, Joonhong; Lee, Myungjin; Lee, Sung Taik.

In: International Journal of Systematic and Evolutionary Microbiology, Vol. 63, No. PART10, 01.10.2013, p. 3727-3732.

Research output: Contribution to journalArticle

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AU - Cui, Yingshun

AU - Woo, Sung Geun

AU - Lee, Jangho

AU - Sinha, Sahastranshu

AU - Kang, Myung Suk

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AU - Lee, Sung Taik

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N2 - A Gram-reaction-positive, rod-shaped, non-spore-forming bacterium (strain 2C1-5T) was isolated from activated sludge of an industrial wastewater treatment plant in Daegu, South Korea. Its taxonomic position was investigated by using a polyphasic approach. On the basis of 16S rRNA gene sequence similarity, the closest phylogenetic relatives were the type strains of Nocardioides nitrophenolicus (98.6% similarity), N. kongjuensis (98.5 %), N. caeni (98.4 %), N. simplex (98.3 %), N. aromaticivorans (98.1 %) and N. ginsengisoli (97.5 %); the phylogenetic distance from other species with validly published names within the genus Nocardioides was greater than 3%. Strain 2C1-5T was characterized chemotaxonomically as having LL-2, 6-diaminopimelic acid in the cell-wall peptidoglycan, MK-8(H4) as the predominant menaquinone and iso-C16: 0, C16: 0 and C17: 1ω6c as the major fatty acids. The G+C content of the genomic DNA was 74.9 mol%. These chemotaxonomic properties and phenotypic characteristics supported the affiliation of strain 2C1-5T to the genus Nocardioides. The results of physiological and biochemical tests allowed genotypic and phenotypic differentiation of strain 2C1-5T from existing species with validly published names. Therefore, strain 2C1-5T represents a novel species of the genus Nocardioides, for which the name Nocardioides daeguensis sp. nov. is proposed, with the type strain 2C1-5T (=JCM 17460T=KCTC 19799T).

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