Nocardioides daeguensis sp. nov., a nitratereducing bacterium isolated from activated sludge of an industrial wastewater treatment plant

Yingshun Cui, Sung Geun Woo, Jangho Lee, Sahastranshu Sinha, Myung Suk Kang, Long Jin, Kwang Kyu Kim, Joonhong Park, Myungjin Lee, Sung Taik Lee

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Abstract

A Gram-reaction-positive, rod-shaped, non-spore-forming bacterium (strain 2C1-5T) was isolated from activated sludge of an industrial wastewater treatment plant in Daegu, South Korea. Its taxonomic position was investigated by using a polyphasic approach. On the basis of 16S rRNA gene sequence similarity, the closest phylogenetic relatives were the type strains of Nocardioides nitrophenolicus (98.6% similarity), N. kongjuensis (98.5 %), N. caeni (98.4 %), N. simplex (98.3 %), N. aromaticivorans (98.1 %) and N. ginsengisoli (97.5 %); the phylogenetic distance from other species with validly published names within the genus Nocardioides was greater than 3%. Strain 2C1-5T was characterized chemotaxonomically as having LL-2, 6-diaminopimelic acid in the cell-wall peptidoglycan, MK-8(H4) as the predominant menaquinone and iso-C16: 0, C16: 0 and C17: 1ω6c as the major fatty acids. The G+C content of the genomic DNA was 74.9 mol%. These chemotaxonomic properties and phenotypic characteristics supported the affiliation of strain 2C1-5T to the genus Nocardioides. The results of physiological and biochemical tests allowed genotypic and phenotypic differentiation of strain 2C1-5T from existing species with validly published names. Therefore, strain 2C1-5T represents a novel species of the genus Nocardioides, for which the name Nocardioides daeguensis sp. nov. is proposed, with the type strain 2C1-5T (=JCM 17460T=KCTC 19799T).

Original languageEnglish
Pages (from-to)3727-3732
Number of pages6
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume63
Issue numberPART10
DOIs
Publication statusPublished - 2013 Oct 1

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All Science Journal Classification (ASJC) codes

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics

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