Non-labeling immunoassay based on zeta-potential analysis

Eun Hang Lee, Yong Hee Lee, Jae Chul Pyun

Research output: Contribution to journalConference article

2 Citations (Scopus)

Abstract

Immunoassay has been widely used for the detection of target analytes in complex mixtures such as blood by using highly specific antigen-antibody interactions. In this work, antibodies against a target analyte was immobilized to magnetic beads and the change of surface charge of magnetic beads were measured after the binding of target proteins by using a zeta potential analyzer. For the feasibility test of the zeta potential analysis, horseradish peroxidase (HRP) was used as a model target analyte. The application to medical diagnosis was demonstrated by detection of C-reactive protein (CRP) which is known to be a biomarker for inflammatory diseases.

Original languageEnglish
Pages (from-to)1023-1024
Number of pages2
JournalProcedia Engineering
Volume47
DOIs
Publication statusPublished - 2012 Jan 1
Event26th European Conference on Solid-State Transducers, EUROSENSOR 2012 - Krakow, Poland
Duration: 2012 Sep 92012 Sep 12

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Zeta potential
Antibodies
Proteins
Biomarkers
Antigens
Surface charge
Blood

All Science Journal Classification (ASJC) codes

  • Engineering(all)

Cite this

Lee, Eun Hang ; Lee, Yong Hee ; Pyun, Jae Chul. / Non-labeling immunoassay based on zeta-potential analysis. In: Procedia Engineering. 2012 ; Vol. 47. pp. 1023-1024.
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Non-labeling immunoassay based on zeta-potential analysis. / Lee, Eun Hang; Lee, Yong Hee; Pyun, Jae Chul.

In: Procedia Engineering, Vol. 47, 01.01.2012, p. 1023-1024.

Research output: Contribution to journalConference article

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