Non-thermal atmospheric pressure plasma functionalized dental implant for enhancement of bacterial resistance and osseointegration

Jung Hwan Lee, Won Seok Jeong, Seog Jin Seo, Hae Won Kim, Kyoung Nam Kim, Eun Ha Choi, Kwangmahn Kim

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Objective Even though roughened titanium (Ti) and Ti alloys have been clinically used as dental implant, they encourage bacterial adhesion, leading to failure of the initial stability. Here, the non-thermal atmospheric pressure plasma jet (NTAPPJ) functionalized Ti and Ti alloy were investigated to promote cellular activities but inhibit the initial attachment of the adherent pioneer bacterium, Streptococcus sanguinis, without topographical changes. Methods After the produced radicals from NTAPPJ were characterized, bacterial adhesion to specimens was assessed by PrestoBlue assay and live-dead staining with or without the NTAPPJ functionalizing. After the surface was characterized using optical profilometry, X-ray photoelectron spectroscopy and contact angle analysis, the ions released from the specimens were investigated. In vitro initial cell attachment (4 h or 24 h) with adhesion images and alkaline phosphatase activity (ALP, 14 days) measurements were performed using rat bone marrow-derived mesenchymal stem cells. Results The initial bacterial adhesion to the Ti and Ti alloy was significantly inhibited after NTAPPJ functionalizing (p < 0.05) compared to those without NTAPPJ functionalizing. The bacterial adhesion-resistance effect was induced by carbon cleaning, which was dependent on the working gas used on the Ti specimens (nitrogen > ammonia and air, p < 0.05). The initial cell adhesion with well-developed vinculin localization and consequent ALP activity at 14 days to the NTAPPJ-functionalized specimens were superior to the non-treated specimens. Significance For the promising success of dental implants, NTAPPJ functionalizing is suggested as a novel surface modification technique; this technique can help ensure the success of integration between the dental implants and bone tissues with less concern of inflammation.

Original languageEnglish
Pages (from-to)257-270
Number of pages14
JournalDental Materials
Volume33
Issue number3
DOIs
Publication statusPublished - 2017 Mar 1

Fingerprint

Plasma Gases
Osseointegration
Dental prostheses
Plasma jets
Dental Implants
Titanium
Atmospheric pressure
Bacterial Adhesion
Plasmas
Adhesion
Titanium alloys
Bone
Vinculin
Photoelectron Spectroscopy
Profilometry
Cell adhesion
Phosphatases
Streptococcus
Stem cells
Mesenchymal Stromal Cells

All Science Journal Classification (ASJC) codes

  • Materials Science(all)
  • Dentistry(all)
  • Mechanics of Materials

Cite this

Lee, Jung Hwan ; Jeong, Won Seok ; Seo, Seog Jin ; Kim, Hae Won ; Kim, Kyoung Nam ; Choi, Eun Ha ; Kim, Kwangmahn. / Non-thermal atmospheric pressure plasma functionalized dental implant for enhancement of bacterial resistance and osseointegration. In: Dental Materials. 2017 ; Vol. 33, No. 3. pp. 257-270.
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abstract = "Objective Even though roughened titanium (Ti) and Ti alloys have been clinically used as dental implant, they encourage bacterial adhesion, leading to failure of the initial stability. Here, the non-thermal atmospheric pressure plasma jet (NTAPPJ) functionalized Ti and Ti alloy were investigated to promote cellular activities but inhibit the initial attachment of the adherent pioneer bacterium, Streptococcus sanguinis, without topographical changes. Methods After the produced radicals from NTAPPJ were characterized, bacterial adhesion to specimens was assessed by PrestoBlue assay and live-dead staining with or without the NTAPPJ functionalizing. After the surface was characterized using optical profilometry, X-ray photoelectron spectroscopy and contact angle analysis, the ions released from the specimens were investigated. In vitro initial cell attachment (4 h or 24 h) with adhesion images and alkaline phosphatase activity (ALP, 14 days) measurements were performed using rat bone marrow-derived mesenchymal stem cells. Results The initial bacterial adhesion to the Ti and Ti alloy was significantly inhibited after NTAPPJ functionalizing (p < 0.05) compared to those without NTAPPJ functionalizing. The bacterial adhesion-resistance effect was induced by carbon cleaning, which was dependent on the working gas used on the Ti specimens (nitrogen > ammonia and air, p < 0.05). The initial cell adhesion with well-developed vinculin localization and consequent ALP activity at 14 days to the NTAPPJ-functionalized specimens were superior to the non-treated specimens. Significance For the promising success of dental implants, NTAPPJ functionalizing is suggested as a novel surface modification technique; this technique can help ensure the success of integration between the dental implants and bone tissues with less concern of inflammation.",
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Non-thermal atmospheric pressure plasma functionalized dental implant for enhancement of bacterial resistance and osseointegration. / Lee, Jung Hwan; Jeong, Won Seok; Seo, Seog Jin; Kim, Hae Won; Kim, Kyoung Nam; Choi, Eun Ha; Kim, Kwangmahn.

In: Dental Materials, Vol. 33, No. 3, 01.03.2017, p. 257-270.

Research output: Contribution to journalArticle

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T1 - Non-thermal atmospheric pressure plasma functionalized dental implant for enhancement of bacterial resistance and osseointegration

AU - Lee, Jung Hwan

AU - Jeong, Won Seok

AU - Seo, Seog Jin

AU - Kim, Hae Won

AU - Kim, Kyoung Nam

AU - Choi, Eun Ha

AU - Kim, Kwangmahn

PY - 2017/3/1

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N2 - Objective Even though roughened titanium (Ti) and Ti alloys have been clinically used as dental implant, they encourage bacterial adhesion, leading to failure of the initial stability. Here, the non-thermal atmospheric pressure plasma jet (NTAPPJ) functionalized Ti and Ti alloy were investigated to promote cellular activities but inhibit the initial attachment of the adherent pioneer bacterium, Streptococcus sanguinis, without topographical changes. Methods After the produced radicals from NTAPPJ were characterized, bacterial adhesion to specimens was assessed by PrestoBlue assay and live-dead staining with or without the NTAPPJ functionalizing. After the surface was characterized using optical profilometry, X-ray photoelectron spectroscopy and contact angle analysis, the ions released from the specimens were investigated. In vitro initial cell attachment (4 h or 24 h) with adhesion images and alkaline phosphatase activity (ALP, 14 days) measurements were performed using rat bone marrow-derived mesenchymal stem cells. Results The initial bacterial adhesion to the Ti and Ti alloy was significantly inhibited after NTAPPJ functionalizing (p < 0.05) compared to those without NTAPPJ functionalizing. The bacterial adhesion-resistance effect was induced by carbon cleaning, which was dependent on the working gas used on the Ti specimens (nitrogen > ammonia and air, p < 0.05). The initial cell adhesion with well-developed vinculin localization and consequent ALP activity at 14 days to the NTAPPJ-functionalized specimens were superior to the non-treated specimens. Significance For the promising success of dental implants, NTAPPJ functionalizing is suggested as a novel surface modification technique; this technique can help ensure the success of integration between the dental implants and bone tissues with less concern of inflammation.

AB - Objective Even though roughened titanium (Ti) and Ti alloys have been clinically used as dental implant, they encourage bacterial adhesion, leading to failure of the initial stability. Here, the non-thermal atmospheric pressure plasma jet (NTAPPJ) functionalized Ti and Ti alloy were investigated to promote cellular activities but inhibit the initial attachment of the adherent pioneer bacterium, Streptococcus sanguinis, without topographical changes. Methods After the produced radicals from NTAPPJ were characterized, bacterial adhesion to specimens was assessed by PrestoBlue assay and live-dead staining with or without the NTAPPJ functionalizing. After the surface was characterized using optical profilometry, X-ray photoelectron spectroscopy and contact angle analysis, the ions released from the specimens were investigated. In vitro initial cell attachment (4 h or 24 h) with adhesion images and alkaline phosphatase activity (ALP, 14 days) measurements were performed using rat bone marrow-derived mesenchymal stem cells. Results The initial bacterial adhesion to the Ti and Ti alloy was significantly inhibited after NTAPPJ functionalizing (p < 0.05) compared to those without NTAPPJ functionalizing. The bacterial adhesion-resistance effect was induced by carbon cleaning, which was dependent on the working gas used on the Ti specimens (nitrogen > ammonia and air, p < 0.05). The initial cell adhesion with well-developed vinculin localization and consequent ALP activity at 14 days to the NTAPPJ-functionalized specimens were superior to the non-treated specimens. Significance For the promising success of dental implants, NTAPPJ functionalizing is suggested as a novel surface modification technique; this technique can help ensure the success of integration between the dental implants and bone tissues with less concern of inflammation.

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