Non-thermal atmospheric pressure plasma induces epigenetic modifications that activate the expression of various cytokines and growth factors in human mesoderm-derived stem cells

Jeongyeon Park, Donghwa Suh, Tianyu Tang, Hae June Lee, Jae Seok Roe, Gyoo Cheon Kim, Sungbum Han, Kiwon Song

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Non-thermal atmospheric pressure plasma (NTAPP) has been reported to induce wound healing, activation of immune cells, and proliferation of mesoderm-derived adult stem cells in human. However, the mechanism by which NTAPP activates these physiological effects is poorly understood. Here, we examined whole genome expression profiles of adipose tissue-derived stem cells (ASCs), the proliferation of which is induced by NTAPP. NTAPP upregulated the expression of genes for cytokine and growth factor, but downregulated genes in apoptotic pathways. When ASCs were treated with NTAPP in the presence of a nitric oxide (NO) scavenger, the expression of various cytokines and growth factors decreased, suggesting that NO is primarily responsible for the enhanced cytokine and growth factor expression induced by NTAPP. Increased histone deacetyl transferase 1 (HDAC1) and decreased acetylated histone 3 were detected in NTAPP-treated ASCs. Similarly, ASCs pre-treated with HDAC, DNA methylation, or histone methylation inhibitors had reduced expression of cytokines and growth factors after NTAPP treatment. Taken together, these results strongly suggest that NTAPP induces epigenetic modifications that activate the expression of cytokines and growth factors, explaining how NTAPP acts as an efficient tool in regenerative medicine to stimulate stem cell proliferation, to activate immune cells, and to recover wounds.

Original languageEnglish
Pages (from-to)108-122
Number of pages15
JournalFree Radical Biology and Medicine
Volume148
DOIs
Publication statusPublished - 2020 Feb 20

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Physiology (medical)

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