Non-thermal atmospheric pressure plasma preferentially induces apoptosis in p53-mutated cancer cells by activating ROS stress-response pathways

Yonghao Ma, Chang Seung Ha, Seok Won Hwang, Hae June Lee, Gyoo Cheon Kim, Kyo Won Lee, Kiwon Song

Research output: Contribution to journalArticle

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Abstract

Non-thermal atmospheric pressure plasma (NTAPP) is an ionized gas at room temperature and has potential as a new apoptosis-promoting cancer therapy that acts by generating reactive oxygen species (ROS). However, it is imperative to determine its selectivity and standardize the components and composition of NTAPP. Here, we designed an NTAPP-generating apparatus combined with a He gas feeding system and demonstrated its high selectivity toward p53-mutated cancer cells. We first determined the proper conditions for NTAPP exposure to selectively induce apoptosis in cancer cells. The apoptotic effect of NTAPP was greater for p53-mutated cancer cells; artificial p53 expression in p53-negative HT29 cells decreased the pro-apoptotic effect of NTAPP. We also examined extra- and intracellular ROS levels in NTAPP-treated cells to deduce the mechanism of NTAPP action. While NTAPP-mediated increases in extracellular nitric oxide (NO) did not affect cell viability, intracellular ROS increased under NTAPP exposure and induced apoptotic cell death. This effect was dose-dependently reduced following treatment with ROS scavengers. NTAPP induced apoptosis even in doxorubicin-resistant cancer cell lines, demonstrating the feasibility of NTAPP as a potent cancer therapy. Collectively, these results strongly support the potential of NTAPP as a selective anticancer treatment, especially for p53-mutated cancer cells.

Original languageEnglish
Article numbere91947
JournalPLoS One
Volume9
Issue number4
DOIs
Publication statusPublished - 2014 Apr 23

Fingerprint

Plasma Gases
atmospheric pressure
reactive oxygen species
Reactive Oxygen Species
stress response
apoptosis
Cells
Apoptosis
Neoplasms
neoplasm cells
Gases
gases
HT29 Cells
therapeutics
neoplasms
doxorubicin
Cell death
Plasma Cells
Doxorubicin

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Ma, Yonghao ; Ha, Chang Seung ; Hwang, Seok Won ; Lee, Hae June ; Kim, Gyoo Cheon ; Lee, Kyo Won ; Song, Kiwon. / Non-thermal atmospheric pressure plasma preferentially induces apoptosis in p53-mutated cancer cells by activating ROS stress-response pathways. In: PLoS One. 2014 ; Vol. 9, No. 4.
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Non-thermal atmospheric pressure plasma preferentially induces apoptosis in p53-mutated cancer cells by activating ROS stress-response pathways. / Ma, Yonghao; Ha, Chang Seung; Hwang, Seok Won; Lee, Hae June; Kim, Gyoo Cheon; Lee, Kyo Won; Song, Kiwon.

In: PLoS One, Vol. 9, No. 4, e91947, 23.04.2014.

Research output: Contribution to journalArticle

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