Nonhomologous end-joining ligation transfers DNA regulatory elements between cointroduced plasmids

Toshio Ishikawa, Eunjig Lee, J. Larry Jameson

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Cointroduction of plasmids into mammalian cells is commonly used to investigate transcription factor regulation of reporter genes or to normalize transfection efficiency. We report here that cotransfected DNA molecules commonly transfer enhancer elements from one plasmid to another. Using separate Renilla or Firefly luciferase reporters, we found that an estrogen response element (ERE) originally linked to one of the reporters stimulated expression of the non-ERE-containing reporter. Similar enhancer transfer was seen with the cytomegalovirus enhancer. This enhancer transfer effect was not seen when cells were transfected separately with the reporters and the extracts were then combined before luciferase assays. The degree of enhancer transfer increased with transfected plasmid concentration and was greater when linearized rather than circular plasmid DNA was used. We hypothesized that double-strand breaks and heteroligation of cointroduced DNA molecules mediated the transfer of regulatory elements from one molecule to another. PCR of transfected plasmid DNA confirmed nonhomologous end-joining (NHEJ) ligation of DNA fragments originally present in separate plasmids. The NHEJ reaction was enhanced by UV light treatment to introduce double-strand breaks, and it was greater after liposome-mediated transfection than after calcium-phosphate-mediated transfection. NHEJ also occurred after adenoviral transfer of DNA into cells. We conclude that NHEJ mediates the transfer of regulatory DNA elements among cointroduced DNA molecules. These findings indicate the need for caution when interpreting results of transfection experiments containing more than one plasmid and suggest a mechanism whereby viruses or other exogenous DNA might recombine to activate unrelated genes.

Original languageEnglish
Pages (from-to)8323-8331
Number of pages9
JournalMolecular and Cellular Biology
Volume24
Issue number19
DOIs
Publication statusPublished - 2004 Oct 1

Fingerprint

Ligation
Plasmids
DNA
Transfection
Response Elements
DNA End-Joining Repair
Renilla Luciferases
Firefly Luciferases
Circular DNA
Ultraviolet Rays
Luciferases
Cytomegalovirus
Reporter Genes
Liposomes
Estrogens
Transcription Factors
Viruses
Polymerase Chain Reaction
Genes

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cell Biology

Cite this

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Nonhomologous end-joining ligation transfers DNA regulatory elements between cointroduced plasmids. / Ishikawa, Toshio; Lee, Eunjig; Jameson, J. Larry.

In: Molecular and Cellular Biology, Vol. 24, No. 19, 01.10.2004, p. 8323-8331.

Research output: Contribution to journalArticle

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