Noninvasive photodetachment of stem cells on tunable conductive polymer nano thin films: Selective harvesting and preserved differentiation capacity

Jungmok You, June Seok Heo, Jeonghun Kim, Teahoon Park, Byeonggwan Kim, Han Soo Kim, Youjeong Choi, Hyun Ok Kim, Eunkyoung Kim

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Viable mesenchymal stem cells (MSCs) were efficiently and selectively harvested by near-infrared (NIR) light using the photothermal effect of a conductive polymer nano thin film. The poly(3,4-ethylenedioxy thiophene) (PEDOT)-coated cell culture surfaces were prepared via a simple and fast solution-casting polymerization (SCP) technique. The absorption of PEDOT thin films in the NIR region was effectively triggered cell harvesting upon exposure to an NIR source. By controlling the NIR absorption of the PEDOT film through electrochemical doping or growing PEDOT with different thin film thickness from 70 to 300 nm, the proliferation and harvesting of MSCs on the PEDOT surface were controlled quantitatively. This light-induced cell detachment method based on PEDOT films provides the temporal and spatial control of cell harvesting, as well as cell patterning. The harvested stem cells were found to be alive and well proliferated despite the use of temperature increase by NIR. More importantly, the harvested MSCs by this method preserved their intrinsic characteristics as well as multilineage differentiation capacities. This PEDOT surfaces could be used for repetitive culture and detachment of MSCs or for efficient selection or depletion of a specific subset from heterogeneous population during culture of various tissue-derived cells because there were no photodegradation and photobreakage in the PEDOT films by NIR exposure.

Original languageEnglish
Pages (from-to)4119-4128
Number of pages10
JournalACS Nano
Volume7
Issue number5
DOIs
Publication statusPublished - 2013 May 28

Fingerprint

Thiophenes
photodetachment
stem cells
Thiophene
thiophenes
Stem cells
Polymers
Thin films
polymers
thin films
Infrared radiation
cells
Cell culture
detachment
Photodegradation
Infrared absorption
infrared absorption
set theory
Film thickness
Light sources

All Science Journal Classification (ASJC) codes

  • Materials Science(all)
  • Engineering(all)
  • Physics and Astronomy(all)

Cite this

You, Jungmok ; Heo, June Seok ; Kim, Jeonghun ; Park, Teahoon ; Kim, Byeonggwan ; Kim, Han Soo ; Choi, Youjeong ; Kim, Hyun Ok ; Kim, Eunkyoung. / Noninvasive photodetachment of stem cells on tunable conductive polymer nano thin films : Selective harvesting and preserved differentiation capacity. In: ACS Nano. 2013 ; Vol. 7, No. 5. pp. 4119-4128.
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Noninvasive photodetachment of stem cells on tunable conductive polymer nano thin films : Selective harvesting and preserved differentiation capacity. / You, Jungmok; Heo, June Seok; Kim, Jeonghun; Park, Teahoon; Kim, Byeonggwan; Kim, Han Soo; Choi, Youjeong; Kim, Hyun Ok; Kim, Eunkyoung.

In: ACS Nano, Vol. 7, No. 5, 28.05.2013, p. 4119-4128.

Research output: Contribution to journalArticle

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