Novel acquired metallo-β-lactamase gene, blaSIM-1, in a class 1 integron from Acinetobacter baumannii clinical isolates from Korea

Kyungwon Lee, Jong Hwa Yum, DongEun Yong, Hyuk Min Lee, HeungDong Kim, Jean Denis Docquier, Gian Maria Rossolini, Yunsop Chong

Research output: Contribution to journalArticle

260 Citations (Scopus)

Abstract

Carbapenem resistance mediated by acquired carbapenemase genes has been increasingly reported, particularly for clinical isolates of Pseudomonas aeruginosa and Acinetobacter spp. Of 1,234 nonduplicate isolates of carbapenem-resistant Pseudomonas spp. and Acinetobacter spp. isolated at a tertiary-care hospital in Seoul, Korea, 211 (17%) were positive for metallo-β-lactamase (MBL). Of these, 204 (96%) had either the bla IMP-1 or blaVIM-2 allele. In addition, seven Acinetobacter baumannii isolates were found to have a novel MBL gene, which was designated blaSIM-1. The SIM-1 protein has a pI of 7.2, is a new member of subclass B1, and exhibits 64 to 69% identity with the IMF-type MBLs, which are its closest relatives. All SIM-1-producing isolates exhibited relatively low imipenem and meropenem MICs (8 to 16 μg/ml) and had a multidrug resistance phenotype. Expression of the cloned blaSIM-1 gene in Escherichia coli revealed that the encoded enzyme is capable of hydrolyzing a broad array of β-lactams, including penicillins, narrow- to expanded-spectrum cephalosporins, and carbapenems. The blaSIM-1 gene was carried on a gene cassette inserted into a class 1 integron, which included three additional cassettes (arr-3, catB3, and aadA1). The strains were isolated from sputum and urine specimens from patients with pneumonia and urinary tract infections, respectively. All patients had various underlying diseases. Pulsed-field gel electrophoresis of SmaI-digested genomic DNAs showed that the strains belonged to two different clonal lineages, indicating that horizontal transfer of this gene had occurred and suggesting the possibility of further spread of resistance in the future.

Original languageEnglish
Pages (from-to)4485-4491
Number of pages7
JournalAntimicrobial Agents and Chemotherapy
Volume49
Issue number11
DOIs
Publication statusPublished - 2005 Nov 1

Fingerprint

Integrons
Acinetobacter baumannii
Korea
Carbapenems
Acinetobacter
Genes
meropenem
Lactams
Horizontal Gene Transfer
Inosine Monophosphate
Imipenem
Pulsed Field Gel Electrophoresis
Multiple Drug Resistance
Tertiary Healthcare
Cephalosporins
Pseudomonas
Sputum
Tertiary Care Centers
Urinary Tract Infections
Penicillins

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Pharmacology (medical)
  • Infectious Diseases

Cite this

Lee, Kyungwon ; Yum, Jong Hwa ; Yong, DongEun ; Lee, Hyuk Min ; Kim, HeungDong ; Docquier, Jean Denis ; Rossolini, Gian Maria ; Chong, Yunsop. / Novel acquired metallo-β-lactamase gene, blaSIM-1, in a class 1 integron from Acinetobacter baumannii clinical isolates from Korea. In: Antimicrobial Agents and Chemotherapy. 2005 ; Vol. 49, No. 11. pp. 4485-4491.
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abstract = "Carbapenem resistance mediated by acquired carbapenemase genes has been increasingly reported, particularly for clinical isolates of Pseudomonas aeruginosa and Acinetobacter spp. Of 1,234 nonduplicate isolates of carbapenem-resistant Pseudomonas spp. and Acinetobacter spp. isolated at a tertiary-care hospital in Seoul, Korea, 211 (17{\%}) were positive for metallo-β-lactamase (MBL). Of these, 204 (96{\%}) had either the bla IMP-1 or blaVIM-2 allele. In addition, seven Acinetobacter baumannii isolates were found to have a novel MBL gene, which was designated blaSIM-1. The SIM-1 protein has a pI of 7.2, is a new member of subclass B1, and exhibits 64 to 69{\%} identity with the IMF-type MBLs, which are its closest relatives. All SIM-1-producing isolates exhibited relatively low imipenem and meropenem MICs (8 to 16 μg/ml) and had a multidrug resistance phenotype. Expression of the cloned blaSIM-1 gene in Escherichia coli revealed that the encoded enzyme is capable of hydrolyzing a broad array of β-lactams, including penicillins, narrow- to expanded-spectrum cephalosporins, and carbapenems. The blaSIM-1 gene was carried on a gene cassette inserted into a class 1 integron, which included three additional cassettes (arr-3, catB3, and aadA1). The strains were isolated from sputum and urine specimens from patients with pneumonia and urinary tract infections, respectively. All patients had various underlying diseases. Pulsed-field gel electrophoresis of SmaI-digested genomic DNAs showed that the strains belonged to two different clonal lineages, indicating that horizontal transfer of this gene had occurred and suggesting the possibility of further spread of resistance in the future.",
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Novel acquired metallo-β-lactamase gene, blaSIM-1, in a class 1 integron from Acinetobacter baumannii clinical isolates from Korea. / Lee, Kyungwon; Yum, Jong Hwa; Yong, DongEun; Lee, Hyuk Min; Kim, HeungDong; Docquier, Jean Denis; Rossolini, Gian Maria; Chong, Yunsop.

In: Antimicrobial Agents and Chemotherapy, Vol. 49, No. 11, 01.11.2005, p. 4485-4491.

Research output: Contribution to journalArticle

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T1 - Novel acquired metallo-β-lactamase gene, blaSIM-1, in a class 1 integron from Acinetobacter baumannii clinical isolates from Korea

AU - Lee, Kyungwon

AU - Yum, Jong Hwa

AU - Yong, DongEun

AU - Lee, Hyuk Min

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AU - Docquier, Jean Denis

AU - Rossolini, Gian Maria

AU - Chong, Yunsop

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