Novel ano1 inhibitor from mallotus apelta extract exerts anticancer activity through downregulation of ano1

Yohan Seo; Nguyen Hoang Anh; Yunkyung Heo; So Hyeon Park; Phan Van Kiem; Yechan Lee; Duong Thi Hai Yen; Sungwoo Jo; Dongkyu Jeon; Bui Huu Tai; Nguyen Hoai Nam; Chau Van Minh; Seung Hyun Kim; Nguyen Xuan Nhiem; Wan Namkung

doi:10.3390/ijms21186470

Novel ano1 inhibitor from mallotus apelta extract exerts anticancer activity through downregulation of ano1

Yohan Seo, Nguyen Hoang Anh, Yunkyung Heo, So Hyeon Park, Phan Van Kiem, Yechan Lee, Duong Thi Hai Yen, Sungwoo Jo, Dongkyu Jeon, Bui Huu Tai, Nguyen Hoai Nam, Chau Van Minh, Seung Hyun Kim, Nguyen Xuan Nhiem, Wan Namkung

Department of Pharmacy

Research output: Contribution to journal › Article › peer-review

8 Citations (Scopus)

Abstract

Anoctamin1 (ANO1), a calcium-activated chloride channel, is frequently overexpressed in several cancers, including human prostate cancer and oral squamous cell carcinomas. ANO1 plays a critical role in tumor growth and maintenance of these cancers. In this study, we have isolated two new compounds (1 and 2) and four known compounds (3–6) from Mallotus apelta. These compounds were evaluated for their inhibitory effects on ANO1 channel activity and their cytotoxic effects on PC-3 prostate cancer cells. Interestingly, compounds 1 and 2 significantly reduced both ANO1 channel activity and cell viability. Electrophysiological study revealed that compound 2 (Ani-D2) is a potent and selective ANO1 inhibitor, with an IC₅₀ value of 2.64 µM. Ani-D2 had minimal effect on cystic fibrosis transmembrane conductance regulator (CFTR) chloride channel activity and intracellular calcium signaling. Notably, Ani-D2 significantly reduced ANO1 protein expression levels and cell viability in an ANO1-dependent manner in PC-3 and oral squamous cell carcinoma CAL-27 cells. In addition, Ani-D2 strongly reduced cell migration and induced activation of caspase-3 and cleavage of PARP in PC-3 and CAL-27 cells. This study revealed that a novel ANO1 inhibitor, Ani-D2, has therapeutic potential for the treatment of several cancers that overexpress ANO1, such as prostate cancer and oral squamous cell carcinoma.

Original language	English
Article number	6470
Pages (from-to)	1-15
Number of pages	15
Journal	International journal of molecular sciences
Volume	21
Issue number	18
DOIs	https://doi.org/10.3390/ijms21186470
Publication status	Published - 2020 Sept 2

Bibliographical note

Funding Information:
Funding: This research was funded by the Vietnam Academy of Science and Technology (DL0000.01/19-20), National Research Foundation of Korea (NRF-2015R1D1A1A01057695, NRF2018R1A6A1A03023718, and NRF-2019R1I1A1A01061117). This research was also supported (in part) by Yonsei University Research Fund 2019 (Project No. 2019-12-0013).

Funding Information:
This research was funded by the Vietnam Academy of Science and Technology (DL0000.01/19-20), National Research Foundation of Korea (NRF-2015R1D1A1A01057695, NRF2018R1A6A1A03023718, and NRF-2019R1I1A1A01061117). This research was also supported (in part) by Yonsei University Research Fund 2019 (Project No. 2019-12-0013). The authors would like to thank Hyejin Jeon (College of Pharmacy, Yonsei University), Pham Hai Yen (Institute of Marine Biochemistry, Vietnam Academy of Science and Technology), and Pham The Chinh (Faculty of Chemistry, Thai Nguyen University of Science) for their support to this study.

Publisher Copyright:
© 2020 by the authors. Licensee MDPI, Basel, Switzerland.

All Science Journal Classification (ASJC) codes

Catalysis
Molecular Biology
Spectroscopy
Computer Science Applications
Physical and Theoretical Chemistry
Organic Chemistry
Inorganic Chemistry

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10.3390/ijms21186470

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Seo, Y., Anh, N. H., Heo, Y., Park, S. H., Kiem, P. V., Lee, Y., Yen, D. T. H., Jo, S., Jeon, D., Tai, B. H., Nam, N. H., Minh, C. V., Kim, S. H., Nhiem, N. X., & Namkung, W. (2020). Novel ano1 inhibitor from mallotus apelta extract exerts anticancer activity through downregulation of ano1. International journal of molecular sciences, 21(18), 1-15. [6470]. https://doi.org/10.3390/ijms21186470

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title = "Novel ano1 inhibitor from mallotus apelta extract exerts anticancer activity through downregulation of ano1",

abstract = "Anoctamin1 (ANO1), a calcium-activated chloride channel, is frequently overexpressed in several cancers, including human prostate cancer and oral squamous cell carcinomas. ANO1 plays a critical role in tumor growth and maintenance of these cancers. In this study, we have isolated two new compounds (1 and 2) and four known compounds (3–6) from Mallotus apelta. These compounds were evaluated for their inhibitory effects on ANO1 channel activity and their cytotoxic effects on PC-3 prostate cancer cells. Interestingly, compounds 1 and 2 significantly reduced both ANO1 channel activity and cell viability. Electrophysiological study revealed that compound 2 (Ani-D2) is a potent and selective ANO1 inhibitor, with an IC50 value of 2.64 µM. Ani-D2 had minimal effect on cystic fibrosis transmembrane conductance regulator (CFTR) chloride channel activity and intracellular calcium signaling. Notably, Ani-D2 significantly reduced ANO1 protein expression levels and cell viability in an ANO1-dependent manner in PC-3 and oral squamous cell carcinoma CAL-27 cells. In addition, Ani-D2 strongly reduced cell migration and induced activation of caspase-3 and cleavage of PARP in PC-3 and CAL-27 cells. This study revealed that a novel ANO1 inhibitor, Ani-D2, has therapeutic potential for the treatment of several cancers that overexpress ANO1, such as prostate cancer and oral squamous cell carcinoma.",

author = "Yohan Seo and Anh, {Nguyen Hoang} and Yunkyung Heo and Park, {So Hyeon} and Kiem, {Phan Van} and Yechan Lee and Yen, {Duong Thi Hai} and Sungwoo Jo and Dongkyu Jeon and Tai, {Bui Huu} and Nam, {Nguyen Hoai} and Minh, {Chau Van} and Kim, {Seung Hyun} and Nhiem, {Nguyen Xuan} and Wan Namkung",

note = "Funding Information: Funding: This research was funded by the Vietnam Academy of Science and Technology (DL0000.01/19-20), National Research Foundation of Korea (NRF-2015R1D1A1A01057695, NRF2018R1A6A1A03023718, and NRF-2019R1I1A1A01061117). This research was also supported (in part) by Yonsei University Research Fund 2019 (Project No. 2019-12-0013). Funding Information: This research was funded by the Vietnam Academy of Science and Technology (DL0000.01/19-20), National Research Foundation of Korea (NRF-2015R1D1A1A01057695, NRF2018R1A6A1A03023718, and NRF-2019R1I1A1A01061117). This research was also supported (in part) by Yonsei University Research Fund 2019 (Project No. 2019-12-0013). The authors would like to thank Hyejin Jeon (College of Pharmacy, Yonsei University), Pham Hai Yen (Institute of Marine Biochemistry, Vietnam Academy of Science and Technology), and Pham The Chinh (Faculty of Chemistry, Thai Nguyen University of Science) for their support to this study. Publisher Copyright: {\textcopyright} 2020 by the authors. Licensee MDPI, Basel, Switzerland.",

year = "2020",

month = sep,

day = "2",

doi = "10.3390/ijms21186470",

language = "English",

volume = "21",

pages = "1--15",

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Seo, Y, Anh, NH, Heo, Y, Park, SH, Kiem, PV, Lee, Y, Yen, DTH, Jo, S, Jeon, D, Tai, BH, Nam, NH, Minh, CV, Kim, SH, Nhiem, NX & Namkung, W 2020, 'Novel ano1 inhibitor from mallotus apelta extract exerts anticancer activity through downregulation of ano1', International journal of molecular sciences, vol. 21, no. 18, 6470, pp. 1-15. https://doi.org/10.3390/ijms21186470

TY - JOUR

T1 - Novel ano1 inhibitor from mallotus apelta extract exerts anticancer activity through downregulation of ano1

AU - Seo, Yohan

AU - Anh, Nguyen Hoang

AU - Heo, Yunkyung

AU - Park, So Hyeon

AU - Kiem, Phan Van

AU - Lee, Yechan

AU - Yen, Duong Thi Hai

AU - Jo, Sungwoo

AU - Jeon, Dongkyu

AU - Tai, Bui Huu

AU - Nam, Nguyen Hoai

AU - Minh, Chau Van

AU - Kim, Seung Hyun

AU - Nhiem, Nguyen Xuan

AU - Namkung, Wan

N1 - Funding Information: Funding: This research was funded by the Vietnam Academy of Science and Technology (DL0000.01/19-20), National Research Foundation of Korea (NRF-2015R1D1A1A01057695, NRF2018R1A6A1A03023718, and NRF-2019R1I1A1A01061117). This research was also supported (in part) by Yonsei University Research Fund 2019 (Project No. 2019-12-0013). Funding Information: This research was funded by the Vietnam Academy of Science and Technology (DL0000.01/19-20), National Research Foundation of Korea (NRF-2015R1D1A1A01057695, NRF2018R1A6A1A03023718, and NRF-2019R1I1A1A01061117). This research was also supported (in part) by Yonsei University Research Fund 2019 (Project No. 2019-12-0013). The authors would like to thank Hyejin Jeon (College of Pharmacy, Yonsei University), Pham Hai Yen (Institute of Marine Biochemistry, Vietnam Academy of Science and Technology), and Pham The Chinh (Faculty of Chemistry, Thai Nguyen University of Science) for their support to this study. Publisher Copyright: © 2020 by the authors. Licensee MDPI, Basel, Switzerland.

PY - 2020/9/2

Y1 - 2020/9/2

N2 - Anoctamin1 (ANO1), a calcium-activated chloride channel, is frequently overexpressed in several cancers, including human prostate cancer and oral squamous cell carcinomas. ANO1 plays a critical role in tumor growth and maintenance of these cancers. In this study, we have isolated two new compounds (1 and 2) and four known compounds (3–6) from Mallotus apelta. These compounds were evaluated for their inhibitory effects on ANO1 channel activity and their cytotoxic effects on PC-3 prostate cancer cells. Interestingly, compounds 1 and 2 significantly reduced both ANO1 channel activity and cell viability. Electrophysiological study revealed that compound 2 (Ani-D2) is a potent and selective ANO1 inhibitor, with an IC50 value of 2.64 µM. Ani-D2 had minimal effect on cystic fibrosis transmembrane conductance regulator (CFTR) chloride channel activity and intracellular calcium signaling. Notably, Ani-D2 significantly reduced ANO1 protein expression levels and cell viability in an ANO1-dependent manner in PC-3 and oral squamous cell carcinoma CAL-27 cells. In addition, Ani-D2 strongly reduced cell migration and induced activation of caspase-3 and cleavage of PARP in PC-3 and CAL-27 cells. This study revealed that a novel ANO1 inhibitor, Ani-D2, has therapeutic potential for the treatment of several cancers that overexpress ANO1, such as prostate cancer and oral squamous cell carcinoma.

AB - Anoctamin1 (ANO1), a calcium-activated chloride channel, is frequently overexpressed in several cancers, including human prostate cancer and oral squamous cell carcinomas. ANO1 plays a critical role in tumor growth and maintenance of these cancers. In this study, we have isolated two new compounds (1 and 2) and four known compounds (3–6) from Mallotus apelta. These compounds were evaluated for their inhibitory effects on ANO1 channel activity and their cytotoxic effects on PC-3 prostate cancer cells. Interestingly, compounds 1 and 2 significantly reduced both ANO1 channel activity and cell viability. Electrophysiological study revealed that compound 2 (Ani-D2) is a potent and selective ANO1 inhibitor, with an IC50 value of 2.64 µM. Ani-D2 had minimal effect on cystic fibrosis transmembrane conductance regulator (CFTR) chloride channel activity and intracellular calcium signaling. Notably, Ani-D2 significantly reduced ANO1 protein expression levels and cell viability in an ANO1-dependent manner in PC-3 and oral squamous cell carcinoma CAL-27 cells. In addition, Ani-D2 strongly reduced cell migration and induced activation of caspase-3 and cleavage of PARP in PC-3 and CAL-27 cells. This study revealed that a novel ANO1 inhibitor, Ani-D2, has therapeutic potential for the treatment of several cancers that overexpress ANO1, such as prostate cancer and oral squamous cell carcinoma.

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JO - International Journal of Molecular Sciences

JF - International Journal of Molecular Sciences

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ER -

Novel ano1 inhibitor from mallotus apelta extract exerts anticancer activity through downregulation of ano1

Abstract

Bibliographical note

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UN SDGs

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