Novel anti-biofouling bioactive calcium silicate-based cement containing 2-methacryloyloxyethyl phosphorylcholine

Jae Sung Kwon, Myung Jin Lee, Ji Young Kim, Dohyun Kim, Jeong Hyun Ryu, Sungil Jang, Kwangmahn Kim, ChungJu Hwang, Sung Hwan Choi

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Calcium silicate-based cements (CSCs) are commonly used for endodontic procedures; however, their antibacterial effects are limited. The objective of this study was to develop a 2-methacryloyloxyethyl phosphorylcholine (MPC)-incorporated CSC with improved antibacterial properties, while maintaining the original advantageous features of CSC. MPC was incorporated into a commercial CSC (Endocem MTA) at 0 wt% (control), 1.5%, 3.0 wt%, 5.0 wt%, 7.5 wt%, and 10 wt%. The setting time, compressive strength, water sorption, and glycerol contact angle were measured. Protein absorption was measured and bacterial adhesion on the surface was evaluated using Enterococcus faecalis. The bactericidal effect was examined by the disc diffusion test. Mineralization ability was assessed based on calcium ion deposition, as assessed by alizarin red staining, after immersion into Hank’s balanced salt solution for 7 days. High concentrations of MPC in CSC (7.5 wt% and 10 wt%) increased the setting time, reduced compressive strength, and reduced wettability. MPC (3 wt%) had greater protein repellent and anti-biofouling effects than those of control and test materials (P < 0.001). However, no bactericidal effect was observed for any control or test materials. There was greater calcium ion deposition on the surface of MPC-supplemented CSC than on the control (P < 0.001). The addition of 3 wt% MPC polymer to CSC confers protein-repellent properties and reduced bacterial attachment, with the potential for improved mineralization.

Original languageEnglish
Article numbere0211007
JournalPloS one
Volume14
Issue number1
DOIs
Publication statusPublished - 2019 Jan 1

Fingerprint

Silicate Cement
Biofouling
calcium silicate
biofouling
cement
Cements
antibacterial properties
Compressive Strength
repellents
strength (mechanics)
Compressive strength
Pemetrexed
mineralization
Ions
ions
Bacterial Adhesion
Calcium
Wettability
calcium
bacterial adhesion

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Kwon, J. S., Lee, M. J., Kim, J. Y., Kim, D., Ryu, J. H., Jang, S., ... Choi, S. H. (2019). Novel anti-biofouling bioactive calcium silicate-based cement containing 2-methacryloyloxyethyl phosphorylcholine. PloS one, 14(1), [e0211007]. https://doi.org/10.1371/journal.pone.0211007
Kwon, Jae Sung ; Lee, Myung Jin ; Kim, Ji Young ; Kim, Dohyun ; Ryu, Jeong Hyun ; Jang, Sungil ; Kim, Kwangmahn ; Hwang, ChungJu ; Choi, Sung Hwan. / Novel anti-biofouling bioactive calcium silicate-based cement containing 2-methacryloyloxyethyl phosphorylcholine. In: PloS one. 2019 ; Vol. 14, No. 1.
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abstract = "Calcium silicate-based cements (CSCs) are commonly used for endodontic procedures; however, their antibacterial effects are limited. The objective of this study was to develop a 2-methacryloyloxyethyl phosphorylcholine (MPC)-incorporated CSC with improved antibacterial properties, while maintaining the original advantageous features of CSC. MPC was incorporated into a commercial CSC (Endocem MTA) at 0 wt{\%} (control), 1.5{\%}, 3.0 wt{\%}, 5.0 wt{\%}, 7.5 wt{\%}, and 10 wt{\%}. The setting time, compressive strength, water sorption, and glycerol contact angle were measured. Protein absorption was measured and bacterial adhesion on the surface was evaluated using Enterococcus faecalis. The bactericidal effect was examined by the disc diffusion test. Mineralization ability was assessed based on calcium ion deposition, as assessed by alizarin red staining, after immersion into Hank’s balanced salt solution for 7 days. High concentrations of MPC in CSC (7.5 wt{\%} and 10 wt{\%}) increased the setting time, reduced compressive strength, and reduced wettability. MPC (3 wt{\%}) had greater protein repellent and anti-biofouling effects than those of control and test materials (P < 0.001). However, no bactericidal effect was observed for any control or test materials. There was greater calcium ion deposition on the surface of MPC-supplemented CSC than on the control (P < 0.001). The addition of 3 wt{\%} MPC polymer to CSC confers protein-repellent properties and reduced bacterial attachment, with the potential for improved mineralization.",
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Novel anti-biofouling bioactive calcium silicate-based cement containing 2-methacryloyloxyethyl phosphorylcholine. / Kwon, Jae Sung; Lee, Myung Jin; Kim, Ji Young; Kim, Dohyun; Ryu, Jeong Hyun; Jang, Sungil; Kim, Kwangmahn; Hwang, ChungJu; Choi, Sung Hwan.

In: PloS one, Vol. 14, No. 1, e0211007, 01.01.2019.

Research output: Contribution to journalArticle

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AU - Kwon, Jae Sung

AU - Lee, Myung Jin

AU - Kim, Ji Young

AU - Kim, Dohyun

AU - Ryu, Jeong Hyun

AU - Jang, Sungil

AU - Kim, Kwangmahn

AU - Hwang, ChungJu

AU - Choi, Sung Hwan

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N2 - Calcium silicate-based cements (CSCs) are commonly used for endodontic procedures; however, their antibacterial effects are limited. The objective of this study was to develop a 2-methacryloyloxyethyl phosphorylcholine (MPC)-incorporated CSC with improved antibacterial properties, while maintaining the original advantageous features of CSC. MPC was incorporated into a commercial CSC (Endocem MTA) at 0 wt% (control), 1.5%, 3.0 wt%, 5.0 wt%, 7.5 wt%, and 10 wt%. The setting time, compressive strength, water sorption, and glycerol contact angle were measured. Protein absorption was measured and bacterial adhesion on the surface was evaluated using Enterococcus faecalis. The bactericidal effect was examined by the disc diffusion test. Mineralization ability was assessed based on calcium ion deposition, as assessed by alizarin red staining, after immersion into Hank’s balanced salt solution for 7 days. High concentrations of MPC in CSC (7.5 wt% and 10 wt%) increased the setting time, reduced compressive strength, and reduced wettability. MPC (3 wt%) had greater protein repellent and anti-biofouling effects than those of control and test materials (P < 0.001). However, no bactericidal effect was observed for any control or test materials. There was greater calcium ion deposition on the surface of MPC-supplemented CSC than on the control (P < 0.001). The addition of 3 wt% MPC polymer to CSC confers protein-repellent properties and reduced bacterial attachment, with the potential for improved mineralization.

AB - Calcium silicate-based cements (CSCs) are commonly used for endodontic procedures; however, their antibacterial effects are limited. The objective of this study was to develop a 2-methacryloyloxyethyl phosphorylcholine (MPC)-incorporated CSC with improved antibacterial properties, while maintaining the original advantageous features of CSC. MPC was incorporated into a commercial CSC (Endocem MTA) at 0 wt% (control), 1.5%, 3.0 wt%, 5.0 wt%, 7.5 wt%, and 10 wt%. The setting time, compressive strength, water sorption, and glycerol contact angle were measured. Protein absorption was measured and bacterial adhesion on the surface was evaluated using Enterococcus faecalis. The bactericidal effect was examined by the disc diffusion test. Mineralization ability was assessed based on calcium ion deposition, as assessed by alizarin red staining, after immersion into Hank’s balanced salt solution for 7 days. High concentrations of MPC in CSC (7.5 wt% and 10 wt%) increased the setting time, reduced compressive strength, and reduced wettability. MPC (3 wt%) had greater protein repellent and anti-biofouling effects than those of control and test materials (P < 0.001). However, no bactericidal effect was observed for any control or test materials. There was greater calcium ion deposition on the surface of MPC-supplemented CSC than on the control (P < 0.001). The addition of 3 wt% MPC polymer to CSC confers protein-repellent properties and reduced bacterial attachment, with the potential for improved mineralization.

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