Gene-targeted FLX titanium pyrosequencing integrated with stable isotope probing (SIP) using [13C]biphenyl substrate revealed that tidal mudflat sediments harbor novel aromatic ring hydroxylating dioxygenases (ARHD). More than 80% of the detected ARHD genes comprise four clades (0.5 distance) with 49 to 70% amino acid identity to sequences in public databases. The 16S rRNA sequences enriched in the 13C fraction were from the Betaproteobacteria, bacilli (primarily Paenibacillus-like), and unclassified phyla.
All Science Journal Classification (ASJC) codes
- Food Science
- Applied Microbiology and Biotechnology