Novel cyanine dyes with vinylsulfone group for labeling biomolecules

Jin Woo Park, YoungSoo Kim, Kee Jung Lee, Dong Jin Kim

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Novel vinylsulfone cyanine dyes (em. 550-850 nm) were designed and synthesized for fluorescent labeling of biomolecules via 1,2-addition reaction in aqueous conditions. Due to the virtue of chemical structures of both fluorophore and reactive group, these dyes could be significantly stable and reactive in various aqueous/organic conditions. A wide variety of pH, temperature, buffer concentration, and protein were tested for the optimal labeling condition.

Original languageEnglish
Pages (from-to)350-362
Number of pages13
JournalBioconjugate Chemistry
Volume23
Issue number3
DOIs
Publication statusPublished - 2012 Mar 21

Fingerprint

Biomolecules
Labeling
Coloring Agents
Dyes
Addition reactions
Fluorophores
Buffers
Proteins
Temperature

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering
  • Pharmacology
  • Pharmaceutical Science
  • Organic Chemistry

Cite this

Park, Jin Woo ; Kim, YoungSoo ; Lee, Kee Jung ; Kim, Dong Jin. / Novel cyanine dyes with vinylsulfone group for labeling biomolecules. In: Bioconjugate Chemistry. 2012 ; Vol. 23, No. 3. pp. 350-362.
@article{46fddcd53831494d96628668a17ee316,
title = "Novel cyanine dyes with vinylsulfone group for labeling biomolecules",
abstract = "Novel vinylsulfone cyanine dyes (em. 550-850 nm) were designed and synthesized for fluorescent labeling of biomolecules via 1,2-addition reaction in aqueous conditions. Due to the virtue of chemical structures of both fluorophore and reactive group, these dyes could be significantly stable and reactive in various aqueous/organic conditions. A wide variety of pH, temperature, buffer concentration, and protein were tested for the optimal labeling condition.",
author = "Park, {Jin Woo} and YoungSoo Kim and Lee, {Kee Jung} and Kim, {Dong Jin}",
year = "2012",
month = "3",
day = "21",
doi = "10.1021/bc200232d",
language = "English",
volume = "23",
pages = "350--362",
journal = "Bioconjugate Chemistry",
issn = "1043-1802",
publisher = "American Chemical Society",
number = "3",

}

Novel cyanine dyes with vinylsulfone group for labeling biomolecules. / Park, Jin Woo; Kim, YoungSoo; Lee, Kee Jung; Kim, Dong Jin.

In: Bioconjugate Chemistry, Vol. 23, No. 3, 21.03.2012, p. 350-362.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Novel cyanine dyes with vinylsulfone group for labeling biomolecules

AU - Park, Jin Woo

AU - Kim, YoungSoo

AU - Lee, Kee Jung

AU - Kim, Dong Jin

PY - 2012/3/21

Y1 - 2012/3/21

N2 - Novel vinylsulfone cyanine dyes (em. 550-850 nm) were designed and synthesized for fluorescent labeling of biomolecules via 1,2-addition reaction in aqueous conditions. Due to the virtue of chemical structures of both fluorophore and reactive group, these dyes could be significantly stable and reactive in various aqueous/organic conditions. A wide variety of pH, temperature, buffer concentration, and protein were tested for the optimal labeling condition.

AB - Novel vinylsulfone cyanine dyes (em. 550-850 nm) were designed and synthesized for fluorescent labeling of biomolecules via 1,2-addition reaction in aqueous conditions. Due to the virtue of chemical structures of both fluorophore and reactive group, these dyes could be significantly stable and reactive in various aqueous/organic conditions. A wide variety of pH, temperature, buffer concentration, and protein were tested for the optimal labeling condition.

UR - http://www.scopus.com/inward/record.url?scp=84863390893&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84863390893&partnerID=8YFLogxK

U2 - 10.1021/bc200232d

DO - 10.1021/bc200232d

M3 - Article

C2 - 22243689

AN - SCOPUS:84863390893

VL - 23

SP - 350

EP - 362

JO - Bioconjugate Chemistry

JF - Bioconjugate Chemistry

SN - 1043-1802

IS - 3

ER -