TY - JOUR
T1 - Novel functions of lipid-binding protein 5 in Caenorhabditis elegans fat metabolism
AU - Xu, Mo
AU - Joo, Hyoe Jin
AU - Paik, Young Ki
N1 - Copyright:
Copyright 2012 Elsevier B.V., All rights reserved.
PY - 2011/8/12
Y1 - 2011/8/12
N2 - The lipid-binding protein (LBP) family is conserved from Caenorhabditis elegans to mammals and essential for fatty acid homeostasis. RNAi-mediated knockdown of nine C. elegans lbp family members revealed that lbp-5 regulates fat accumulation. C. elegans LBP-5 bound directly to various fatty acids with varying affinities. lbp-5 expression in nhr-49(nr2041) worms was much lower than in N2 worms. Nhr-49 transcriptional activity also decreased with lbp-5 deletion, suggesting that they may work together as functional partners in fat metabolism. In support of this notion, LBP-5 translocated into nuclei, where it appeared to influence C. elegans NHR-49 target genes involved in energy metabolism. Interestingly, LBP-5 is required for stearic acid-induced transcription of NHR-49 target genes. Thus, this knowledge could help identify therapeutic targets to treat obesity and diseases associated with nematode-host interactions.
AB - The lipid-binding protein (LBP) family is conserved from Caenorhabditis elegans to mammals and essential for fatty acid homeostasis. RNAi-mediated knockdown of nine C. elegans lbp family members revealed that lbp-5 regulates fat accumulation. C. elegans LBP-5 bound directly to various fatty acids with varying affinities. lbp-5 expression in nhr-49(nr2041) worms was much lower than in N2 worms. Nhr-49 transcriptional activity also decreased with lbp-5 deletion, suggesting that they may work together as functional partners in fat metabolism. In support of this notion, LBP-5 translocated into nuclei, where it appeared to influence C. elegans NHR-49 target genes involved in energy metabolism. Interestingly, LBP-5 is required for stearic acid-induced transcription of NHR-49 target genes. Thus, this knowledge could help identify therapeutic targets to treat obesity and diseases associated with nematode-host interactions.
UR - http://www.scopus.com/inward/record.url?scp=80051516676&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=80051516676&partnerID=8YFLogxK
U2 - 10.1074/jbc.M111.227165
DO - 10.1074/jbc.M111.227165
M3 - Article
C2 - 21697096
AN - SCOPUS:80051516676
VL - 286
SP - 28111
EP - 28118
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
SN - 0021-9258
IS - 32
ER -