Novel functions of lipid-binding protein 5 in Caenorhabditis elegans fat metabolism

Mo Xu, Hyoe Jin Joo, Young Ki Paik

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The lipid-binding protein (LBP) family is conserved from Caenorhabditis elegans to mammals and essential for fatty acid homeostasis. RNAi-mediated knockdown of nine C. elegans lbp family members revealed that lbp-5 regulates fat accumulation. C. elegans LBP-5 bound directly to various fatty acids with varying affinities. lbp-5 expression in nhr-49(nr2041) worms was much lower than in N2 worms. Nhr-49 transcriptional activity also decreased with lbp-5 deletion, suggesting that they may work together as functional partners in fat metabolism. In support of this notion, LBP-5 translocated into nuclei, where it appeared to influence C. elegans NHR-49 target genes involved in energy metabolism. Interestingly, LBP-5 is required for stearic acid-induced transcription of NHR-49 target genes. Thus, this knowledge could help identify therapeutic targets to treat obesity and diseases associated with nematode-host interactions.

Original languageEnglish
Pages (from-to)28111-28118
Number of pages8
JournalJournal of Biological Chemistry
Volume286
Issue number32
DOIs
Publication statusPublished - 2011 Aug 12

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All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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