Novel polymorphic region of the rpoB gene containing Mycobacterium species-specific sequences and its use in identification of mycobacteria

Hyeyoung Lee, Hye Eun Bang, Gill Han Bai, Sang Nae Cho

Research output: Contribution to journalArticlepeer-review

46 Citations (Scopus)

Abstract

Sequence analysis of a specific region of the mycobacterium rpoB gene in 35 mycobacterial strains representing 26 different mycobacterial species of clinical importance showed that there exists a highly polymorphic region. Based on the sequences of the polymorphic region, the oligonucleotide probes of 14 mycobacterial species with relatively high clinical importance were designed and shown to be specific to their corresponding mycobacterial species by dot blot hybridization. The results showed that the probes designed in this study are highly specific to each mycobacterial species, which suggests that these sequences may be useful for the species identification of mycobacteria.

Original languageEnglish
Pages (from-to)2213-2218
Number of pages6
JournalJournal of Clinical Microbiology
Volume41
Issue number5
DOIs
Publication statusPublished - 2003 May 1

All Science Journal Classification (ASJC) codes

  • Microbiology (medical)

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