Novel porous matrix of hyaluronic acid for the three-dimensional culture of chondrocytes

Jeong Yeon Kang, Chung Wook Chung, Jong Hyuk Sung, Byung Soon Park, Je Yong Choi, Seung Jin Lee, Byung Chul Choi, Chang Koo Shim, Suk Jae Chung, Dae Duk Kim

Research output: Contribution to journalArticle

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Abstract

A novel three-dimensional (3D) scaffold of chemically unmodified hyaluronic acid (HA) with minimum cross-linkage was developed for the culture of chondrocytes, thereby to promote cartilage repair. The porous structure of the scaffold was observed by scanning electron microscopy (SEM), and the pore size was controlled by fabrication conditions including swelling time and composition of the HA matrix. Rabbit primary chondrocytes and human chondrocytic cell lines (C-20/A4) were cultured in the HA matrix to investigate whether they can be applied to construct the cartilage tissue in vitro. The chondrocytes retained chondrocytic spherical morphology in this HA matrix. Moreover, results from the MTT assay showed good cellular viability within the HA matrix; optical density increased for up to 28 days, demonstrating that the cells continued to proliferate inside the HA matrix. Phenotypic analysis (RT-PCR, Alcian blue staining and quantification of s-GAG) showed that chondrocytes, when three-dimensionally cultured within the HA matrix, expressed transcripts encoding collagen type II and aggrecan, and produced sulfated glycosaminoglycans (s-GAG), indicating chondrogenic differentiation. The new HA matrix therefore appears as a potentially promising scaffold for the three-dimensional culture of chondrocytes for cartilage tissue engineering.

Original languageEnglish
Pages (from-to)114-120
Number of pages7
JournalInternational Journal of Pharmaceutics
Volume369
Issue number1-2
DOIs
Publication statusPublished - 2009 Mar 18

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Hyaluronic Acid
Chondrocytes
A73025
Cartilage
Aggrecans
Alcian Blue
Collagen Type II
Tissue Engineering
Electron Scanning Microscopy
Staining and Labeling
Rabbits
Cell Line
Polymerase Chain Reaction

All Science Journal Classification (ASJC) codes

  • Pharmaceutical Science

Cite this

Kang, Jeong Yeon ; Chung, Chung Wook ; Sung, Jong Hyuk ; Park, Byung Soon ; Choi, Je Yong ; Lee, Seung Jin ; Choi, Byung Chul ; Shim, Chang Koo ; Chung, Suk Jae ; Kim, Dae Duk. / Novel porous matrix of hyaluronic acid for the three-dimensional culture of chondrocytes. In: International Journal of Pharmaceutics. 2009 ; Vol. 369, No. 1-2. pp. 114-120.
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abstract = "A novel three-dimensional (3D) scaffold of chemically unmodified hyaluronic acid (HA) with minimum cross-linkage was developed for the culture of chondrocytes, thereby to promote cartilage repair. The porous structure of the scaffold was observed by scanning electron microscopy (SEM), and the pore size was controlled by fabrication conditions including swelling time and composition of the HA matrix. Rabbit primary chondrocytes and human chondrocytic cell lines (C-20/A4) were cultured in the HA matrix to investigate whether they can be applied to construct the cartilage tissue in vitro. The chondrocytes retained chondrocytic spherical morphology in this HA matrix. Moreover, results from the MTT assay showed good cellular viability within the HA matrix; optical density increased for up to 28 days, demonstrating that the cells continued to proliferate inside the HA matrix. Phenotypic analysis (RT-PCR, Alcian blue staining and quantification of s-GAG) showed that chondrocytes, when three-dimensionally cultured within the HA matrix, expressed transcripts encoding collagen type II and aggrecan, and produced sulfated glycosaminoglycans (s-GAG), indicating chondrogenic differentiation. The new HA matrix therefore appears as a potentially promising scaffold for the three-dimensional culture of chondrocytes for cartilage tissue engineering.",
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Kang, JY, Chung, CW, Sung, JH, Park, BS, Choi, JY, Lee, SJ, Choi, BC, Shim, CK, Chung, SJ & Kim, DD 2009, 'Novel porous matrix of hyaluronic acid for the three-dimensional culture of chondrocytes', International Journal of Pharmaceutics, vol. 369, no. 1-2, pp. 114-120. https://doi.org/10.1016/j.ijpharm.2008.11.008

Novel porous matrix of hyaluronic acid for the three-dimensional culture of chondrocytes. / Kang, Jeong Yeon; Chung, Chung Wook; Sung, Jong Hyuk; Park, Byung Soon; Choi, Je Yong; Lee, Seung Jin; Choi, Byung Chul; Shim, Chang Koo; Chung, Suk Jae; Kim, Dae Duk.

In: International Journal of Pharmaceutics, Vol. 369, No. 1-2, 18.03.2009, p. 114-120.

Research output: Contribution to journalArticle

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AU - Lee, Seung Jin

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AU - Shim, Chang Koo

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AU - Kim, Dae Duk

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N2 - A novel three-dimensional (3D) scaffold of chemically unmodified hyaluronic acid (HA) with minimum cross-linkage was developed for the culture of chondrocytes, thereby to promote cartilage repair. The porous structure of the scaffold was observed by scanning electron microscopy (SEM), and the pore size was controlled by fabrication conditions including swelling time and composition of the HA matrix. Rabbit primary chondrocytes and human chondrocytic cell lines (C-20/A4) were cultured in the HA matrix to investigate whether they can be applied to construct the cartilage tissue in vitro. The chondrocytes retained chondrocytic spherical morphology in this HA matrix. Moreover, results from the MTT assay showed good cellular viability within the HA matrix; optical density increased for up to 28 days, demonstrating that the cells continued to proliferate inside the HA matrix. Phenotypic analysis (RT-PCR, Alcian blue staining and quantification of s-GAG) showed that chondrocytes, when three-dimensionally cultured within the HA matrix, expressed transcripts encoding collagen type II and aggrecan, and produced sulfated glycosaminoglycans (s-GAG), indicating chondrogenic differentiation. The new HA matrix therefore appears as a potentially promising scaffold for the three-dimensional culture of chondrocytes for cartilage tissue engineering.

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