Nuclear import of human histone lysine-specific demethylase LSD1

Yanhua Jin, Tae Young Kim, Min Seong Kim, Min Aeh Kim, Su Hyung Park, Yeun Kyu Jang

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Abstract

Upregulation and nuclear retention of the human histone demethylase LSD1 are correlated with aggressiveness and poor outcome of several cancer types, but the molecular mechanism of LSD1 nuclear import remains unclear. Here, we found that the N-terminal flexible region of LSD1 contains a nuclear localization signal (NLS), 112 RRKRAK 117 . Mutation or deletion of the NLS completely abolished the nuclear import of LSD1, suggesting the motif is a bona fide NLS. More importantly, our GST pull-down assay showed that LSD1 physically interacts with three proteins of importin α family. In addition, our data suggest that the nuclear localization of LSD1 via the NLS is not a cell-type specific event. Thus, these findings demonstrate for the first time that the NLS motif within the N-terminal flexible domain of LSD1 is critical for its nuclear localization via interaction with importin α proteins.

Original languageEnglish
Pages (from-to)305-313
Number of pages9
JournalJournal of Biochemistry
Volume156
Issue number6
DOIs
Publication statusPublished - 2014 Dec 1

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All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology

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