Nuclear loss of DNA repair Ku proteins and c-myc expression in apoptosis of cerulein-stimulated pancreatic acinar cells

J. H. Yu, K. H. Kim, Hye Young Kim

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Cerulein induces oxidative stress and an acute, edematous form of pancreatitis. Cell death linked to oxidative DNA damage has been implicated in acute pancreatitis. During DNA damage, DNA repair proteins, Ku70 and Ku80, prevent cell death but severe DNA damage triggers apoptosis. This study aims to investigate the role of Ku70 and Ku80 on apoptotic cell death, induced by cerulein in pancreatic acinar AR42J cells. We examined Ku expression, Ku-DNA binding activity, cell viability and mRNA expression of c-myc of the cells stimulated with cerulein. As a result, cerulein induced decrease in nuclear Ku70 and Ku80 with increase in cytoplasmic Ku proteins. Cerulein decreased Ku-DNA binding activity in parallel with increase in cell death and mRNA expression of c-myc. Conclusively, nuclear loss of Ku70 and Ku80 may cause the loss of defense against DNA damage and apoptosis in pancreatic acinar cells stimulated with cerulein.

Original languageEnglish
Pages (from-to)282-287
Number of pages6
JournalInflammopharmacology
Volume15
Issue number6
DOIs
Publication statusPublished - 2007 Dec 1

Fingerprint

Ceruletide
Acinar Cells
DNA Repair
Apoptosis
DNA Damage
Cell Death
Pancreatitis
Messenger RNA
DNA
Ku Autoantigen
Cell Survival
Oxidative Stress
Proteins

All Science Journal Classification (ASJC) codes

  • Immunology
  • Pharmacology
  • Pharmacology (medical)

Cite this

@article{9daf02663dd44271acb2d9f6d80c7d1d,
title = "Nuclear loss of DNA repair Ku proteins and c-myc expression in apoptosis of cerulein-stimulated pancreatic acinar cells",
abstract = "Cerulein induces oxidative stress and an acute, edematous form of pancreatitis. Cell death linked to oxidative DNA damage has been implicated in acute pancreatitis. During DNA damage, DNA repair proteins, Ku70 and Ku80, prevent cell death but severe DNA damage triggers apoptosis. This study aims to investigate the role of Ku70 and Ku80 on apoptotic cell death, induced by cerulein in pancreatic acinar AR42J cells. We examined Ku expression, Ku-DNA binding activity, cell viability and mRNA expression of c-myc of the cells stimulated with cerulein. As a result, cerulein induced decrease in nuclear Ku70 and Ku80 with increase in cytoplasmic Ku proteins. Cerulein decreased Ku-DNA binding activity in parallel with increase in cell death and mRNA expression of c-myc. Conclusively, nuclear loss of Ku70 and Ku80 may cause the loss of defense against DNA damage and apoptosis in pancreatic acinar cells stimulated with cerulein.",
author = "Yu, {J. H.} and Kim, {K. H.} and Kim, {Hye Young}",
year = "2007",
month = "12",
day = "1",
doi = "10.1007/s10787-007-1588-y",
language = "English",
volume = "15",
pages = "282--287",
journal = "Inflammopharmacology",
issn = "0925-4692",
publisher = "Birkhauser Verlag Basel",
number = "6",

}

Nuclear loss of DNA repair Ku proteins and c-myc expression in apoptosis of cerulein-stimulated pancreatic acinar cells. / Yu, J. H.; Kim, K. H.; Kim, Hye Young.

In: Inflammopharmacology, Vol. 15, No. 6, 01.12.2007, p. 282-287.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Nuclear loss of DNA repair Ku proteins and c-myc expression in apoptosis of cerulein-stimulated pancreatic acinar cells

AU - Yu, J. H.

AU - Kim, K. H.

AU - Kim, Hye Young

PY - 2007/12/1

Y1 - 2007/12/1

N2 - Cerulein induces oxidative stress and an acute, edematous form of pancreatitis. Cell death linked to oxidative DNA damage has been implicated in acute pancreatitis. During DNA damage, DNA repair proteins, Ku70 and Ku80, prevent cell death but severe DNA damage triggers apoptosis. This study aims to investigate the role of Ku70 and Ku80 on apoptotic cell death, induced by cerulein in pancreatic acinar AR42J cells. We examined Ku expression, Ku-DNA binding activity, cell viability and mRNA expression of c-myc of the cells stimulated with cerulein. As a result, cerulein induced decrease in nuclear Ku70 and Ku80 with increase in cytoplasmic Ku proteins. Cerulein decreased Ku-DNA binding activity in parallel with increase in cell death and mRNA expression of c-myc. Conclusively, nuclear loss of Ku70 and Ku80 may cause the loss of defense against DNA damage and apoptosis in pancreatic acinar cells stimulated with cerulein.

AB - Cerulein induces oxidative stress and an acute, edematous form of pancreatitis. Cell death linked to oxidative DNA damage has been implicated in acute pancreatitis. During DNA damage, DNA repair proteins, Ku70 and Ku80, prevent cell death but severe DNA damage triggers apoptosis. This study aims to investigate the role of Ku70 and Ku80 on apoptotic cell death, induced by cerulein in pancreatic acinar AR42J cells. We examined Ku expression, Ku-DNA binding activity, cell viability and mRNA expression of c-myc of the cells stimulated with cerulein. As a result, cerulein induced decrease in nuclear Ku70 and Ku80 with increase in cytoplasmic Ku proteins. Cerulein decreased Ku-DNA binding activity in parallel with increase in cell death and mRNA expression of c-myc. Conclusively, nuclear loss of Ku70 and Ku80 may cause the loss of defense against DNA damage and apoptosis in pancreatic acinar cells stimulated with cerulein.

UR - http://www.scopus.com/inward/record.url?scp=38849136488&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=38849136488&partnerID=8YFLogxK

U2 - 10.1007/s10787-007-1588-y

DO - 10.1007/s10787-007-1588-y

M3 - Article

C2 - 18236021

AN - SCOPUS:38849136488

VL - 15

SP - 282

EP - 287

JO - Inflammopharmacology

JF - Inflammopharmacology

SN - 0925-4692

IS - 6

ER -