Nuclear trapping controls the position-dependent localization of CAPRICE in the root epidermis of arabidopsis

Yeon Hee Kang, Sang Kee Song, John Schiefelbein, Myeong Min Lee

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Cell fate determination and differentiation are central processes in the development of multicellular organisms, and the Arabidopsis (Arabidopsis thaliana) root epidermis provides a model system to study the molecular basis of these processes. A lateral inhibition mechanism mediated by an R3 single-repeat MYB protein, CAPRICE (CPC), has been proposed to explain the specification of the two types of root epidermal cells (hair cells and nonhair cells). However, it is not clear how CPC acts preferentially in the H-position cells, rather than the N-position cells, where its gene is expressed. To explore this issue, we examined the effect of misexpressed CPC on cell fate specification and CPC localization in the root epidermis. We show that CPC is able to move readily within the root epidermis when its expression level is high and that CPC can induce the hair cell fate in a cell-autonomous manner. We provide evidence that CPC is capable of moving from the stele tissue in the center of the root to the outermost epidermal layer, where it can induce the hair cell fate. In addition, we show that CPC protein accumulates primarily in the nuclei of H-position cells in the early meristematic region, and this localization requires the H-cell-expressed ENHANCER OF GLABRA3 (EGL3) basic helix-loop-helix transcription factor. These results suggest that cell-cell movement of CPC occurs readily within the meristematic region of the root and that EGL3 preferentially traps the CPC protein in the H-position cells of the epidermis.

Original languageEnglish
Pages (from-to)193-204
Number of pages12
JournalPlant physiology
Volume163
Issue number1
DOIs
Publication statusPublished - 2013 Sep 1

Fingerprint

epidermis (animal)
Arabidopsis
Epidermis
trapping
cells
hairs
basic helix-loop-helix transcription factors
Basic Helix-Loop-Helix Transcription Factors
stele
Proteins
proteins
cell movement
Cell Movement

All Science Journal Classification (ASJC) codes

  • Physiology
  • Genetics
  • Plant Science

Cite this

Kang, Yeon Hee ; Song, Sang Kee ; Schiefelbein, John ; Lee, Myeong Min. / Nuclear trapping controls the position-dependent localization of CAPRICE in the root epidermis of arabidopsis. In: Plant physiology. 2013 ; Vol. 163, No. 1. pp. 193-204.
@article{08bd6c2c06184a3cac6a8cf244d59b1e,
title = "Nuclear trapping controls the position-dependent localization of CAPRICE in the root epidermis of arabidopsis",
abstract = "Cell fate determination and differentiation are central processes in the development of multicellular organisms, and the Arabidopsis (Arabidopsis thaliana) root epidermis provides a model system to study the molecular basis of these processes. A lateral inhibition mechanism mediated by an R3 single-repeat MYB protein, CAPRICE (CPC), has been proposed to explain the specification of the two types of root epidermal cells (hair cells and nonhair cells). However, it is not clear how CPC acts preferentially in the H-position cells, rather than the N-position cells, where its gene is expressed. To explore this issue, we examined the effect of misexpressed CPC on cell fate specification and CPC localization in the root epidermis. We show that CPC is able to move readily within the root epidermis when its expression level is high and that CPC can induce the hair cell fate in a cell-autonomous manner. We provide evidence that CPC is capable of moving from the stele tissue in the center of the root to the outermost epidermal layer, where it can induce the hair cell fate. In addition, we show that CPC protein accumulates primarily in the nuclei of H-position cells in the early meristematic region, and this localization requires the H-cell-expressed ENHANCER OF GLABRA3 (EGL3) basic helix-loop-helix transcription factor. These results suggest that cell-cell movement of CPC occurs readily within the meristematic region of the root and that EGL3 preferentially traps the CPC protein in the H-position cells of the epidermis.",
author = "Kang, {Yeon Hee} and Song, {Sang Kee} and John Schiefelbein and Lee, {Myeong Min}",
year = "2013",
month = "9",
day = "1",
doi = "10.1104/pp.113.221028",
language = "English",
volume = "163",
pages = "193--204",
journal = "Plant Physiology",
issn = "0032-0889",
publisher = "American Society of Plant Biologists",
number = "1",

}

Nuclear trapping controls the position-dependent localization of CAPRICE in the root epidermis of arabidopsis. / Kang, Yeon Hee; Song, Sang Kee; Schiefelbein, John; Lee, Myeong Min.

In: Plant physiology, Vol. 163, No. 1, 01.09.2013, p. 193-204.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Nuclear trapping controls the position-dependent localization of CAPRICE in the root epidermis of arabidopsis

AU - Kang, Yeon Hee

AU - Song, Sang Kee

AU - Schiefelbein, John

AU - Lee, Myeong Min

PY - 2013/9/1

Y1 - 2013/9/1

N2 - Cell fate determination and differentiation are central processes in the development of multicellular organisms, and the Arabidopsis (Arabidopsis thaliana) root epidermis provides a model system to study the molecular basis of these processes. A lateral inhibition mechanism mediated by an R3 single-repeat MYB protein, CAPRICE (CPC), has been proposed to explain the specification of the two types of root epidermal cells (hair cells and nonhair cells). However, it is not clear how CPC acts preferentially in the H-position cells, rather than the N-position cells, where its gene is expressed. To explore this issue, we examined the effect of misexpressed CPC on cell fate specification and CPC localization in the root epidermis. We show that CPC is able to move readily within the root epidermis when its expression level is high and that CPC can induce the hair cell fate in a cell-autonomous manner. We provide evidence that CPC is capable of moving from the stele tissue in the center of the root to the outermost epidermal layer, where it can induce the hair cell fate. In addition, we show that CPC protein accumulates primarily in the nuclei of H-position cells in the early meristematic region, and this localization requires the H-cell-expressed ENHANCER OF GLABRA3 (EGL3) basic helix-loop-helix transcription factor. These results suggest that cell-cell movement of CPC occurs readily within the meristematic region of the root and that EGL3 preferentially traps the CPC protein in the H-position cells of the epidermis.

AB - Cell fate determination and differentiation are central processes in the development of multicellular organisms, and the Arabidopsis (Arabidopsis thaliana) root epidermis provides a model system to study the molecular basis of these processes. A lateral inhibition mechanism mediated by an R3 single-repeat MYB protein, CAPRICE (CPC), has been proposed to explain the specification of the two types of root epidermal cells (hair cells and nonhair cells). However, it is not clear how CPC acts preferentially in the H-position cells, rather than the N-position cells, where its gene is expressed. To explore this issue, we examined the effect of misexpressed CPC on cell fate specification and CPC localization in the root epidermis. We show that CPC is able to move readily within the root epidermis when its expression level is high and that CPC can induce the hair cell fate in a cell-autonomous manner. We provide evidence that CPC is capable of moving from the stele tissue in the center of the root to the outermost epidermal layer, where it can induce the hair cell fate. In addition, we show that CPC protein accumulates primarily in the nuclei of H-position cells in the early meristematic region, and this localization requires the H-cell-expressed ENHANCER OF GLABRA3 (EGL3) basic helix-loop-helix transcription factor. These results suggest that cell-cell movement of CPC occurs readily within the meristematic region of the root and that EGL3 preferentially traps the CPC protein in the H-position cells of the epidermis.

UR - http://www.scopus.com/inward/record.url?scp=84883238386&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84883238386&partnerID=8YFLogxK

U2 - 10.1104/pp.113.221028

DO - 10.1104/pp.113.221028

M3 - Article

C2 - 23832626

AN - SCOPUS:84883238386

VL - 163

SP - 193

EP - 204

JO - Plant Physiology

JF - Plant Physiology

SN - 0032-0889

IS - 1

ER -