Nucleoporin MOS7/Nup88 is required for mitosis in gametogenesis and seed development in Arabidopsis

Guen Tae Park, Jennifer M. Frost, Jin Sup Park, Tae Ho Kim, Jong Seob Lee, Sung Aeong Oh, David Twell, Janie Sue Brooks, Robert L. Fischer, Yeonhee Choi

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Angiosperm reproduction is characterized by alternate diploid sporophytic and haploid gametophytic generations. Gametogenesis shares similarities with that of animals except for the formation of the gametophyte, whereby haploid cells undergo several rounds of postmeiotic mitosis to form gametes and the accessory cells required for successful reproduction. The mechanisms regulating gametophyte development in angiosperms are incompletely understood. Here, we show that the nucleoporin Nup88-homolog MOS7 (Modifier of Snc1,7) plays a crucial role in mitosis during both male and female gametophyte formation in Arabidopsis thaliana. Using a mutagenesis screen, we identify the mos7-5 mutant allele, which causes ovule and pollen abortion in MOS7/mos7-5 heterozygous plants, and preglobular stage embryonic lethality in homozygous mos7-5 seeds. During interphase, we show that MOS7 is localized to the nuclear membrane but, like many nucleoporins, is associated with the spindle apparatus during mitosis. We detect interactions between MOS7 and several nucleoporins known to control spindle dynamics, and find that in pollen from MOS7/mos7-5 heterozygotes, abortion is accompanied by a failure of spindle formation, cell fate specification, and phragmoplast activity. Most intriguingly, we show that following gamete formation by MOS7/mos7-5 heterozygous spores, inheritance of either the MOS7 or the mos7-5 allele by a given gamete does not correlate with its respective survival or abortion. Instead, we suggest a model whereby MOS7, which is highly expressed in the Pollen- and Megaspore Mother Cells, enacts a dosage-limiting effect on the gametes to enable their progression through subsequent mitoses.

Original languageEnglish
Pages (from-to)18393-18398
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume111
Issue number51
DOIs
Publication statusPublished - 2014 Dec 23

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Nuclear Pore Complex Proteins
Gametogenesis
Arabidopsis
Mitosis
Germ Cells
Seeds
Pollen
Angiosperms
Haploidy
Reproduction
Alleles
Ovule
Spindle Apparatus
Interphase
Nuclear Envelope
Heterozygote
Spores
Diploidy
Mutagenesis
Stem Cells

All Science Journal Classification (ASJC) codes

  • General

Cite this

Park, Guen Tae ; Frost, Jennifer M. ; Park, Jin Sup ; Kim, Tae Ho ; Lee, Jong Seob ; Oh, Sung Aeong ; Twell, David ; Brooks, Janie Sue ; Fischer, Robert L. ; Choi, Yeonhee. / Nucleoporin MOS7/Nup88 is required for mitosis in gametogenesis and seed development in Arabidopsis. In: Proceedings of the National Academy of Sciences of the United States of America. 2014 ; Vol. 111, No. 51. pp. 18393-18398.
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title = "Nucleoporin MOS7/Nup88 is required for mitosis in gametogenesis and seed development in Arabidopsis",
abstract = "Angiosperm reproduction is characterized by alternate diploid sporophytic and haploid gametophytic generations. Gametogenesis shares similarities with that of animals except for the formation of the gametophyte, whereby haploid cells undergo several rounds of postmeiotic mitosis to form gametes and the accessory cells required for successful reproduction. The mechanisms regulating gametophyte development in angiosperms are incompletely understood. Here, we show that the nucleoporin Nup88-homolog MOS7 (Modifier of Snc1,7) plays a crucial role in mitosis during both male and female gametophyte formation in Arabidopsis thaliana. Using a mutagenesis screen, we identify the mos7-5 mutant allele, which causes ovule and pollen abortion in MOS7/mos7-5 heterozygous plants, and preglobular stage embryonic lethality in homozygous mos7-5 seeds. During interphase, we show that MOS7 is localized to the nuclear membrane but, like many nucleoporins, is associated with the spindle apparatus during mitosis. We detect interactions between MOS7 and several nucleoporins known to control spindle dynamics, and find that in pollen from MOS7/mos7-5 heterozygotes, abortion is accompanied by a failure of spindle formation, cell fate specification, and phragmoplast activity. Most intriguingly, we show that following gamete formation by MOS7/mos7-5 heterozygous spores, inheritance of either the MOS7 or the mos7-5 allele by a given gamete does not correlate with its respective survival or abortion. Instead, we suggest a model whereby MOS7, which is highly expressed in the Pollen- and Megaspore Mother Cells, enacts a dosage-limiting effect on the gametes to enable their progression through subsequent mitoses.",
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Nucleoporin MOS7/Nup88 is required for mitosis in gametogenesis and seed development in Arabidopsis. / Park, Guen Tae; Frost, Jennifer M.; Park, Jin Sup; Kim, Tae Ho; Lee, Jong Seob; Oh, Sung Aeong; Twell, David; Brooks, Janie Sue; Fischer, Robert L.; Choi, Yeonhee.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 111, No. 51, 23.12.2014, p. 18393-18398.

Research output: Contribution to journalArticle

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T1 - Nucleoporin MOS7/Nup88 is required for mitosis in gametogenesis and seed development in Arabidopsis

AU - Park, Guen Tae

AU - Frost, Jennifer M.

AU - Park, Jin Sup

AU - Kim, Tae Ho

AU - Lee, Jong Seob

AU - Oh, Sung Aeong

AU - Twell, David

AU - Brooks, Janie Sue

AU - Fischer, Robert L.

AU - Choi, Yeonhee

PY - 2014/12/23

Y1 - 2014/12/23

N2 - Angiosperm reproduction is characterized by alternate diploid sporophytic and haploid gametophytic generations. Gametogenesis shares similarities with that of animals except for the formation of the gametophyte, whereby haploid cells undergo several rounds of postmeiotic mitosis to form gametes and the accessory cells required for successful reproduction. The mechanisms regulating gametophyte development in angiosperms are incompletely understood. Here, we show that the nucleoporin Nup88-homolog MOS7 (Modifier of Snc1,7) plays a crucial role in mitosis during both male and female gametophyte formation in Arabidopsis thaliana. Using a mutagenesis screen, we identify the mos7-5 mutant allele, which causes ovule and pollen abortion in MOS7/mos7-5 heterozygous plants, and preglobular stage embryonic lethality in homozygous mos7-5 seeds. During interphase, we show that MOS7 is localized to the nuclear membrane but, like many nucleoporins, is associated with the spindle apparatus during mitosis. We detect interactions between MOS7 and several nucleoporins known to control spindle dynamics, and find that in pollen from MOS7/mos7-5 heterozygotes, abortion is accompanied by a failure of spindle formation, cell fate specification, and phragmoplast activity. Most intriguingly, we show that following gamete formation by MOS7/mos7-5 heterozygous spores, inheritance of either the MOS7 or the mos7-5 allele by a given gamete does not correlate with its respective survival or abortion. Instead, we suggest a model whereby MOS7, which is highly expressed in the Pollen- and Megaspore Mother Cells, enacts a dosage-limiting effect on the gametes to enable their progression through subsequent mitoses.

AB - Angiosperm reproduction is characterized by alternate diploid sporophytic and haploid gametophytic generations. Gametogenesis shares similarities with that of animals except for the formation of the gametophyte, whereby haploid cells undergo several rounds of postmeiotic mitosis to form gametes and the accessory cells required for successful reproduction. The mechanisms regulating gametophyte development in angiosperms are incompletely understood. Here, we show that the nucleoporin Nup88-homolog MOS7 (Modifier of Snc1,7) plays a crucial role in mitosis during both male and female gametophyte formation in Arabidopsis thaliana. Using a mutagenesis screen, we identify the mos7-5 mutant allele, which causes ovule and pollen abortion in MOS7/mos7-5 heterozygous plants, and preglobular stage embryonic lethality in homozygous mos7-5 seeds. During interphase, we show that MOS7 is localized to the nuclear membrane but, like many nucleoporins, is associated with the spindle apparatus during mitosis. We detect interactions between MOS7 and several nucleoporins known to control spindle dynamics, and find that in pollen from MOS7/mos7-5 heterozygotes, abortion is accompanied by a failure of spindle formation, cell fate specification, and phragmoplast activity. Most intriguingly, we show that following gamete formation by MOS7/mos7-5 heterozygous spores, inheritance of either the MOS7 or the mos7-5 allele by a given gamete does not correlate with its respective survival or abortion. Instead, we suggest a model whereby MOS7, which is highly expressed in the Pollen- and Megaspore Mother Cells, enacts a dosage-limiting effect on the gametes to enable their progression through subsequent mitoses.

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