Nucleotide and primary sequence of a major rice prolamine

Woo Taek Kim, Thomas W. Okita

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

A recombinant cDNA clone encoding a major rice seed storage prolamine was isolated by antibody screening of a cDNA λgt 11 library. This clone contained a single open reading frame encoding a putative rice prolamine precursor (Mr17 300). In contrast to other cereal prolamines, the primary sequence of the rice prolamine was devoid of any major tandem repetitive sequences, a feature prevalent in all cereal prolamines studied to date. No significant homology was detected between the rice prolamine and other cereal prolamines, indicating that the rice gene evolved from unique ancestral DNA segments.

Original languageEnglish
Pages (from-to)308-310
Number of pages3
JournalFEBS Letters
Volume231
Issue number2
DOIs
Publication statusPublished - 1988 Apr 25

Fingerprint

Phenylpropanolamine
Nucleotides
Complementary DNA
Clone Cells
Nucleic Acid Repetitive Sequences
Oryza
Open Reading Frames
Libraries
Seed
Seeds
Screening
Genes
Antibodies
DNA

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Kim, Woo Taek ; Okita, Thomas W. / Nucleotide and primary sequence of a major rice prolamine. In: FEBS Letters. 1988 ; Vol. 231, No. 2. pp. 308-310.
@article{4f1467b240804958a19956134a8a6ba0,
title = "Nucleotide and primary sequence of a major rice prolamine",
abstract = "A recombinant cDNA clone encoding a major rice seed storage prolamine was isolated by antibody screening of a cDNA λgt 11 library. This clone contained a single open reading frame encoding a putative rice prolamine precursor (Mr17 300). In contrast to other cereal prolamines, the primary sequence of the rice prolamine was devoid of any major tandem repetitive sequences, a feature prevalent in all cereal prolamines studied to date. No significant homology was detected between the rice prolamine and other cereal prolamines, indicating that the rice gene evolved from unique ancestral DNA segments.",
author = "Kim, {Woo Taek} and Okita, {Thomas W.}",
year = "1988",
month = "4",
day = "25",
doi = "10.1016/0014-5793(88)80839-1",
language = "English",
volume = "231",
pages = "308--310",
journal = "FEBS Letters",
issn = "0014-5793",
publisher = "Elsevier",
number = "2",

}

Nucleotide and primary sequence of a major rice prolamine. / Kim, Woo Taek; Okita, Thomas W.

In: FEBS Letters, Vol. 231, No. 2, 25.04.1988, p. 308-310.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Nucleotide and primary sequence of a major rice prolamine

AU - Kim, Woo Taek

AU - Okita, Thomas W.

PY - 1988/4/25

Y1 - 1988/4/25

N2 - A recombinant cDNA clone encoding a major rice seed storage prolamine was isolated by antibody screening of a cDNA λgt 11 library. This clone contained a single open reading frame encoding a putative rice prolamine precursor (Mr17 300). In contrast to other cereal prolamines, the primary sequence of the rice prolamine was devoid of any major tandem repetitive sequences, a feature prevalent in all cereal prolamines studied to date. No significant homology was detected between the rice prolamine and other cereal prolamines, indicating that the rice gene evolved from unique ancestral DNA segments.

AB - A recombinant cDNA clone encoding a major rice seed storage prolamine was isolated by antibody screening of a cDNA λgt 11 library. This clone contained a single open reading frame encoding a putative rice prolamine precursor (Mr17 300). In contrast to other cereal prolamines, the primary sequence of the rice prolamine was devoid of any major tandem repetitive sequences, a feature prevalent in all cereal prolamines studied to date. No significant homology was detected between the rice prolamine and other cereal prolamines, indicating that the rice gene evolved from unique ancestral DNA segments.

UR - http://www.scopus.com/inward/record.url?scp=0024296572&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024296572&partnerID=8YFLogxK

U2 - 10.1016/0014-5793(88)80839-1

DO - 10.1016/0014-5793(88)80839-1

M3 - Article

C2 - 3360138

AN - SCOPUS:0024296572

VL - 231

SP - 308

EP - 310

JO - FEBS Letters

JF - FEBS Letters

SN - 0014-5793

IS - 2

ER -