Nucleotide sequence and structure of the human apolipoprotein E gene

Y. K. Paik, D. J. Chang, C. A. Reardon, G. E. Davies, R. W. Mahley, J. M. Taylor

Research output: Contribution to journalArticle

214 Citations (Scopus)

Abstract

The gene for human apolipoprotein E (apo-E) was selected from a library of cloned genomic DNA by screening with a specific cDNA hybridization probe, and its structure was characterized. The complete nucleotide sequence of the gene as well as 856 nucleotides of the 5' flanking region and 629 nucleotides of the 3' flanking region were determined. Analysis of the sequence showed that the mRNA-encoding region of the apo-E gene consists of four exons separated by three introns. In comparison to the structure of the mRNA, the introns are located in the 5' noncoding region, in the codon for glycine at position -4 of the signal peptide region, and in the codon for arginine at position +61 of the mature protein. The overall lengths of the apo-E gene and its corresponding mRNA are 3597 and 1163 nucleotides, respectively; a mature plasma protein of 299 amino acids is produced by this gene. Examination of the 5' terminus of the gene by S1 nuclease mapping shows apparent multiple transcription initiation sites. The proximal 5' flanking region contains a 'TATA box' element as well as two nearby inverted repeat elements. In addition, there are four Alu family sequences associated with the apo-E gene: an Alu sequence located near each end of the gene and two Alu sequences located in the second intron. This knowledge of the structure permits a molecular approach to characterizing the regulation of the apo-E gene.

Original languageEnglish
Pages (from-to)3445-3449
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume82
Issue number10
DOIs
Publication statusPublished - 1985 Sep 25

Fingerprint

Apolipoproteins E
Genes
Introns
Nucleotides
5' Flanking Region
Codon
Messenger RNA
Alu Elements
3' Flanking Region
TATA Box
Genomic Library
Transcription Initiation Site
Protein Sorting Signals
Glycine
Sequence Analysis
Arginine
Blood Proteins
Exons
Complementary DNA
Amino Acids

All Science Journal Classification (ASJC) codes

  • General

Cite this

Paik, Y. K. ; Chang, D. J. ; Reardon, C. A. ; Davies, G. E. ; Mahley, R. W. ; Taylor, J. M. / Nucleotide sequence and structure of the human apolipoprotein E gene. In: Proceedings of the National Academy of Sciences of the United States of America. 1985 ; Vol. 82, No. 10. pp. 3445-3449.
@article{b77fb0c4e32f493a9baf98f6052a2cb1,
title = "Nucleotide sequence and structure of the human apolipoprotein E gene",
abstract = "The gene for human apolipoprotein E (apo-E) was selected from a library of cloned genomic DNA by screening with a specific cDNA hybridization probe, and its structure was characterized. The complete nucleotide sequence of the gene as well as 856 nucleotides of the 5' flanking region and 629 nucleotides of the 3' flanking region were determined. Analysis of the sequence showed that the mRNA-encoding region of the apo-E gene consists of four exons separated by three introns. In comparison to the structure of the mRNA, the introns are located in the 5' noncoding region, in the codon for glycine at position -4 of the signal peptide region, and in the codon for arginine at position +61 of the mature protein. The overall lengths of the apo-E gene and its corresponding mRNA are 3597 and 1163 nucleotides, respectively; a mature plasma protein of 299 amino acids is produced by this gene. Examination of the 5' terminus of the gene by S1 nuclease mapping shows apparent multiple transcription initiation sites. The proximal 5' flanking region contains a 'TATA box' element as well as two nearby inverted repeat elements. In addition, there are four Alu family sequences associated with the apo-E gene: an Alu sequence located near each end of the gene and two Alu sequences located in the second intron. This knowledge of the structure permits a molecular approach to characterizing the regulation of the apo-E gene.",
author = "Paik, {Y. K.} and Chang, {D. J.} and Reardon, {C. A.} and Davies, {G. E.} and Mahley, {R. W.} and Taylor, {J. M.}",
year = "1985",
month = "9",
day = "25",
doi = "10.1073/pnas.82.10.3445",
language = "English",
volume = "82",
pages = "3445--3449",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
issn = "0027-8424",
number = "10",

}

Nucleotide sequence and structure of the human apolipoprotein E gene. / Paik, Y. K.; Chang, D. J.; Reardon, C. A.; Davies, G. E.; Mahley, R. W.; Taylor, J. M.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 82, No. 10, 25.09.1985, p. 3445-3449.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Nucleotide sequence and structure of the human apolipoprotein E gene

AU - Paik, Y. K.

AU - Chang, D. J.

AU - Reardon, C. A.

AU - Davies, G. E.

AU - Mahley, R. W.

AU - Taylor, J. M.

PY - 1985/9/25

Y1 - 1985/9/25

N2 - The gene for human apolipoprotein E (apo-E) was selected from a library of cloned genomic DNA by screening with a specific cDNA hybridization probe, and its structure was characterized. The complete nucleotide sequence of the gene as well as 856 nucleotides of the 5' flanking region and 629 nucleotides of the 3' flanking region were determined. Analysis of the sequence showed that the mRNA-encoding region of the apo-E gene consists of four exons separated by three introns. In comparison to the structure of the mRNA, the introns are located in the 5' noncoding region, in the codon for glycine at position -4 of the signal peptide region, and in the codon for arginine at position +61 of the mature protein. The overall lengths of the apo-E gene and its corresponding mRNA are 3597 and 1163 nucleotides, respectively; a mature plasma protein of 299 amino acids is produced by this gene. Examination of the 5' terminus of the gene by S1 nuclease mapping shows apparent multiple transcription initiation sites. The proximal 5' flanking region contains a 'TATA box' element as well as two nearby inverted repeat elements. In addition, there are four Alu family sequences associated with the apo-E gene: an Alu sequence located near each end of the gene and two Alu sequences located in the second intron. This knowledge of the structure permits a molecular approach to characterizing the regulation of the apo-E gene.

AB - The gene for human apolipoprotein E (apo-E) was selected from a library of cloned genomic DNA by screening with a specific cDNA hybridization probe, and its structure was characterized. The complete nucleotide sequence of the gene as well as 856 nucleotides of the 5' flanking region and 629 nucleotides of the 3' flanking region were determined. Analysis of the sequence showed that the mRNA-encoding region of the apo-E gene consists of four exons separated by three introns. In comparison to the structure of the mRNA, the introns are located in the 5' noncoding region, in the codon for glycine at position -4 of the signal peptide region, and in the codon for arginine at position +61 of the mature protein. The overall lengths of the apo-E gene and its corresponding mRNA are 3597 and 1163 nucleotides, respectively; a mature plasma protein of 299 amino acids is produced by this gene. Examination of the 5' terminus of the gene by S1 nuclease mapping shows apparent multiple transcription initiation sites. The proximal 5' flanking region contains a 'TATA box' element as well as two nearby inverted repeat elements. In addition, there are four Alu family sequences associated with the apo-E gene: an Alu sequence located near each end of the gene and two Alu sequences located in the second intron. This knowledge of the structure permits a molecular approach to characterizing the regulation of the apo-E gene.

UR - http://www.scopus.com/inward/record.url?scp=0013070348&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0013070348&partnerID=8YFLogxK

U2 - 10.1073/pnas.82.10.3445

DO - 10.1073/pnas.82.10.3445

M3 - Article

C2 - 2987927

AN - SCOPUS:0013070348

VL - 82

SP - 3445

EP - 3449

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

SN - 0027-8424

IS - 10

ER -