Abstract
Ten patients with hepatitis C virus (HCV) infection (experimental group) were treated with interferon-α (IF-α). Dosage was six million units per day for one week and then three times a week for another six months. Seven HCV-infected patients (control group) did not receive IF-α therapy. The hypervariable region (HVR) of HCV in the sera of patients was amplified by reverse trancription-polymerase chain reaction (RT-PCR), and the variation of amino acid sequence in this region was determined. Serum alanine aminotransferase (ALT) activities in five patients treated for six months with IF-α fell to the normal range, when HCV was not detected in the sera of three patients. The nucleotide sequence variation in HVR of HCV in the sera of five patients who responded well to the IF-α. therapy was relatively less than that in another five patients who did not respond to IF-α therapy and those in the control patients. These results indicate that the effectiveness of IF-α therapy was related to the sequence variation of HVR of HCV. This may have resulted from the selection pressure by humoral antibodies directed to HVR of HCV. It is concluded that the higher rate of sequence variation in HVR of HCV was compatible with a lower degree of effectiveness of IF-α therapy.
| Original language | English |
|---|---|
| Pages (from-to) | 95-102 |
| Number of pages | 8 |
| Journal | Journal of Medical Virology |
| Volume | 49 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 1996 Jun 1 |
Fingerprint
All Science Journal Classification (ASJC) codes
- Virology
- Infectious Diseases
Cite this
}
Nucleotide sequence variation in the hypervariable region of the hepatitis C virus in the sera of chronic hepatitis C patients undergoing controlled interferon-α therapy. / Yeh, Byung Il; Han, KwangHyub; Oh, Seung Hee; Kim, Hyon Suk; Hong, Suk Hyun; Oh, Sang Hwan; Kim, Yoon Soo.
In: Journal of Medical Virology, Vol. 49, No. 2, 01.06.1996, p. 95-102.Research output: Contribution to journal › Article
TY - JOUR
T1 - Nucleotide sequence variation in the hypervariable region of the hepatitis C virus in the sera of chronic hepatitis C patients undergoing controlled interferon-α therapy
AU - Yeh, Byung Il
AU - Han, KwangHyub
AU - Oh, Seung Hee
AU - Kim, Hyon Suk
AU - Hong, Suk Hyun
AU - Oh, Sang Hwan
AU - Kim, Yoon Soo
PY - 1996/6/1
Y1 - 1996/6/1
N2 - Ten patients with hepatitis C virus (HCV) infection (experimental group) were treated with interferon-α (IF-α). Dosage was six million units per day for one week and then three times a week for another six months. Seven HCV-infected patients (control group) did not receive IF-α therapy. The hypervariable region (HVR) of HCV in the sera of patients was amplified by reverse trancription-polymerase chain reaction (RT-PCR), and the variation of amino acid sequence in this region was determined. Serum alanine aminotransferase (ALT) activities in five patients treated for six months with IF-α fell to the normal range, when HCV was not detected in the sera of three patients. The nucleotide sequence variation in HVR of HCV in the sera of five patients who responded well to the IF-α. therapy was relatively less than that in another five patients who did not respond to IF-α therapy and those in the control patients. These results indicate that the effectiveness of IF-α therapy was related to the sequence variation of HVR of HCV. This may have resulted from the selection pressure by humoral antibodies directed to HVR of HCV. It is concluded that the higher rate of sequence variation in HVR of HCV was compatible with a lower degree of effectiveness of IF-α therapy.
AB - Ten patients with hepatitis C virus (HCV) infection (experimental group) were treated with interferon-α (IF-α). Dosage was six million units per day for one week and then three times a week for another six months. Seven HCV-infected patients (control group) did not receive IF-α therapy. The hypervariable region (HVR) of HCV in the sera of patients was amplified by reverse trancription-polymerase chain reaction (RT-PCR), and the variation of amino acid sequence in this region was determined. Serum alanine aminotransferase (ALT) activities in five patients treated for six months with IF-α fell to the normal range, when HCV was not detected in the sera of three patients. The nucleotide sequence variation in HVR of HCV in the sera of five patients who responded well to the IF-α. therapy was relatively less than that in another five patients who did not respond to IF-α therapy and those in the control patients. These results indicate that the effectiveness of IF-α therapy was related to the sequence variation of HVR of HCV. This may have resulted from the selection pressure by humoral antibodies directed to HVR of HCV. It is concluded that the higher rate of sequence variation in HVR of HCV was compatible with a lower degree of effectiveness of IF-α therapy.
UR - http://www.scopus.com/inward/record.url?scp=0029989728&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0029989728&partnerID=8YFLogxK
U2 - 10.1002/(SICI)1096-9071(199606)49:2<95::AID-JMV5>3.0.CO;2-E
DO - 10.1002/(SICI)1096-9071(199606)49:2<95::AID-JMV5>3.0.CO;2-E
M3 - Article
VL - 49
SP - 95
EP - 102
JO - Journal of Medical Virology
JF - Journal of Medical Virology
SN - 0146-6615
IS - 2
ER -