Nutritional conditions regulate transcriptional activity of SF-1 by controlling sumoylation and ubiquitination

Jiwon Lee; Dong Joo Yang; Syann Lee; Gary D. Hammer; Ki Woo Kim; Joel K. Elmquist

doi:10.1038/srep19143

Nutritional conditions regulate transcriptional activity of SF-1 by controlling sumoylation and ubiquitination

Jiwon Lee, Dong Joo Yang, Syann Lee, Gary D. Hammer, Ki Woo Kim, Joel K. Elmquist

Department of Oral Biology

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

Abstract

Steroidogenic factor 1 (SF-1) is a transcription factor expressed in the ventral medial nucleus of the hypothalamus that regulates energy homeostasis. However, the molecular mechanisms of SF-1 in the control of energy balance are largely unknown. Here, we show that nutritional conditions, such as the presence or absence of serum, affect SF-1 action. Serum starvation significantly decreased hypothalamic SF-1 levels by promoting ubiquitin-dependent degradation, and sumoylation was required for this process. SF-1 transcriptional activity was also differentially regulated by nutritional status. Under normal conditions, the transcriptional activity of hypothalamic SF-1 was activated by SUMO, but this was attenuated during starvation. Taken together, these results indicate that sumoylation and ubiquitination play crucial roles in the regulation of SF-1 function and that these effects are dependent on nutritional conditions, further supporting the importance of SF-1 in the control of energy homeostasis.

Original language	English
Article number	19143
Journal	Scientific reports
Volume	6
DOIs	https://doi.org/10.1038/srep19143
Publication status	Published - 2016 Jan 11

Bibliographical note

Funding Information:
We thank Dr. Hongtao Yu (UT Southwestern Medical Center) for providing GFP-SUMO constructs. This work was supported by NIH grants R01DK088423, R37DK053301 (to J.K.E) and by National Research Foundation NRF-2013R1A1A1007693 and Yonsei University Wonju College of Medicine YUWCM-2013-53 (to K.W.K). J.L. is supported by a Green Center Trainee Fellowship from UT Southwestern Medical Center and D.J.Y is supported by Global Ph.D. Fellowship (NRF-2015H1A2A1032009).

All Science Journal Classification (ASJC) codes

General

Access to Document

10.1038/srep19143

Cite this

@article{bc175d9844c747f8b41c8dbb2654c78b,

title = "Nutritional conditions regulate transcriptional activity of SF-1 by controlling sumoylation and ubiquitination",

abstract = "Steroidogenic factor 1 (SF-1) is a transcription factor expressed in the ventral medial nucleus of the hypothalamus that regulates energy homeostasis. However, the molecular mechanisms of SF-1 in the control of energy balance are largely unknown. Here, we show that nutritional conditions, such as the presence or absence of serum, affect SF-1 action. Serum starvation significantly decreased hypothalamic SF-1 levels by promoting ubiquitin-dependent degradation, and sumoylation was required for this process. SF-1 transcriptional activity was also differentially regulated by nutritional status. Under normal conditions, the transcriptional activity of hypothalamic SF-1 was activated by SUMO, but this was attenuated during starvation. Taken together, these results indicate that sumoylation and ubiquitination play crucial roles in the regulation of SF-1 function and that these effects are dependent on nutritional conditions, further supporting the importance of SF-1 in the control of energy homeostasis.",

author = "Jiwon Lee and Yang, {Dong Joo} and Syann Lee and Hammer, {Gary D.} and Kim, {Ki Woo} and Elmquist, {Joel K.}",

note = "Funding Information: We thank Dr. Hongtao Yu (UT Southwestern Medical Center) for providing GFP-SUMO constructs. This work was supported by NIH grants R01DK088423, R37DK053301 (to J.K.E) and by National Research Foundation NRF-2013R1A1A1007693 and Yonsei University Wonju College of Medicine YUWCM-2013-53 (to K.W.K). J.L. is supported by a Green Center Trainee Fellowship from UT Southwestern Medical Center and D.J.Y is supported by Global Ph.D. Fellowship (NRF-2015H1A2A1032009).",

year = "2016",

month = jan,

day = "11",

doi = "10.1038/srep19143",

language = "English",

volume = "6",

journal = "Scientific Reports",

issn = "2045-2322",

publisher = "Nature Publishing Group",

}

TY - JOUR

T1 - Nutritional conditions regulate transcriptional activity of SF-1 by controlling sumoylation and ubiquitination

AU - Lee, Jiwon

AU - Yang, Dong Joo

AU - Lee, Syann

AU - Hammer, Gary D.

AU - Kim, Ki Woo

AU - Elmquist, Joel K.

N1 - Funding Information: We thank Dr. Hongtao Yu (UT Southwestern Medical Center) for providing GFP-SUMO constructs. This work was supported by NIH grants R01DK088423, R37DK053301 (to J.K.E) and by National Research Foundation NRF-2013R1A1A1007693 and Yonsei University Wonju College of Medicine YUWCM-2013-53 (to K.W.K). J.L. is supported by a Green Center Trainee Fellowship from UT Southwestern Medical Center and D.J.Y is supported by Global Ph.D. Fellowship (NRF-2015H1A2A1032009).

PY - 2016/1/11

Y1 - 2016/1/11

N2 - Steroidogenic factor 1 (SF-1) is a transcription factor expressed in the ventral medial nucleus of the hypothalamus that regulates energy homeostasis. However, the molecular mechanisms of SF-1 in the control of energy balance are largely unknown. Here, we show that nutritional conditions, such as the presence or absence of serum, affect SF-1 action. Serum starvation significantly decreased hypothalamic SF-1 levels by promoting ubiquitin-dependent degradation, and sumoylation was required for this process. SF-1 transcriptional activity was also differentially regulated by nutritional status. Under normal conditions, the transcriptional activity of hypothalamic SF-1 was activated by SUMO, but this was attenuated during starvation. Taken together, these results indicate that sumoylation and ubiquitination play crucial roles in the regulation of SF-1 function and that these effects are dependent on nutritional conditions, further supporting the importance of SF-1 in the control of energy homeostasis.

AB - Steroidogenic factor 1 (SF-1) is a transcription factor expressed in the ventral medial nucleus of the hypothalamus that regulates energy homeostasis. However, the molecular mechanisms of SF-1 in the control of energy balance are largely unknown. Here, we show that nutritional conditions, such as the presence or absence of serum, affect SF-1 action. Serum starvation significantly decreased hypothalamic SF-1 levels by promoting ubiquitin-dependent degradation, and sumoylation was required for this process. SF-1 transcriptional activity was also differentially regulated by nutritional status. Under normal conditions, the transcriptional activity of hypothalamic SF-1 was activated by SUMO, but this was attenuated during starvation. Taken together, these results indicate that sumoylation and ubiquitination play crucial roles in the regulation of SF-1 function and that these effects are dependent on nutritional conditions, further supporting the importance of SF-1 in the control of energy homeostasis.

UR - http://www.scopus.com/inward/record.url?scp=84954100396&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84954100396&partnerID=8YFLogxK

U2 - 10.1038/srep19143

DO - 10.1038/srep19143

M3 - Article

C2 - 26750456

AN - SCOPUS:84954100396

SN - 2045-2322

VL - 6

JO - Scientific Reports

JF - Scientific Reports

M1 - 19143

ER -

Nutritional conditions regulate transcriptional activity of SF-1 by controlling sumoylation and ubiquitination

Abstract

Bibliographical note

All Science Journal Classification (ASJC) codes

Access to Document

Other files and links

Fingerprint

Cite this