Nutritional conditions regulate transcriptional activity of SF-1 by controlling sumoylation and ubiquitination

Jiwon Lee, Dong Joo Yang, Syann Lee, Gary D. Hammer, Kiwoo Kim, Joel K. Elmquist

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Steroidogenic factor 1 (SF-1) is a transcription factor expressed in the ventral medial nucleus of the hypothalamus that regulates energy homeostasis. However, the molecular mechanisms of SF-1 in the control of energy balance are largely unknown. Here, we show that nutritional conditions, such as the presence or absence of serum, affect SF-1 action. Serum starvation significantly decreased hypothalamic SF-1 levels by promoting ubiquitin-dependent degradation, and sumoylation was required for this process. SF-1 transcriptional activity was also differentially regulated by nutritional status. Under normal conditions, the transcriptional activity of hypothalamic SF-1 was activated by SUMO, but this was attenuated during starvation. Taken together, these results indicate that sumoylation and ubiquitination play crucial roles in the regulation of SF-1 function and that these effects are dependent on nutritional conditions, further supporting the importance of SF-1 in the control of energy homeostasis.

Original languageEnglish
Article number19143
JournalScientific reports
Volume6
DOIs
Publication statusPublished - 2016 Jan 11

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Steroidogenic Factor 1
Sumoylation
Ubiquitination
Starvation
Homeostasis
Middle Hypothalamus
Ubiquitin
Nutritional Status
Serum
Transcription Factors

All Science Journal Classification (ASJC) codes

  • General

Cite this

Lee, Jiwon ; Yang, Dong Joo ; Lee, Syann ; Hammer, Gary D. ; Kim, Kiwoo ; Elmquist, Joel K. / Nutritional conditions regulate transcriptional activity of SF-1 by controlling sumoylation and ubiquitination. In: Scientific reports. 2016 ; Vol. 6.
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Nutritional conditions regulate transcriptional activity of SF-1 by controlling sumoylation and ubiquitination. / Lee, Jiwon; Yang, Dong Joo; Lee, Syann; Hammer, Gary D.; Kim, Kiwoo; Elmquist, Joel K.

In: Scientific reports, Vol. 6, 19143, 11.01.2016.

Research output: Contribution to journalArticle

TY - JOUR

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AU - Lee, Jiwon

AU - Yang, Dong Joo

AU - Lee, Syann

AU - Hammer, Gary D.

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AU - Elmquist, Joel K.

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AB - Steroidogenic factor 1 (SF-1) is a transcription factor expressed in the ventral medial nucleus of the hypothalamus that regulates energy homeostasis. However, the molecular mechanisms of SF-1 in the control of energy balance are largely unknown. Here, we show that nutritional conditions, such as the presence or absence of serum, affect SF-1 action. Serum starvation significantly decreased hypothalamic SF-1 levels by promoting ubiquitin-dependent degradation, and sumoylation was required for this process. SF-1 transcriptional activity was also differentially regulated by nutritional status. Under normal conditions, the transcriptional activity of hypothalamic SF-1 was activated by SUMO, but this was attenuated during starvation. Taken together, these results indicate that sumoylation and ubiquitination play crucial roles in the regulation of SF-1 function and that these effects are dependent on nutritional conditions, further supporting the importance of SF-1 in the control of energy homeostasis.

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