O-GlcNAc modification is essential for the regulation of autophagy in Drosophila melanogaster

Sujin Park, Yangsin Lee, Jin Won Pak, Hanbyeol Kim, Hyeonjin Choi, Jae Woo Kim, Jürgen Roth, Jin Won Cho

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

O-GlcNAcylation is a dynamic post-translational modification that takes place on ser/thr residues of nucleocytoplasmic proteins. O-GlcNAcylation regulates almost all cellular events as a nutrient sensor, a transcriptional and translational regulator, and a disease-related factor. Although the role of O-GlcNAcylation in insulin signaling and metabolism are well established, the relationship between O-GlcNAcylation and autophagy is largely unknown. Here, we manipulated O-GlcNAcylation in Drosophila and found that it regulates autophagy through Akt/dFOXO signaling. We demonstrate that O-GlcNAcylation and the levels of O-GlcNAc transferase (OGT) are increased during starvation. Furthermore, Atg proteins and autolysosomes are increased in OGT-reduced flies without fasting. Atg proteins and autophagosomes are reduced in OGT-overexpressing flies. Our results suggest that not only autophagy gene expression but also autophagic structures are regulated by OGT through Akt and dFOXO. These data imply that O-GlcNAcylation is important in modulating autophagy as well as insulin signaling in Drosophila.

Original languageEnglish
Pages (from-to)3173-3183
Number of pages11
JournalCellular and Molecular Life Sciences
Volume72
Issue number16
DOIs
Publication statusPublished - 2015 Aug 25

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Autophagy
Drosophila melanogaster
Diptera
Drosophila
Insulin
Proteins
Post Translational Protein Processing
Starvation
Fasting
Gene Expression
Food
O-GlcNAc transferase

All Science Journal Classification (ASJC) codes

  • Molecular Medicine
  • Molecular Biology
  • Pharmacology
  • Cellular and Molecular Neuroscience
  • Cell Biology

Cite this

Park, Sujin ; Lee, Yangsin ; Pak, Jin Won ; Kim, Hanbyeol ; Choi, Hyeonjin ; Kim, Jae Woo ; Roth, Jürgen ; Cho, Jin Won. / O-GlcNAc modification is essential for the regulation of autophagy in Drosophila melanogaster. In: Cellular and Molecular Life Sciences. 2015 ; Vol. 72, No. 16. pp. 3173-3183.
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O-GlcNAc modification is essential for the regulation of autophagy in Drosophila melanogaster. / Park, Sujin; Lee, Yangsin; Pak, Jin Won; Kim, Hanbyeol; Choi, Hyeonjin; Kim, Jae Woo; Roth, Jürgen; Cho, Jin Won.

In: Cellular and Molecular Life Sciences, Vol. 72, No. 16, 25.08.2015, p. 3173-3183.

Research output: Contribution to journalArticle

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AU - Kim, Jae Woo

AU - Roth, Jürgen

AU - Cho, Jin Won

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