Abstract
O-GlcNAcylation is a dynamic post-translational modification that takes place on ser/thr residues of nucleocytoplasmic proteins. O-GlcNAcylation regulates almost all cellular events as a nutrient sensor, a transcriptional and translational regulator, and a disease-related factor. Although the role of O-GlcNAcylation in insulin signaling and metabolism are well established, the relationship between O-GlcNAcylation and autophagy is largely unknown. Here, we manipulated O-GlcNAcylation in Drosophila and found that it regulates autophagy through Akt/dFOXO signaling. We demonstrate that O-GlcNAcylation and the levels of O-GlcNAc transferase (OGT) are increased during starvation. Furthermore, Atg proteins and autolysosomes are increased in OGT-reduced flies without fasting. Atg proteins and autophagosomes are reduced in OGT-overexpressing flies. Our results suggest that not only autophagy gene expression but also autophagic structures are regulated by OGT through Akt and dFOXO. These data imply that O-GlcNAcylation is important in modulating autophagy as well as insulin signaling in Drosophila.
| Original language | English |
|---|---|
| Pages (from-to) | 3173-3183 |
| Number of pages | 11 |
| Journal | Cellular and Molecular Life Sciences |
| Volume | 72 |
| Issue number | 16 |
| DOIs | |
| Publication status | Published - 2015 Aug 25 |
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All Science Journal Classification (ASJC) codes
- Molecular Medicine
- Molecular Biology
- Pharmacology
- Cellular and Molecular Neuroscience
- Cell Biology
Cite this
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O-GlcNAc modification is essential for the regulation of autophagy in Drosophila melanogaster. / Park, Sujin; Lee, Yangsin; Pak, Jin Won; Kim, Hanbyeol; Choi, Hyeonjin; Kim, Jae Woo; Roth, Jürgen; Cho, Jin Won.
In: Cellular and Molecular Life Sciences, Vol. 72, No. 16, 25.08.2015, p. 3173-3183.Research output: Contribution to journal › Article
TY - JOUR
T1 - O-GlcNAc modification is essential for the regulation of autophagy in Drosophila melanogaster
AU - Park, Sujin
AU - Lee, Yangsin
AU - Pak, Jin Won
AU - Kim, Hanbyeol
AU - Choi, Hyeonjin
AU - Kim, Jae Woo
AU - Roth, Jürgen
AU - Cho, Jin Won
PY - 2015/8/25
Y1 - 2015/8/25
N2 - O-GlcNAcylation is a dynamic post-translational modification that takes place on ser/thr residues of nucleocytoplasmic proteins. O-GlcNAcylation regulates almost all cellular events as a nutrient sensor, a transcriptional and translational regulator, and a disease-related factor. Although the role of O-GlcNAcylation in insulin signaling and metabolism are well established, the relationship between O-GlcNAcylation and autophagy is largely unknown. Here, we manipulated O-GlcNAcylation in Drosophila and found that it regulates autophagy through Akt/dFOXO signaling. We demonstrate that O-GlcNAcylation and the levels of O-GlcNAc transferase (OGT) are increased during starvation. Furthermore, Atg proteins and autolysosomes are increased in OGT-reduced flies without fasting. Atg proteins and autophagosomes are reduced in OGT-overexpressing flies. Our results suggest that not only autophagy gene expression but also autophagic structures are regulated by OGT through Akt and dFOXO. These data imply that O-GlcNAcylation is important in modulating autophagy as well as insulin signaling in Drosophila.
AB - O-GlcNAcylation is a dynamic post-translational modification that takes place on ser/thr residues of nucleocytoplasmic proteins. O-GlcNAcylation regulates almost all cellular events as a nutrient sensor, a transcriptional and translational regulator, and a disease-related factor. Although the role of O-GlcNAcylation in insulin signaling and metabolism are well established, the relationship between O-GlcNAcylation and autophagy is largely unknown. Here, we manipulated O-GlcNAcylation in Drosophila and found that it regulates autophagy through Akt/dFOXO signaling. We demonstrate that O-GlcNAcylation and the levels of O-GlcNAc transferase (OGT) are increased during starvation. Furthermore, Atg proteins and autolysosomes are increased in OGT-reduced flies without fasting. Atg proteins and autophagosomes are reduced in OGT-overexpressing flies. Our results suggest that not only autophagy gene expression but also autophagic structures are regulated by OGT through Akt and dFOXO. These data imply that O-GlcNAcylation is important in modulating autophagy as well as insulin signaling in Drosophila.
UR - http://www.scopus.com/inward/record.url?scp=84937739068&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84937739068&partnerID=8YFLogxK
U2 - 10.1007/s00018-015-1889-z
DO - 10.1007/s00018-015-1889-z
M3 - Article
C2 - 25840568
AN - SCOPUS:84937739068
VL - 72
SP - 3173
EP - 3183
JO - Cellular and Molecular Life Sciences
JF - Cellular and Molecular Life Sciences
SN - 1420-682X
IS - 16
ER -