O-GlcNAcylation of tubulin inhibits its polymerization

Suena Ji, Jeong Gu Kang, Sang Yoon Park, Joohun Lee, Young J. Oh, Jin Won Cho

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

The attachment of O-linked β-N-acetylglucosamine (O-GlcNAc) to proteins is an abundant and reversible modification that involves many cellular processes including transcription, translation, cell proliferation, apoptosis, and signal transduction. Here, we found that the O-GlcNAc modification pattern was altered during all-trans retinoic acid (tRA)-induced neurite outgrowth in the MN9D neuronal cell line. We identified several O-GlcNAcylated proteins using mass spectrometric analysis, including α- and β-tubulin. Further analysis of α- and β-tubulin revealed that O-GlcNAcylated peptides mapped between residues 173 and 185 of α-tubulin and between residues 216 and 238 of β-tubulin, respectively. We found that an increase in α-tubulin O-GlcNAcylation reduced heterodimerization and that O-GlcNAcylated tubulin did not polymerize into microtubules. Consequently, when O-GlcNAcase inhibitors were co-incubated with tRA, the extent of neurite outgrowth was decreased by 20% compared to control. Thus, our data indicate that the O-GlcNAcylation of tubulin negatively regulates microtubule formation.

Original languageEnglish
Pages (from-to)809-818
Number of pages10
JournalAmino Acids
Volume40
Issue number3
DOIs
Publication statusPublished - 2011 Mar 1

Fingerprint

Tubulin
Polymerization
Tretinoin
Microtubules
Signal transduction
Acetylglucosamine
Cell proliferation
Transcription
Signal Transduction
Proteins
Cells
Cell Proliferation
Apoptosis
Cell Line
Peptides

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Clinical Biochemistry
  • Organic Chemistry

Cite this

Ji, Suena ; Kang, Jeong Gu ; Park, Sang Yoon ; Lee, Joohun ; Oh, Young J. ; Cho, Jin Won. / O-GlcNAcylation of tubulin inhibits its polymerization. In: Amino Acids. 2011 ; Vol. 40, No. 3. pp. 809-818.
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O-GlcNAcylation of tubulin inhibits its polymerization. / Ji, Suena; Kang, Jeong Gu; Park, Sang Yoon; Lee, Joohun; Oh, Young J.; Cho, Jin Won.

In: Amino Acids, Vol. 40, No. 3, 01.03.2011, p. 809-818.

Research output: Contribution to journalArticle

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