Optical-resolution photoacoustic microscopy (OR-PAM) becomes a premier microscopic imaging tool in biomedicine because it provides agent-free optical absorption information in tissues. By tightly focusing light to a spot, a significantly improved lateral resolution can be achieved in OR-PAM. The focal spot size is typically determined by the numerical aperture of the used objective lens. Here, we demonstrate objective-free OR-PAM using a fiber optic Bessel beam generator. In this approach, no objective lens is required and, beneficially, the complexities of conventional OR-PAM systems can be greatly relieved. We have obtained photoacoustic images of a carbon fiber with a diameter of ≪6 μm, whose lateral resolution was measured to be better than 6 to 7 μm.
Bibliographical noteFunding Information:
This research was supported by the University at Buffalo Research Foundation faculty start-up fund, the Pilot Studies program of the University at Buffalo Clinical and Translational Research Center and the Buffalo Translational Consortium to C. K., supported by the Brain Korea 21 Project, the Korea NRF, and the Korea MEST-2011-00181613 to K.O., and supported by Ministry of Health & Welfare through the Korea Healthcare technology R&D Project A102024-1011-0000200, the Korea NRF, and the Korea MEST -2012-0005047 to J. K.
All Science Journal Classification (ASJC) codes
- Electronic, Optical and Magnetic Materials
- Atomic and Molecular Physics, and Optics
- Biomedical Engineering