An effective volumetric measurement method for determination of the 3D position of a single particle based on off-Axis digital holographic microscopy is presented in this paper. 3 μm polystyrene bead suspended water is used as a particle to be traced in our experiment. We have demonstrated the feasibility of our method by observing the bead undergoing Brownian motion in water by implemented setup. For fast determination, a series of transverse intensity images are numerically reconstructed from a single hologram of the bead, then centroid technique is applied to the reconstructed images for enhancing the position resolution. Nano-meter scale resolution with a frame rate of 30 is achieved in this experiment in both lateral and transverse direction.
|Title of host publication||International Conference on Nano-Bio Sensing, Imaging, and Spectroscopy 2015, NBSIS 2015|
|Editors||Donghyun Kim, Min-Gon Kim, Seung-Han Park|
|Publication status||Published - 2015|
|Event||2nd International Conference on Nano-Bio Sensing, Imaging, and Spectroscopy, NBSIS 2015 - Jeju, Korea, Republic of|
Duration: 2015 Feb 25 → 2015 Feb 27
|Name||Progress in Biomedical Optics and Imaging - Proceedings of SPIE|
|Other||2nd International Conference on Nano-Bio Sensing, Imaging, and Spectroscopy, NBSIS 2015|
|Country||Korea, Republic of|
|Period||15/2/25 → 15/2/27|
Bibliographical notePublisher Copyright:
© 2015 SPIE.
All Science Journal Classification (ASJC) codes
- Electronic, Optical and Magnetic Materials
- Atomic and Molecular Physics, and Optics
- Radiology Nuclear Medicine and imaging