Abstract
An effective volumetric measurement method for determination of the 3D position of a single particle based on off-Axis digital holographic microscopy is presented in this paper. 3 μm polystyrene bead suspended water is used as a particle to be traced in our experiment. We have demonstrated the feasibility of our method by observing the bead undergoing Brownian motion in water by implemented setup. For fast determination, a series of transverse intensity images are numerically reconstructed from a single hologram of the bead, then centroid technique is applied to the reconstructed images for enhancing the position resolution. Nano-meter scale resolution with a frame rate of 30 is achieved in this experiment in both lateral and transverse direction.
| Original language | English |
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| Title of host publication | International Conference on Nano-Bio Sensing, Imaging, and Spectroscopy 2015, NBSIS 2015 |
| Editors | Donghyun Kim, Min-Gon Kim, Seung-Han Park |
| Publisher | SPIE |
| ISBN (Electronic) | 9781628416794 |
| DOIs | |
| Publication status | Published - 2015 |
| Event | 2nd International Conference on Nano-Bio Sensing, Imaging, and Spectroscopy, NBSIS 2015 - Jeju, Korea, Republic of Duration: 2015 Feb 25 → 2015 Feb 27 |
Publication series
| Name | Progress in Biomedical Optics and Imaging - Proceedings of SPIE |
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| Volume | 9523 |
| ISSN (Print) | 1605-7422 |
Other
| Other | 2nd International Conference on Nano-Bio Sensing, Imaging, and Spectroscopy, NBSIS 2015 |
|---|---|
| Country/Territory | Korea, Republic of |
| City | Jeju |
| Period | 15/2/25 → 15/2/27 |
Bibliographical note
Publisher Copyright:© 2015 SPIE.
All Science Journal Classification (ASJC) codes
- Electronic, Optical and Magnetic Materials
- Biomaterials
- Atomic and Molecular Physics, and Optics
- Radiology Nuclear Medicine and imaging