Observation of Brownian motion of a micrometer-size bead in water by off-Axis digital holographic microscopy

Hee Jung Lee, Yoon Sung Bae, Phil Jun Jeon, Dug Young Kim

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

An effective volumetric measurement method for determination of the 3D position of a single particle based on off-Axis digital holographic microscopy is presented in this paper. 3 μm polystyrene bead suspended water is used as a particle to be traced in our experiment. We have demonstrated the feasibility of our method by observing the bead undergoing Brownian motion in water by implemented setup. For fast determination, a series of transverse intensity images are numerically reconstructed from a single hologram of the bead, then centroid technique is applied to the reconstructed images for enhancing the position resolution. Nano-meter scale resolution with a frame rate of 30 is achieved in this experiment in both lateral and transverse direction.

Original languageEnglish
Title of host publicationInternational Conference on Nano-Bio Sensing, Imaging, and Spectroscopy 2015, NBSIS 2015
EditorsDonghyun Kim, Min-Gon Kim, Seung-Han Park
PublisherSPIE
ISBN (Electronic)9781628416794
DOIs
Publication statusPublished - 2015
Event2nd International Conference on Nano-Bio Sensing, Imaging, and Spectroscopy, NBSIS 2015 - Jeju, Korea, Republic of
Duration: 2015 Feb 252015 Feb 27

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume9523
ISSN (Print)1605-7422

Other

Other2nd International Conference on Nano-Bio Sensing, Imaging, and Spectroscopy, NBSIS 2015
CountryKorea, Republic of
CityJeju
Period15/2/2515/2/27

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

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  • Cite this

    Lee, H. J., Bae, Y. S., Jeon, P. J., & Kim, D. Y. (2015). Observation of Brownian motion of a micrometer-size bead in water by off-Axis digital holographic microscopy. In D. Kim, M-G. Kim, & S-H. Park (Eds.), International Conference on Nano-Bio Sensing, Imaging, and Spectroscopy 2015, NBSIS 2015 [95230A] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 9523). SPIE. https://doi.org/10.1117/12.2189433