We have developed a high-speed and high-resolution multimodal nonlinear optical microscope, which can acquire in vivo label and label-free 3D images. Using this microscope, we could successfully observe dynamic images of the zebrafish heartbeat with 33-millisecond intervals without staining process, indicating 1.68 beats per second. In addition, we show that the label-free, high-speed, real-time images can be obtained during somatic cell divisions in the zebrafish’s embryo.
Bibliographical noteFunding Information:
This research was supported by the BK21 PLUS program and the Pioneer Research Center Program (20100002212) through the National Research Foundation (NRF) funded by the Ministry of Education of Korea.
© 2019, The Korean Physical Society.
All Science Journal Classification (ASJC) codes
- Physics and Astronomy(all)