Occurrence of extended-spectrum β-lactamases among chromosomal AmpC-producing Enterobacter cloacae, Citrobacter freundii, and Serratia marcescens in Korea and investigation of screening criteria

Yeon Joon Park; Sun Young Park; Eun Jee Oh; Jung Jun Park; Kyo Young Lee; Gun Jo Woo; Kyungwon Lee

doi:10.1016/j.diagmicrobio.2004.11.009

Occurrence of extended-spectrum β-lactamases among chromosomal AmpC-producing Enterobacter cloacae, Citrobacter freundii, and Serratia marcescens in Korea and investigation of screening criteria

Yeon Joon Park, Sun Young Park, Eun Jee Oh, Jung Jun Park, Kyo Young Lee, Gun Jo Woo, Kyungwon Lee

Department of Laboratory Medicine

Research output: Contribution to journal › Article › peer-review

57 Citations (Scopus)

Abstract

We assessed the occurrence and screening criterion for extended-spectrum β-lactamases (ESBLs) among AmpC-producing Enterobacter cloacae, Citrobacter freundii, and Serratia marcescens. The 413 isolates (158 E. cloacae, 126 C. freundii, and 129 S. marcescens) isolated from 11 clinical laboratories in Korea were investigated. ESBL production was confirmed by double-disk synergy test and inhibitor-potentiated diffusion test using ceftazidime (CAZ), cefotaxime (CTX), aztreonam (AZT), and cefepime (FEP) with or without clavulanic acid. One hundred seven isolates (25.9%) were as ESBL producers. Of them, resistance was transferred by conjugation in 82 isolates. In transconjugants, structural genes for CTX-M (53.7%), TEM (46.3%), SHV (29.3%) were found. To evaluate the ESBL screening minimum inhibitory concentration (MIC) criteria, MICs for cefuroxime, CAZ, CTX, AZT, and FEP were determined and cutoff value was selected using receiver operator characteristic curve. The FEP MIC ≥ 1 μg/mL had the highest sensitivity (95.3%), specificity (82.7%), and positive (65.8%) and negative predictive values (98.3%).

Original language	English
Pages (from-to)	265-269
Number of pages	5
Journal	Diagnostic Microbiology and Infectious Disease
Volume	51
Issue number	4
DOIs	https://doi.org/10.1016/j.diagmicrobio.2004.11.009
Publication status	Published - 2005 Apr

Bibliographical note

Funding Information:
The authors thank all the contributing laboratories that provided isolates for this study. This work was supported by grant from Korea Food and Drug Administration in 2003.

All Science Journal Classification (ASJC) codes

Microbiology (medical)
Infectious Diseases

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.diagmicrobio.2004.11.009

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Park, Y. J., Park, S. Y., Oh, E. J., Park, J. J., Lee, K. Y., Woo, G. J., & Lee, K. (2005). Occurrence of extended-spectrum β-lactamases among chromosomal AmpC-producing Enterobacter cloacae, Citrobacter freundii, and Serratia marcescens in Korea and investigation of screening criteria. Diagnostic Microbiology and Infectious Disease, 51(4), 265-269. https://doi.org/10.1016/j.diagmicrobio.2004.11.009

@article{ea4b6c20208e44ecaa999ce0a620da37,

title = "Occurrence of extended-spectrum β-lactamases among chromosomal AmpC-producing Enterobacter cloacae, Citrobacter freundii, and Serratia marcescens in Korea and investigation of screening criteria",

abstract = "We assessed the occurrence and screening criterion for extended-spectrum β-lactamases (ESBLs) among AmpC-producing Enterobacter cloacae, Citrobacter freundii, and Serratia marcescens. The 413 isolates (158 E. cloacae, 126 C. freundii, and 129 S. marcescens) isolated from 11 clinical laboratories in Korea were investigated. ESBL production was confirmed by double-disk synergy test and inhibitor-potentiated diffusion test using ceftazidime (CAZ), cefotaxime (CTX), aztreonam (AZT), and cefepime (FEP) with or without clavulanic acid. One hundred seven isolates (25.9%) were as ESBL producers. Of them, resistance was transferred by conjugation in 82 isolates. In transconjugants, structural genes for CTX-M (53.7%), TEM (46.3%), SHV (29.3%) were found. To evaluate the ESBL screening minimum inhibitory concentration (MIC) criteria, MICs for cefuroxime, CAZ, CTX, AZT, and FEP were determined and cutoff value was selected using receiver operator characteristic curve. The FEP MIC ≥ 1 μg/mL had the highest sensitivity (95.3%), specificity (82.7%), and positive (65.8%) and negative predictive values (98.3%).",

author = "Park, {Yeon Joon} and Park, {Sun Young} and Oh, {Eun Jee} and Park, {Jung Jun} and Lee, {Kyo Young} and Woo, {Gun Jo} and Kyungwon Lee",

note = "Funding Information: The authors thank all the contributing laboratories that provided isolates for this study. This work was supported by grant from Korea Food and Drug Administration in 2003.",

year = "2005",

month = apr,

doi = "10.1016/j.diagmicrobio.2004.11.009",

language = "English",

volume = "51",

pages = "265--269",

journal = "Diagnostic Microbiology and Infectious Disease",

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Park, YJ, Park, SY, Oh, EJ, Park, JJ, Lee, KY, Woo, GJ & Lee, K 2005, 'Occurrence of extended-spectrum β-lactamases among chromosomal AmpC-producing Enterobacter cloacae, Citrobacter freundii, and Serratia marcescens in Korea and investigation of screening criteria', Diagnostic Microbiology and Infectious Disease, vol. 51, no. 4, pp. 265-269. https://doi.org/10.1016/j.diagmicrobio.2004.11.009

Occurrence of extended-spectrum β-lactamases among chromosomal AmpC-producing Enterobacter cloacae, Citrobacter freundii, and Serratia marcescens in Korea and investigation of screening criteria. / Park, Yeon Joon; Park, Sun Young; Oh, Eun Jee et al.

In: Diagnostic Microbiology and Infectious Disease, Vol. 51, No. 4, 04.2005, p. 265-269.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Occurrence of extended-spectrum β-lactamases among chromosomal AmpC-producing Enterobacter cloacae, Citrobacter freundii, and Serratia marcescens in Korea and investigation of screening criteria

AU - Park, Yeon Joon

AU - Park, Sun Young

AU - Oh, Eun Jee

AU - Park, Jung Jun

AU - Lee, Kyo Young

AU - Woo, Gun Jo

AU - Lee, Kyungwon

N1 - Funding Information: The authors thank all the contributing laboratories that provided isolates for this study. This work was supported by grant from Korea Food and Drug Administration in 2003.

PY - 2005/4

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N2 - We assessed the occurrence and screening criterion for extended-spectrum β-lactamases (ESBLs) among AmpC-producing Enterobacter cloacae, Citrobacter freundii, and Serratia marcescens. The 413 isolates (158 E. cloacae, 126 C. freundii, and 129 S. marcescens) isolated from 11 clinical laboratories in Korea were investigated. ESBL production was confirmed by double-disk synergy test and inhibitor-potentiated diffusion test using ceftazidime (CAZ), cefotaxime (CTX), aztreonam (AZT), and cefepime (FEP) with or without clavulanic acid. One hundred seven isolates (25.9%) were as ESBL producers. Of them, resistance was transferred by conjugation in 82 isolates. In transconjugants, structural genes for CTX-M (53.7%), TEM (46.3%), SHV (29.3%) were found. To evaluate the ESBL screening minimum inhibitory concentration (MIC) criteria, MICs for cefuroxime, CAZ, CTX, AZT, and FEP were determined and cutoff value was selected using receiver operator characteristic curve. The FEP MIC ≥ 1 μg/mL had the highest sensitivity (95.3%), specificity (82.7%), and positive (65.8%) and negative predictive values (98.3%).

AB - We assessed the occurrence and screening criterion for extended-spectrum β-lactamases (ESBLs) among AmpC-producing Enterobacter cloacae, Citrobacter freundii, and Serratia marcescens. The 413 isolates (158 E. cloacae, 126 C. freundii, and 129 S. marcescens) isolated from 11 clinical laboratories in Korea were investigated. ESBL production was confirmed by double-disk synergy test and inhibitor-potentiated diffusion test using ceftazidime (CAZ), cefotaxime (CTX), aztreonam (AZT), and cefepime (FEP) with or without clavulanic acid. One hundred seven isolates (25.9%) were as ESBL producers. Of them, resistance was transferred by conjugation in 82 isolates. In transconjugants, structural genes for CTX-M (53.7%), TEM (46.3%), SHV (29.3%) were found. To evaluate the ESBL screening minimum inhibitory concentration (MIC) criteria, MICs for cefuroxime, CAZ, CTX, AZT, and FEP were determined and cutoff value was selected using receiver operator characteristic curve. The FEP MIC ≥ 1 μg/mL had the highest sensitivity (95.3%), specificity (82.7%), and positive (65.8%) and negative predictive values (98.3%).

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ER -

Occurrence of extended-spectrum β-lactamases among chromosomal AmpC-producing Enterobacter cloacae, Citrobacter freundii, and Serratia marcescens in Korea and investigation of screening criteria

Abstract

Bibliographical note

All Science Journal Classification (ASJC) codes

UN SDGs

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