Ochrobactrum daejeonense sp. nov., a nitrate-reducing bacterium isolated from sludge of a leachate treatment plant

Sung Geun Woo, Leonid N. Ten, Joonhong Park, Myungjin Lee

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

A Gram-reaction-negative, non-spore-forming, rod-shaped, aerobic bacterial strain, designated MJ11 T, was isolated from sludge of a leachate treatment plant in Daejeon, South Korea, and was characterized taxonomically by using a polyphasic approach. Comparative 16S rRNA gene sequence analysis showed that strain MJ11 T belonged to the family Brucellaceae, class Alphaproteobacteria, and was most closely related to Ochrobactrum ciceri Ca-34 T (97.9% sequence similarity) and Ochrobactrum pituitosum CCUG 50899 T (96.4%). Comparative sequence analyses of the additional phylogenetic marker genes dnaK, groEL and gyrB confirmed the affiliation of strain MJ11 T to the genus Ochrobactrum. The G+C content of the genomic DNA of strain MJ11 T was 59.3 mol%. The detection of a quinone system with ubiquinone Q-10 as the predominant respiratory lipoquinone, a fatty acid profile with C 18:1ω7c (62.6%) and C 19:0 cyclo ω8c (14.2%) as the major components, a polar lipid profile with phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, phosphatidylmonomethylethanolamine, diphosphatidylglycerol and unknown aminolipids AL1 and AL2 as major polar lipids and spermidine and putrescine as the predominant polyamines also supported the affiliation of strain MJ11 T to the genus Ochrobactrum. The DNA-DNA relatedness between strain MJ11 T and Ochrobactrum ciceri DSM 22292 T was 29±7%, clearly showing that the isolate constitutes a new genospecies. Strain MJ11T could be clearly differentiated from its closest neighbours on the basis of its phenotypic, genotypic and chemotaxonomic features. Therefore, strain MJ11 T represents a novel species of the genus Ochrobactrum, for which the name Ochrobactrum daejeonense sp. nov. is proposed. The type strain is MJ11 T (5KCTC 22458 T 5JCM 16234 T).

Original languageEnglish
Pages (from-to)2690-2696
Number of pages7
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume61
Issue number11
DOIs
Publication statusPublished - 2011 Nov 1

Fingerprint

Ochrobactrum
leachates
Sewage
sludge
Nitrates
leachate
nitrates
nitrate
Bacteria
DNA
bacterium
new species
bacteria
lipid
gene
relatedness
Brucellaceae
genomics
fatty acid
Sequence Analysis

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics

Cite this

@article{3b21548d36c149a0b9d1c93dc9c759e7,
title = "Ochrobactrum daejeonense sp. nov., a nitrate-reducing bacterium isolated from sludge of a leachate treatment plant",
abstract = "A Gram-reaction-negative, non-spore-forming, rod-shaped, aerobic bacterial strain, designated MJ11 T, was isolated from sludge of a leachate treatment plant in Daejeon, South Korea, and was characterized taxonomically by using a polyphasic approach. Comparative 16S rRNA gene sequence analysis showed that strain MJ11 T belonged to the family Brucellaceae, class Alphaproteobacteria, and was most closely related to Ochrobactrum ciceri Ca-34 T (97.9{\%} sequence similarity) and Ochrobactrum pituitosum CCUG 50899 T (96.4{\%}). Comparative sequence analyses of the additional phylogenetic marker genes dnaK, groEL and gyrB confirmed the affiliation of strain MJ11 T to the genus Ochrobactrum. The G+C content of the genomic DNA of strain MJ11 T was 59.3 mol{\%}. The detection of a quinone system with ubiquinone Q-10 as the predominant respiratory lipoquinone, a fatty acid profile with C 18:1ω7c (62.6{\%}) and C 19:0 cyclo ω8c (14.2{\%}) as the major components, a polar lipid profile with phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, phosphatidylmonomethylethanolamine, diphosphatidylglycerol and unknown aminolipids AL1 and AL2 as major polar lipids and spermidine and putrescine as the predominant polyamines also supported the affiliation of strain MJ11 T to the genus Ochrobactrum. The DNA-DNA relatedness between strain MJ11 T and Ochrobactrum ciceri DSM 22292 T was 29±7{\%}, clearly showing that the isolate constitutes a new genospecies. Strain MJ11T could be clearly differentiated from its closest neighbours on the basis of its phenotypic, genotypic and chemotaxonomic features. Therefore, strain MJ11 T represents a novel species of the genus Ochrobactrum, for which the name Ochrobactrum daejeonense sp. nov. is proposed. The type strain is MJ11 T (5KCTC 22458 T 5JCM 16234 T).",
author = "Woo, {Sung Geun} and Ten, {Leonid N.} and Joonhong Park and Myungjin Lee",
year = "2011",
month = "11",
day = "1",
doi = "10.1099/ijs.0.025510-0",
language = "English",
volume = "61",
pages = "2690--2696",
journal = "International Journal of Systematic and Evolutionary Microbiology",
issn = "1466-5026",
publisher = "Society for General Microbiology",
number = "11",

}

Ochrobactrum daejeonense sp. nov., a nitrate-reducing bacterium isolated from sludge of a leachate treatment plant. / Woo, Sung Geun; Ten, Leonid N.; Park, Joonhong; Lee, Myungjin.

In: International Journal of Systematic and Evolutionary Microbiology, Vol. 61, No. 11, 01.11.2011, p. 2690-2696.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Ochrobactrum daejeonense sp. nov., a nitrate-reducing bacterium isolated from sludge of a leachate treatment plant

AU - Woo, Sung Geun

AU - Ten, Leonid N.

AU - Park, Joonhong

AU - Lee, Myungjin

PY - 2011/11/1

Y1 - 2011/11/1

N2 - A Gram-reaction-negative, non-spore-forming, rod-shaped, aerobic bacterial strain, designated MJ11 T, was isolated from sludge of a leachate treatment plant in Daejeon, South Korea, and was characterized taxonomically by using a polyphasic approach. Comparative 16S rRNA gene sequence analysis showed that strain MJ11 T belonged to the family Brucellaceae, class Alphaproteobacteria, and was most closely related to Ochrobactrum ciceri Ca-34 T (97.9% sequence similarity) and Ochrobactrum pituitosum CCUG 50899 T (96.4%). Comparative sequence analyses of the additional phylogenetic marker genes dnaK, groEL and gyrB confirmed the affiliation of strain MJ11 T to the genus Ochrobactrum. The G+C content of the genomic DNA of strain MJ11 T was 59.3 mol%. The detection of a quinone system with ubiquinone Q-10 as the predominant respiratory lipoquinone, a fatty acid profile with C 18:1ω7c (62.6%) and C 19:0 cyclo ω8c (14.2%) as the major components, a polar lipid profile with phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, phosphatidylmonomethylethanolamine, diphosphatidylglycerol and unknown aminolipids AL1 and AL2 as major polar lipids and spermidine and putrescine as the predominant polyamines also supported the affiliation of strain MJ11 T to the genus Ochrobactrum. The DNA-DNA relatedness between strain MJ11 T and Ochrobactrum ciceri DSM 22292 T was 29±7%, clearly showing that the isolate constitutes a new genospecies. Strain MJ11T could be clearly differentiated from its closest neighbours on the basis of its phenotypic, genotypic and chemotaxonomic features. Therefore, strain MJ11 T represents a novel species of the genus Ochrobactrum, for which the name Ochrobactrum daejeonense sp. nov. is proposed. The type strain is MJ11 T (5KCTC 22458 T 5JCM 16234 T).

AB - A Gram-reaction-negative, non-spore-forming, rod-shaped, aerobic bacterial strain, designated MJ11 T, was isolated from sludge of a leachate treatment plant in Daejeon, South Korea, and was characterized taxonomically by using a polyphasic approach. Comparative 16S rRNA gene sequence analysis showed that strain MJ11 T belonged to the family Brucellaceae, class Alphaproteobacteria, and was most closely related to Ochrobactrum ciceri Ca-34 T (97.9% sequence similarity) and Ochrobactrum pituitosum CCUG 50899 T (96.4%). Comparative sequence analyses of the additional phylogenetic marker genes dnaK, groEL and gyrB confirmed the affiliation of strain MJ11 T to the genus Ochrobactrum. The G+C content of the genomic DNA of strain MJ11 T was 59.3 mol%. The detection of a quinone system with ubiquinone Q-10 as the predominant respiratory lipoquinone, a fatty acid profile with C 18:1ω7c (62.6%) and C 19:0 cyclo ω8c (14.2%) as the major components, a polar lipid profile with phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, phosphatidylmonomethylethanolamine, diphosphatidylglycerol and unknown aminolipids AL1 and AL2 as major polar lipids and spermidine and putrescine as the predominant polyamines also supported the affiliation of strain MJ11 T to the genus Ochrobactrum. The DNA-DNA relatedness between strain MJ11 T and Ochrobactrum ciceri DSM 22292 T was 29±7%, clearly showing that the isolate constitutes a new genospecies. Strain MJ11T could be clearly differentiated from its closest neighbours on the basis of its phenotypic, genotypic and chemotaxonomic features. Therefore, strain MJ11 T represents a novel species of the genus Ochrobactrum, for which the name Ochrobactrum daejeonense sp. nov. is proposed. The type strain is MJ11 T (5KCTC 22458 T 5JCM 16234 T).

UR - http://www.scopus.com/inward/record.url?scp=80355144785&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=80355144785&partnerID=8YFLogxK

U2 - 10.1099/ijs.0.025510-0

DO - 10.1099/ijs.0.025510-0

M3 - Article

C2 - 21169456

AN - SCOPUS:80355144785

VL - 61

SP - 2690

EP - 2696

JO - International Journal of Systematic and Evolutionary Microbiology

JF - International Journal of Systematic and Evolutionary Microbiology

SN - 1466-5026

IS - 11

ER -