Odontoblast TRP channels and thermo/mechanical transmission

A. R. Son, Y. M. Yang, J. H. Hong, S. I. Lee, Y. Shibukawa, D. M. Shin

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Abstract

Odontoblasts function as mechanosensory receptors because of the expression of mechanosensitive channels in these cells. However, it is unclear if odontoblasts direct the signal transmission evoked by heat/cold or osmotic changes. This study investigated the effects of heat/cold or osmotic changes on calcium signaling and the functional expression of the thermo/mechanosensitive transient receptor potential (TRP) channels in primary cultured mouse odontoblastic cells, with the use of RT-PCR, fluorometric calcium imaging, and electrophysiology. TRPV1, TRPV2, TRPV3, TRPV4, and TRPM3 mRNA was expressed, but TRPM8 and TRPA1 mRNA was not. The receptor-specific stimulation of TRPV1-3 (heat-sensing receptors) and TRPV4/ TRPM3 (mechanic receptors) caused increases in the intracellular calcium concentration. Moreover, the channel activities of TRPV1-4 and TRPM3 were confirmed by a whole-cell patch-clamp technique. These results suggest that primary cultured mouse odontoblasts express heat/mechanosensitive TRP channels and play a role in the underlying mechanisms of thermo/mechanosensitive sensory transmission.

Original languageEnglish
Pages (from-to)1014-1019
Number of pages6
JournalJournal of Dental Research
Volume88
Issue number11
DOIs
Publication statusPublished - 2009 Nov 1

All Science Journal Classification (ASJC) codes

  • Dentistry(all)

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    Son, A. R., Yang, Y. M., Hong, J. H., Lee, S. I., Shibukawa, Y., & Shin, D. M. (2009). Odontoblast TRP channels and thermo/mechanical transmission. Journal of Dental Research, 88(11), 1014-1019. https://doi.org/10.1177/0022034509343413