Odontoblast TRP channels and thermo/mechanical transmission

A. R. Son, Y. M. Yang, J. H. Hong, S. I. Lee, Y. Shibukawa, D. M. Shin

Research output: Contribution to journalArticle

65 Citations (Scopus)

Abstract

Odontoblasts function as mechanosensory receptors because of the expression of mechanosensitive channels in these cells. However, it is unclear if odontoblasts direct the signal transmission evoked by heat/cold or osmotic changes. This study investigated the effects of heat/cold or osmotic changes on calcium signaling and the functional expression of the thermo/mechanosensitive transient receptor potential (TRP) channels in primary cultured mouse odontoblastic cells, with the use of RT-PCR, fluorometric calcium imaging, and electrophysiology. TRPV1, TRPV2, TRPV3, TRPV4, and TRPM3 mRNA was expressed, but TRPM8 and TRPA1 mRNA was not. The receptor-specific stimulation of TRPV1-3 (heat-sensing receptors) and TRPV4/ TRPM3 (mechanic receptors) caused increases in the intracellular calcium concentration. Moreover, the channel activities of TRPV1-4 and TRPM3 were confirmed by a whole-cell patch-clamp technique. These results suggest that primary cultured mouse odontoblasts express heat/mechanosensitive TRP channels and play a role in the underlying mechanisms of thermo/mechanosensitive sensory transmission.

Original languageEnglish
Pages (from-to)1014-1019
Number of pages6
JournalJournal of Dental Research
Volume88
Issue number11
DOIs
Publication statusPublished - 2009 Nov 1

Fingerprint

Transient Receptor Potential Channels
Odontoblasts
Hot Temperature
Calcium
Messenger RNA
Calcium Signaling
Electrophysiology
Patch-Clamp Techniques
Polymerase Chain Reaction

All Science Journal Classification (ASJC) codes

  • Dentistry(all)

Cite this

Son, A. R., Yang, Y. M., Hong, J. H., Lee, S. I., Shibukawa, Y., & Shin, D. M. (2009). Odontoblast TRP channels and thermo/mechanical transmission. Journal of Dental Research, 88(11), 1014-1019. https://doi.org/10.1177/0022034509343413
Son, A. R. ; Yang, Y. M. ; Hong, J. H. ; Lee, S. I. ; Shibukawa, Y. ; Shin, D. M. / Odontoblast TRP channels and thermo/mechanical transmission. In: Journal of Dental Research. 2009 ; Vol. 88, No. 11. pp. 1014-1019.
@article{cc848f54031d4464b2e3c3c9706b7898,
title = "Odontoblast TRP channels and thermo/mechanical transmission",
abstract = "Odontoblasts function as mechanosensory receptors because of the expression of mechanosensitive channels in these cells. However, it is unclear if odontoblasts direct the signal transmission evoked by heat/cold or osmotic changes. This study investigated the effects of heat/cold or osmotic changes on calcium signaling and the functional expression of the thermo/mechanosensitive transient receptor potential (TRP) channels in primary cultured mouse odontoblastic cells, with the use of RT-PCR, fluorometric calcium imaging, and electrophysiology. TRPV1, TRPV2, TRPV3, TRPV4, and TRPM3 mRNA was expressed, but TRPM8 and TRPA1 mRNA was not. The receptor-specific stimulation of TRPV1-3 (heat-sensing receptors) and TRPV4/ TRPM3 (mechanic receptors) caused increases in the intracellular calcium concentration. Moreover, the channel activities of TRPV1-4 and TRPM3 were confirmed by a whole-cell patch-clamp technique. These results suggest that primary cultured mouse odontoblasts express heat/mechanosensitive TRP channels and play a role in the underlying mechanisms of thermo/mechanosensitive sensory transmission.",
author = "Son, {A. R.} and Yang, {Y. M.} and Hong, {J. H.} and Lee, {S. I.} and Y. Shibukawa and Shin, {D. M.}",
year = "2009",
month = "11",
day = "1",
doi = "10.1177/0022034509343413",
language = "English",
volume = "88",
pages = "1014--1019",
journal = "Journal of Dental Research",
issn = "0022-0345",
publisher = "SAGE Publications Inc.",
number = "11",

}

Son, AR, Yang, YM, Hong, JH, Lee, SI, Shibukawa, Y & Shin, DM 2009, 'Odontoblast TRP channels and thermo/mechanical transmission', Journal of Dental Research, vol. 88, no. 11, pp. 1014-1019. https://doi.org/10.1177/0022034509343413

Odontoblast TRP channels and thermo/mechanical transmission. / Son, A. R.; Yang, Y. M.; Hong, J. H.; Lee, S. I.; Shibukawa, Y.; Shin, D. M.

In: Journal of Dental Research, Vol. 88, No. 11, 01.11.2009, p. 1014-1019.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Odontoblast TRP channels and thermo/mechanical transmission

AU - Son, A. R.

AU - Yang, Y. M.

AU - Hong, J. H.

AU - Lee, S. I.

AU - Shibukawa, Y.

AU - Shin, D. M.

PY - 2009/11/1

Y1 - 2009/11/1

N2 - Odontoblasts function as mechanosensory receptors because of the expression of mechanosensitive channels in these cells. However, it is unclear if odontoblasts direct the signal transmission evoked by heat/cold or osmotic changes. This study investigated the effects of heat/cold or osmotic changes on calcium signaling and the functional expression of the thermo/mechanosensitive transient receptor potential (TRP) channels in primary cultured mouse odontoblastic cells, with the use of RT-PCR, fluorometric calcium imaging, and electrophysiology. TRPV1, TRPV2, TRPV3, TRPV4, and TRPM3 mRNA was expressed, but TRPM8 and TRPA1 mRNA was not. The receptor-specific stimulation of TRPV1-3 (heat-sensing receptors) and TRPV4/ TRPM3 (mechanic receptors) caused increases in the intracellular calcium concentration. Moreover, the channel activities of TRPV1-4 and TRPM3 were confirmed by a whole-cell patch-clamp technique. These results suggest that primary cultured mouse odontoblasts express heat/mechanosensitive TRP channels and play a role in the underlying mechanisms of thermo/mechanosensitive sensory transmission.

AB - Odontoblasts function as mechanosensory receptors because of the expression of mechanosensitive channels in these cells. However, it is unclear if odontoblasts direct the signal transmission evoked by heat/cold or osmotic changes. This study investigated the effects of heat/cold or osmotic changes on calcium signaling and the functional expression of the thermo/mechanosensitive transient receptor potential (TRP) channels in primary cultured mouse odontoblastic cells, with the use of RT-PCR, fluorometric calcium imaging, and electrophysiology. TRPV1, TRPV2, TRPV3, TRPV4, and TRPM3 mRNA was expressed, but TRPM8 and TRPA1 mRNA was not. The receptor-specific stimulation of TRPV1-3 (heat-sensing receptors) and TRPV4/ TRPM3 (mechanic receptors) caused increases in the intracellular calcium concentration. Moreover, the channel activities of TRPV1-4 and TRPM3 were confirmed by a whole-cell patch-clamp technique. These results suggest that primary cultured mouse odontoblasts express heat/mechanosensitive TRP channels and play a role in the underlying mechanisms of thermo/mechanosensitive sensory transmission.

UR - http://www.scopus.com/inward/record.url?scp=70350422766&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=70350422766&partnerID=8YFLogxK

U2 - 10.1177/0022034509343413

DO - 10.1177/0022034509343413

M3 - Article

C2 - 19828889

AN - SCOPUS:70350422766

VL - 88

SP - 1014

EP - 1019

JO - Journal of Dental Research

JF - Journal of Dental Research

SN - 0022-0345

IS - 11

ER -