OGFOD1 is required for breast cancer cell proliferation and is associated with poor prognosis in breast cancer

Jae Hwan Kim, Soon Min Lee, Jong Hyuk Lee, Sohyun Chun, Byung Hee Kang, Sojung Kwak, Jae-Seok Roe, Tae Wan Kim, Hyunsoo Kim, Woo Ho Kim, Eun Jung Cho, Hong Duk Youn

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

2-oxogluatrate and Fe(II)-dependent oxygenase domain-containing protein 1 (OGFOD1) was recently revealed to be a proline hydroxylase of RPS23 for translational termination. However, OGFOD1 is nuclear, whereas translational termination occurs in the cytoplasm, raising the possibility of another function of OGFOD1 in the nucleus. In this study, we demonstrate that OGFOD1 is involved in cell cycle regulation. OGFOD1 knockdown in MDA-MB-231 breast cancer cells significantly impeded cell proliferation and resulted in the accumulation of G1 and G2/M cells by decreasing the mRNA levels of G1/S transition- and G2/M-related transcription factors and their target genes. We also confirmed that OGFOD1 is highly expressed in breast cancer tissues by bioinformatic analysis and immunohistochemistry. Thus, we propose that OGFOD1 is required for breast cancer cell proliferation and is associated with poor prognosis in breast cancer.

Original languageEnglish
Pages (from-to)19528-19541
Number of pages14
JournalOncotarget
Volume6
Issue number23
DOIs
Publication statusPublished - 2015 Jan 1

Fingerprint

Cell Proliferation
Breast Neoplasms
Prolyl Hydroxylases
Oxygenases
Computational Biology
Cell Cycle
Cytoplasm
Transcription Factors
Immunohistochemistry
Messenger RNA
Genes

All Science Journal Classification (ASJC) codes

  • Oncology

Cite this

Kim, J. H., Lee, S. M., Lee, J. H., Chun, S., Kang, B. H., Kwak, S., ... Youn, H. D. (2015). OGFOD1 is required for breast cancer cell proliferation and is associated with poor prognosis in breast cancer. Oncotarget, 6(23), 19528-19541. https://doi.org/10.18632/oncotarget.3683
Kim, Jae Hwan ; Lee, Soon Min ; Lee, Jong Hyuk ; Chun, Sohyun ; Kang, Byung Hee ; Kwak, Sojung ; Roe, Jae-Seok ; Kim, Tae Wan ; Kim, Hyunsoo ; Kim, Woo Ho ; Cho, Eun Jung ; Youn, Hong Duk. / OGFOD1 is required for breast cancer cell proliferation and is associated with poor prognosis in breast cancer. In: Oncotarget. 2015 ; Vol. 6, No. 23. pp. 19528-19541.
@article{4f5bc6de13d246959c986be2c1972109,
title = "OGFOD1 is required for breast cancer cell proliferation and is associated with poor prognosis in breast cancer",
abstract = "2-oxogluatrate and Fe(II)-dependent oxygenase domain-containing protein 1 (OGFOD1) was recently revealed to be a proline hydroxylase of RPS23 for translational termination. However, OGFOD1 is nuclear, whereas translational termination occurs in the cytoplasm, raising the possibility of another function of OGFOD1 in the nucleus. In this study, we demonstrate that OGFOD1 is involved in cell cycle regulation. OGFOD1 knockdown in MDA-MB-231 breast cancer cells significantly impeded cell proliferation and resulted in the accumulation of G1 and G2/M cells by decreasing the mRNA levels of G1/S transition- and G2/M-related transcription factors and their target genes. We also confirmed that OGFOD1 is highly expressed in breast cancer tissues by bioinformatic analysis and immunohistochemistry. Thus, we propose that OGFOD1 is required for breast cancer cell proliferation and is associated with poor prognosis in breast cancer.",
author = "Kim, {Jae Hwan} and Lee, {Soon Min} and Lee, {Jong Hyuk} and Sohyun Chun and Kang, {Byung Hee} and Sojung Kwak and Jae-Seok Roe and Kim, {Tae Wan} and Hyunsoo Kim and Kim, {Woo Ho} and Cho, {Eun Jung} and Youn, {Hong Duk}",
year = "2015",
month = "1",
day = "1",
doi = "10.18632/oncotarget.3683",
language = "English",
volume = "6",
pages = "19528--19541",
journal = "Oncotarget",
issn = "1949-2553",
publisher = "Impact Journals",
number = "23",

}

Kim, JH, Lee, SM, Lee, JH, Chun, S, Kang, BH, Kwak, S, Roe, J-S, Kim, TW, Kim, H, Kim, WH, Cho, EJ & Youn, HD 2015, 'OGFOD1 is required for breast cancer cell proliferation and is associated with poor prognosis in breast cancer', Oncotarget, vol. 6, no. 23, pp. 19528-19541. https://doi.org/10.18632/oncotarget.3683

OGFOD1 is required for breast cancer cell proliferation and is associated with poor prognosis in breast cancer. / Kim, Jae Hwan; Lee, Soon Min; Lee, Jong Hyuk; Chun, Sohyun; Kang, Byung Hee; Kwak, Sojung; Roe, Jae-Seok; Kim, Tae Wan; Kim, Hyunsoo; Kim, Woo Ho; Cho, Eun Jung; Youn, Hong Duk.

In: Oncotarget, Vol. 6, No. 23, 01.01.2015, p. 19528-19541.

Research output: Contribution to journalArticle

TY - JOUR

T1 - OGFOD1 is required for breast cancer cell proliferation and is associated with poor prognosis in breast cancer

AU - Kim, Jae Hwan

AU - Lee, Soon Min

AU - Lee, Jong Hyuk

AU - Chun, Sohyun

AU - Kang, Byung Hee

AU - Kwak, Sojung

AU - Roe, Jae-Seok

AU - Kim, Tae Wan

AU - Kim, Hyunsoo

AU - Kim, Woo Ho

AU - Cho, Eun Jung

AU - Youn, Hong Duk

PY - 2015/1/1

Y1 - 2015/1/1

N2 - 2-oxogluatrate and Fe(II)-dependent oxygenase domain-containing protein 1 (OGFOD1) was recently revealed to be a proline hydroxylase of RPS23 for translational termination. However, OGFOD1 is nuclear, whereas translational termination occurs in the cytoplasm, raising the possibility of another function of OGFOD1 in the nucleus. In this study, we demonstrate that OGFOD1 is involved in cell cycle regulation. OGFOD1 knockdown in MDA-MB-231 breast cancer cells significantly impeded cell proliferation and resulted in the accumulation of G1 and G2/M cells by decreasing the mRNA levels of G1/S transition- and G2/M-related transcription factors and their target genes. We also confirmed that OGFOD1 is highly expressed in breast cancer tissues by bioinformatic analysis and immunohistochemistry. Thus, we propose that OGFOD1 is required for breast cancer cell proliferation and is associated with poor prognosis in breast cancer.

AB - 2-oxogluatrate and Fe(II)-dependent oxygenase domain-containing protein 1 (OGFOD1) was recently revealed to be a proline hydroxylase of RPS23 for translational termination. However, OGFOD1 is nuclear, whereas translational termination occurs in the cytoplasm, raising the possibility of another function of OGFOD1 in the nucleus. In this study, we demonstrate that OGFOD1 is involved in cell cycle regulation. OGFOD1 knockdown in MDA-MB-231 breast cancer cells significantly impeded cell proliferation and resulted in the accumulation of G1 and G2/M cells by decreasing the mRNA levels of G1/S transition- and G2/M-related transcription factors and their target genes. We also confirmed that OGFOD1 is highly expressed in breast cancer tissues by bioinformatic analysis and immunohistochemistry. Thus, we propose that OGFOD1 is required for breast cancer cell proliferation and is associated with poor prognosis in breast cancer.

UR - http://www.scopus.com/inward/record.url?scp=84939232113&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84939232113&partnerID=8YFLogxK

U2 - 10.18632/oncotarget.3683

DO - 10.18632/oncotarget.3683

M3 - Article

VL - 6

SP - 19528

EP - 19541

JO - Oncotarget

JF - Oncotarget

SN - 1949-2553

IS - 23

ER -