Oleuropein attenuates hepatic steatosis induced by high-fat diet in mice

Soyoung Park, Youngshim Choi, Soo Jong Um, Seung Kew Yoon, Taesun Park

Research output: Contribution to journalArticle

65 Citations (Scopus)

Abstract

Background & Aims: Oleuropein, a secoiridoid derived from olives and olive oil, has been known to possess antimicrobial, antioxidative, and anticancer activities. The purpose of the present study was to determine whether oleuropein has a protective effect against hepatic steatosis induced by a high fat diet (HFD) and to elucidate its underlying molecular mechanisms in mice. Methods: Male C57BL/6N mice were fed a normal diet (ND), HFD, or an oleuropein-supplemented diet (OSD) for 10 weeks. The plasma and hepatic lipid levels were determined, and the hepatic gene and protein expression levels were analysed via RT-PCR and Western blotting, respectively. Results: The supplementation of HFD with oleuropein reversed the HFD-induced increases in liver weight along with plasma and hepatic lipid levels in mice. The expression of Wnt10b inhibitor genes, such as secreted firizzed-related sequence protein 5 and dickkopf homolog 2, was downregulated, whereas the β-catenin protein expression was upregulated in the liver of OSD-fed mice compared to HFD-fed mice. Fibroblast growth factor receptor 1 (FGFR1), phosphoextracellular-signal- regulated kinase 1/2, cyclin D, and E2F transcription factor 1, along with several key transcription factors and their target genes involved in adipogenesis, were downregulated by oleuropein. OSD-fed mice exhibited decreased expression of the toll-like-receptor-(TLR)-mediated signaling molecules (TLR2, TLR4, and myeloid differentiation primary-response gene 88) and proinflammatory cytokines, in their livers, as compared to HFD mice. Conclusions: These results suggest that the protective effects of oleuropein against HFD-induced hepatic steatosis in mice appear to be associated with the Wnt10b- and FGFR1-mediated signaling cascades involved in hepatic lipogenesis, along with the TLR2- and TLR4-mediated signaling implicated in hepatic steatosis.

Original languageEnglish
Pages (from-to)984-993
Number of pages10
JournalJournal of Hepatology
Volume54
Issue number5
DOIs
Publication statusPublished - 2011 May 1

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High Fat Diet
Liver
Receptor, Fibroblast Growth Factor, Type 1
Diet
Down-Regulation
E2F1 Transcription Factor
oleuropein
Cyclin D
Genes
Lipids
Catenins
Adipogenesis
Lipogenesis
Toll-Like Receptors
Olea
Inbred C57BL Mouse
Proteins
Transcription Factors
Phosphotransferases
Western Blotting

All Science Journal Classification (ASJC) codes

  • Hepatology

Cite this

Park, Soyoung ; Choi, Youngshim ; Um, Soo Jong ; Yoon, Seung Kew ; Park, Taesun. / Oleuropein attenuates hepatic steatosis induced by high-fat diet in mice. In: Journal of Hepatology. 2011 ; Vol. 54, No. 5. pp. 984-993.
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Oleuropein attenuates hepatic steatosis induced by high-fat diet in mice. / Park, Soyoung; Choi, Youngshim; Um, Soo Jong; Yoon, Seung Kew; Park, Taesun.

In: Journal of Hepatology, Vol. 54, No. 5, 01.05.2011, p. 984-993.

Research output: Contribution to journalArticle

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