Olfactory marker protein expression is an indicator of olfactory receptor-associated events in non-olfactory tissues

Na Na Kang, Hyerin Kim, Yoon Gyu Jae, Na Hye Lee, Cheol Ryong Ku, Frank Margolis, Eun Jig Lee, Young Yil Bahk, Min Soo Kim, Jae Hyung Koo

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Olfactory receptor (OR)-associated events are mediated by well-conserved components in the olfactory epithelium, including olfactory G-protein (Golf), adenylate cyclase III (ACIII), and olfactory marker protein (OMP). The expression of ORs has recently been observed in non-olfactory tissues where they are involved in monitoring extracellular chemical cues. The large number of OR genes and their sequence similarities illustrate the need to find an effective and simple way to detect non-olfactory OR-associated events. In addition, expression profiles and physiological functions of ORs in non-olfactory tissues are largely unknown. To overcome limitations associated with using OR as a target protein, this study used OMP with Golf and ACIII as targets to screen for potential OR-mediated sensing systems in non-olfactory tissues. Here, we show using western blotting, real-time PCR, and single as well as double immunoassays that ORs and OR-associated proteins are coexpressed in diverse tissues. The results of immunohistochemical analyses showed OMP (+) cells in mouse heart and in the following cells using the corresponding marker proteins c-kit, keratin 14, calcitonin, and GFAP in mouse tissues: interstitial cells of Cajal of the bladder, medullary thymic epithelial cells of the thymus, parafollicular cells of the thyroid, and Leydig cells of the testis. The expression of ORs in OMP (+) tissues was analyzed using a refined microarray analysis and validated with RT-PCR and real-time PCR. Three ORs (olfr544, olfr558, and olfr1386) were expressed in the OMP (+) cells of the bladder and thyroid as shown using a co-immunostaining method. Together, these results suggest that OMP is involved in the OR-mediated signal transduction cascade with olfactory canonical signaling components between the nervous and endocrine systems. The results further demonstrate that OMP immunohistochemical analysis is a useful tool for identifying expression of ORs, suggesting OMP expression is an indicator of potential OR-mediated chemoreception in non-olfactory systems.

Original languageEnglish
Article numbere0116097
JournalPloS one
Volume10
Issue number1
DOIs
Publication statusPublished - 2015 Jan 30

Fingerprint

Olfactory Marker Protein
Odorant Receptors
olfactory receptors
protein synthesis
Tissue
proteins
Golf
Adenylyl Cyclases
adenylate cyclase
Real-Time Polymerase Chain Reaction
Thyroid Gland
Urinary Bladder
bladder
Keratin-14
cells
Interstitial Cells of Cajal
Proto-Oncogene Proteins c-kit
quantitative polymerase chain reaction
Thymus
Olfactory Mucosa

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

Cite this

Kang, N. N., Kim, H., Jae, Y. G., Lee, N. H., Ku, C. R., Margolis, F., ... Koo, J. H. (2015). Olfactory marker protein expression is an indicator of olfactory receptor-associated events in non-olfactory tissues. PloS one, 10(1), [e0116097]. https://doi.org/10.1371/journal.pone.0116097
Kang, Na Na ; Kim, Hyerin ; Jae, Yoon Gyu ; Lee, Na Hye ; Ku, Cheol Ryong ; Margolis, Frank ; Lee, Eun Jig ; Bahk, Young Yil ; Kim, Min Soo ; Koo, Jae Hyung. / Olfactory marker protein expression is an indicator of olfactory receptor-associated events in non-olfactory tissues. In: PloS one. 2015 ; Vol. 10, No. 1.
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abstract = "Olfactory receptor (OR)-associated events are mediated by well-conserved components in the olfactory epithelium, including olfactory G-protein (Golf), adenylate cyclase III (ACIII), and olfactory marker protein (OMP). The expression of ORs has recently been observed in non-olfactory tissues where they are involved in monitoring extracellular chemical cues. The large number of OR genes and their sequence similarities illustrate the need to find an effective and simple way to detect non-olfactory OR-associated events. In addition, expression profiles and physiological functions of ORs in non-olfactory tissues are largely unknown. To overcome limitations associated with using OR as a target protein, this study used OMP with Golf and ACIII as targets to screen for potential OR-mediated sensing systems in non-olfactory tissues. Here, we show using western blotting, real-time PCR, and single as well as double immunoassays that ORs and OR-associated proteins are coexpressed in diverse tissues. The results of immunohistochemical analyses showed OMP (+) cells in mouse heart and in the following cells using the corresponding marker proteins c-kit, keratin 14, calcitonin, and GFAP in mouse tissues: interstitial cells of Cajal of the bladder, medullary thymic epithelial cells of the thymus, parafollicular cells of the thyroid, and Leydig cells of the testis. The expression of ORs in OMP (+) tissues was analyzed using a refined microarray analysis and validated with RT-PCR and real-time PCR. Three ORs (olfr544, olfr558, and olfr1386) were expressed in the OMP (+) cells of the bladder and thyroid as shown using a co-immunostaining method. Together, these results suggest that OMP is involved in the OR-mediated signal transduction cascade with olfactory canonical signaling components between the nervous and endocrine systems. The results further demonstrate that OMP immunohistochemical analysis is a useful tool for identifying expression of ORs, suggesting OMP expression is an indicator of potential OR-mediated chemoreception in non-olfactory systems.",
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Kang, NN, Kim, H, Jae, YG, Lee, NH, Ku, CR, Margolis, F, Lee, EJ, Bahk, YY, Kim, MS & Koo, JH 2015, 'Olfactory marker protein expression is an indicator of olfactory receptor-associated events in non-olfactory tissues', PloS one, vol. 10, no. 1, e0116097. https://doi.org/10.1371/journal.pone.0116097

Olfactory marker protein expression is an indicator of olfactory receptor-associated events in non-olfactory tissues. / Kang, Na Na; Kim, Hyerin; Jae, Yoon Gyu; Lee, Na Hye; Ku, Cheol Ryong; Margolis, Frank; Lee, Eun Jig; Bahk, Young Yil; Kim, Min Soo; Koo, Jae Hyung.

In: PloS one, Vol. 10, No. 1, e0116097, 30.01.2015.

Research output: Contribution to journalArticle

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T1 - Olfactory marker protein expression is an indicator of olfactory receptor-associated events in non-olfactory tissues

AU - Kang, Na Na

AU - Kim, Hyerin

AU - Jae, Yoon Gyu

AU - Lee, Na Hye

AU - Ku, Cheol Ryong

AU - Margolis, Frank

AU - Lee, Eun Jig

AU - Bahk, Young Yil

AU - Kim, Min Soo

AU - Koo, Jae Hyung

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AB - Olfactory receptor (OR)-associated events are mediated by well-conserved components in the olfactory epithelium, including olfactory G-protein (Golf), adenylate cyclase III (ACIII), and olfactory marker protein (OMP). The expression of ORs has recently been observed in non-olfactory tissues where they are involved in monitoring extracellular chemical cues. The large number of OR genes and their sequence similarities illustrate the need to find an effective and simple way to detect non-olfactory OR-associated events. In addition, expression profiles and physiological functions of ORs in non-olfactory tissues are largely unknown. To overcome limitations associated with using OR as a target protein, this study used OMP with Golf and ACIII as targets to screen for potential OR-mediated sensing systems in non-olfactory tissues. Here, we show using western blotting, real-time PCR, and single as well as double immunoassays that ORs and OR-associated proteins are coexpressed in diverse tissues. The results of immunohistochemical analyses showed OMP (+) cells in mouse heart and in the following cells using the corresponding marker proteins c-kit, keratin 14, calcitonin, and GFAP in mouse tissues: interstitial cells of Cajal of the bladder, medullary thymic epithelial cells of the thymus, parafollicular cells of the thyroid, and Leydig cells of the testis. The expression of ORs in OMP (+) tissues was analyzed using a refined microarray analysis and validated with RT-PCR and real-time PCR. Three ORs (olfr544, olfr558, and olfr1386) were expressed in the OMP (+) cells of the bladder and thyroid as shown using a co-immunostaining method. Together, these results suggest that OMP is involved in the OR-mediated signal transduction cascade with olfactory canonical signaling components between the nervous and endocrine systems. The results further demonstrate that OMP immunohistochemical analysis is a useful tool for identifying expression of ORs, suggesting OMP expression is an indicator of potential OR-mediated chemoreception in non-olfactory systems.

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