Olfactory receptor Olfr544 responding to azelaic acid regulates glucagon secretion in α-cells of mouse pancreatic islets

Nana Kang, Young Yil Bahk, Nahye Lee, Yoongyu Jae, Yoon Hee Cho, Cheol Ryong Ku, Youngjoo Byun, Eunjig Lee, Min Soo Kim, Jaehyung Koo

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Olfactory receptors (ORs) are extensively expressed in olfactory as well as non-olfactory tissues. Although many OR transcripts are expressed in non-olfactory tissues, only a few studies demonstrate the functional role of ORs. Here, we verified that mouse pancreatic α-cells express potential OR-mediated downstream effectors. Moreover, high levels of mRNA for the olfactory receptors Olfr543, Olfr544, Olfr545, and Olfr1349 were expressed in α-cells as assessed using RNA-sequencing, microarray, and quantitative real-time RT-PCR analyses. Treatment with dicarboxylic acids (azelaic acid and sebacic acid) increased intracellular Ca 2+ mobilization in pancreatic α-cells. The azelaic acid-induced Ca 2+ response as well as glucagon secretion was concentration- and time-dependent manner. Olfr544 was expressed in α-cells, and the EC 50 value of azelaic acid to Olfr544 was 19.97 μM, whereas Olfr545 did not respond to azelaic acid. Our findings demonstrate that Olfr544 responds to azelaic acid to regulate glucagon secretion through Ca 2+ mobilization in α-cells of the mouse pancreatic islets, suggesting that Olfr544 may be an important therapeutic target for metabolic diseases.

Original languageEnglish
Pages (from-to)616-621
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume460
Issue number3
DOIs
Publication statusPublished - 2015 May 8

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Odorant Receptors
Glucagon
Islets of Langerhans
Cells
Tissue
RNA Sequence Analysis
Dicarboxylic Acids
Metabolic Diseases
Microarrays
Real-Time Polymerase Chain Reaction
azelaic acid
RNA
Messenger RNA

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Kang, Nana ; Bahk, Young Yil ; Lee, Nahye ; Jae, Yoongyu ; Cho, Yoon Hee ; Ku, Cheol Ryong ; Byun, Youngjoo ; Lee, Eunjig ; Kim, Min Soo ; Koo, Jaehyung. / Olfactory receptor Olfr544 responding to azelaic acid regulates glucagon secretion in α-cells of mouse pancreatic islets. In: Biochemical and Biophysical Research Communications. 2015 ; Vol. 460, No. 3. pp. 616-621.
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Olfactory receptor Olfr544 responding to azelaic acid regulates glucagon secretion in α-cells of mouse pancreatic islets. / Kang, Nana; Bahk, Young Yil; Lee, Nahye; Jae, Yoongyu; Cho, Yoon Hee; Ku, Cheol Ryong; Byun, Youngjoo; Lee, Eunjig; Kim, Min Soo; Koo, Jaehyung.

In: Biochemical and Biophysical Research Communications, Vol. 460, No. 3, 08.05.2015, p. 616-621.

Research output: Contribution to journalArticle

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