On-chip continuous monitoring of motile microorganisms on an ePetri platform

Seung Ah Lee, Guoan Zheng, Nandini Mukherjee, Changhuei Yang

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Self-imaging Petri dish platforms with microscopy resolution, which we term 'ePetri', can significantly streamline cell cultures and/or other longitudinal biological studies. In this paper, we demonstrate high-resolution imaging and long-term culture of motile microorganisms in a specialized ePetri platform by taking advantage of the inherent motion. By applying a super-resolution algorithm to a set of low-resolution images of the microorganisms as they move across the sensing area of a complementary metal oxide semiconductor (CMOS) image sensor chip, we can render an improved-resolution image of the microorganisms. We perform a longitudinal study of Euglena gracilis cultured in an ePetri platform, and image-based analysis on the motion and morphology of the cells. As a miniaturized and automated culture monitoring platform, this ePetri technology can greatly improve studies and experiments with motile microorganisms.

Original languageEnglish
Pages (from-to)2385-2390
Number of pages6
JournalLab on a chip
Volume12
Issue number13
DOIs
Publication statusPublished - 2012 Jul 7

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All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Biochemistry
  • Chemistry(all)
  • Biomedical Engineering

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