On-chip continuous monitoring of motile microorganisms on an ePetri platform

Seung Ah Lee, Guoan Zheng, Nandini Mukherjee, Changhuei Yang

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Self-imaging Petri dish platforms with microscopy resolution, which we term 'ePetri', can significantly streamline cell cultures and/or other longitudinal biological studies. In this paper, we demonstrate high-resolution imaging and long-term culture of motile microorganisms in a specialized ePetri platform by taking advantage of the inherent motion. By applying a super-resolution algorithm to a set of low-resolution images of the microorganisms as they move across the sensing area of a complementary metal oxide semiconductor (CMOS) image sensor chip, we can render an improved-resolution image of the microorganisms. We perform a longitudinal study of Euglena gracilis cultured in an ePetri platform, and image-based analysis on the motion and morphology of the cells. As a miniaturized and automated culture monitoring platform, this ePetri technology can greatly improve studies and experiments with motile microorganisms.

Original languageEnglish
Pages (from-to)2385-2390
Number of pages6
JournalLab on a chip
Volume12
Issue number13
DOIs
Publication statusPublished - 2012 Jul 7

Fingerprint

Microorganisms
Longitudinal Studies
Euglena gracilis
Semiconductors
Monitoring
Image resolution
Oxides
Microscopy
Cell Culture Techniques
Metals
Technology
Imaging techniques
Optical resolving power
Cell culture
Image sensors
Microscopic examination
Experiments

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Biochemistry
  • Chemistry(all)
  • Biomedical Engineering

Cite this

Lee, Seung Ah ; Zheng, Guoan ; Mukherjee, Nandini ; Yang, Changhuei. / On-chip continuous monitoring of motile microorganisms on an ePetri platform. In: Lab on a chip. 2012 ; Vol. 12, No. 13. pp. 2385-2390.
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On-chip continuous monitoring of motile microorganisms on an ePetri platform. / Lee, Seung Ah; Zheng, Guoan; Mukherjee, Nandini; Yang, Changhuei.

In: Lab on a chip, Vol. 12, No. 13, 07.07.2012, p. 2385-2390.

Research output: Contribution to journalArticle

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