On-line high speed lipid extraction for nanoflow liquid chromatography-tandem mass spectrometry

Ju Yong Lee, Joon Seon Yang, Se Mi Park, Seul Kee Byeon, Myeong Hee Moon

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

An on-line lipid extraction method is introduced by utilizing a short capillary extraction column using HILIC and C4 particles prior to nanoflow liquid chromatography-tandem mass spectrometry (nLC–MS/MS). The on-line extraction using a urine sample spiked with PL standards showed similar or slightly higher recovery values (86%–96%) of phospholipids (PLs) compared to those obtained by the conventional off-line extraction based on the Folch method with or without using the air-exposed drying process. In this study, we demonstrated that PL oxidation can occur during the air-exposed drying process of lipid extracts in standard liquid–liquid extraction procedures, which was confirmed by the oxidized PL (OxPL) molecules that were generated from an off-line extraction using a few PL standards. Quantitative comparison of these OxPL species between on- and off-line extraction followed by nLC–MS/MS with multiple reaction monitoring (MRM) analysis showed a significant decrease (2–10 fold) in unwanted OxPL species when on-line extraction was employed. While the number of identified PLs from a urine sample was somewhat lower than those by off-line extraction, the number of OxPLs was significantly reduced (from 70 to 22) with on-line extraction. The new method offers high speed (∼ 5 min) automated extraction of PLs with nLC–MS/MS analysis and presents the possibility of handling a biological sample with a very limited amount of lipids.

Original languageEnglish
Pages (from-to)12-20
Number of pages9
JournalJournal of Chromatography A
Volume1464
DOIs
Publication statusPublished - 2016 Sep 16

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Liquid chromatography
Tandem Mass Spectrometry
Liquid Chromatography
Mass spectrometry
Phospholipids
Lipids
Air
Urine
Drying
Recovery
Oxidation

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

Cite this

Lee, Ju Yong ; Yang, Joon Seon ; Park, Se Mi ; Byeon, Seul Kee ; Moon, Myeong Hee. / On-line high speed lipid extraction for nanoflow liquid chromatography-tandem mass spectrometry. In: Journal of Chromatography A. 2016 ; Vol. 1464. pp. 12-20.
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On-line high speed lipid extraction for nanoflow liquid chromatography-tandem mass spectrometry. / Lee, Ju Yong; Yang, Joon Seon; Park, Se Mi; Byeon, Seul Kee; Moon, Myeong Hee.

In: Journal of Chromatography A, Vol. 1464, 16.09.2016, p. 12-20.

Research output: Contribution to journalArticle

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