Oncogenic role of protein tyrosine kinase 7 in esophageal squamous cell carcinoma

Won Sik Shin, Junhye Kwon, Hae Won Lee, Moon Chul Kang, Hye Won Na, Seung-Taek Lee, Jong Ho Park

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Esophageal squamous cell carcinoma (ESCC) is a common subtype of esophageal cancer that is particularly prevalent in East Asian countries. Our previous expression profile analysis showed that the gene encoding protein tyrosine kinase 7 (PTK7) is upregulated in ESCC tissues. Here, we aimed to validate PTK7 as a prognostic factor and a candidate target for molecular treatment of ESCC. Both RT-PCR and Western blot analysis of tissues from ESCC patients revealed that PTK7 was significantly upregulated in tumor tissue samples of ESCC. Immunohistochemical staining of PTK7 showed that increased expression of PTK7 was inversely correlated with overall survival (P = 0.021). In vitro knockdown of PTK7 inhibited proliferation, survival, wound healing, and invasion of ESCC cells. In addition, PTK7 knockdown decreased phosphorylation of Akt, Erk, and focal adhesion kinase (FAK), important determinants of cell proliferation, survival, and migration. Therefore, our findings suggest that PTK7 has potential as a prognostic marker for ESCC and might also be a candidate for targeted therapy in the treatment of ESCC.

Original languageEnglish
Pages (from-to)1120-1126
Number of pages7
JournalCancer Science
Volume104
Issue number8
DOIs
Publication statusPublished - 2013 Aug 1

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Protein-Tyrosine Kinases
Focal Adhesion Protein-Tyrosine Kinases
Survival
Esophageal Squamous Cell Carcinoma
Esophageal Neoplasms
Wound Healing
Cell Movement
Cell Survival
Therapeutics
Western Blotting
Phosphorylation
Cell Proliferation
Staining and Labeling
Polymerase Chain Reaction
Neoplasms
Proteins

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

Cite this

Shin, W. S., Kwon, J., Lee, H. W., Kang, M. C., Na, H. W., Lee, S-T., & Park, J. H. (2013). Oncogenic role of protein tyrosine kinase 7 in esophageal squamous cell carcinoma. Cancer Science, 104(8), 1120-1126. https://doi.org/10.1111/cas.12194
Shin, Won Sik ; Kwon, Junhye ; Lee, Hae Won ; Kang, Moon Chul ; Na, Hye Won ; Lee, Seung-Taek ; Park, Jong Ho. / Oncogenic role of protein tyrosine kinase 7 in esophageal squamous cell carcinoma. In: Cancer Science. 2013 ; Vol. 104, No. 8. pp. 1120-1126.
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Shin, WS, Kwon, J, Lee, HW, Kang, MC, Na, HW, Lee, S-T & Park, JH 2013, 'Oncogenic role of protein tyrosine kinase 7 in esophageal squamous cell carcinoma', Cancer Science, vol. 104, no. 8, pp. 1120-1126. https://doi.org/10.1111/cas.12194

Oncogenic role of protein tyrosine kinase 7 in esophageal squamous cell carcinoma. / Shin, Won Sik; Kwon, Junhye; Lee, Hae Won; Kang, Moon Chul; Na, Hye Won; Lee, Seung-Taek; Park, Jong Ho.

In: Cancer Science, Vol. 104, No. 8, 01.08.2013, p. 1120-1126.

Research output: Contribution to journalArticle

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AU - Shin, Won Sik

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