One step immobilization of peptides and proteins by using modified parylene with formyl groups

Hyuk Ko, Eun Hang Lee, Ga Yeon Lee, Joil Kim, Byung Jin Jeon, Moo Hwan Kim, Jae Chul Pyun

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

One-step immobilization method for peptides and proteins is developed by using modified parylene film with formyl groups which is suitable for microplate-based immunoassay and SPR biosensor application. The immobilization of peptides and proteins is achieved through the covalent bonding of the formyl group with the primary amine groups of peptides and proteins, which no additional activation step is required. In this work, the immobilization efficiency of parylene-H is estimated in comparison with parylene-A and physical adsorption, using biotinylated-cyclic citrullinated peptide (biotinylated-CCP), human chorionic gonadotropin (hCG) and horseradish peroxidase (HRP) as model proteins. The applicability of parylene-H film to SPR biosensor is demonstrated by estimating the detection range and sensitivity of SPR biosensor at various thicknesses. The immobilization efficiency of parylene-H film for SPR biosensor was compared with physical adsorption by using HRP as a model protein.

Original languageEnglish
Pages (from-to)56-60
Number of pages5
JournalBiosensors and Bioelectronics
Volume30
Issue number1
DOIs
Publication statusPublished - 2011 Dec 15

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Biophysics
  • Biomedical Engineering
  • Electrochemistry

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